Immunofluorescent localization of desmin and vimentin in developing cardiac muscle of Syrian hamster
Tóm tắt
The distributions of desmin and vimentin were examined in frozen sections of cardiac muscle from embryonic, newborn, and adult Syrian hamster by using immunofluorescent methods. Frozen sections of newborn and adult skeletal muscle were used for comparison. Cardiac myocytes from day 9 in utero embryos already show a clear association of desmin with the sarcomeric myofibrils. In newborn hearts, desmin is localized in the myofibrillar Z‐line areas as well as in the peripheral cytoplasm of the cell. Three days after birth, desmin is associated with the intercalated discs. Thus, in adult cardiac muscle, desmin is present in both Z‐bands and intercalated discs. Skeletal muscle of newborn and adult hamster also contains desmin associated with the Z‐lines of myofibrils. Vimentin is associated with the myofibrils of day 9 in utero cardiac muscle cells. The protein remains associated with the myofibrillar Z‐lines in the newborns and adults. No detectable staining for vimentin was observed in newborn or adult hamster skeletal muscle. The existence of vimentin as well as desmin in differentiated cardiac muscle may be a consequence of the somewhat more epithelial‐like nature of cardiac cells as compared to skeletal muscle syncitia.
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Tài liệu tham khảo
Carlsson E., 1982, Differentiation of the myofibrils and the intermediate filament system during postnatal development of the rat heart, Eur. J. Cell Biol., 27, 62
Challice C. E., 1973, Ultra‐structure of the mammalian heart
Lemanski L. F., 1972, 30th Annu. Proc. Electron Microsc
Nag A. C., 1986, Distributions of vimentin and desmin filaments in embryonic cardiac muscle cells in culture, Cytobios, 45, 195
Osinska H. E., 1986, Distribution of desmin in developing hamster skeletal muscle cells, J. Cell Biol., 103, 416a
Osinska H. E., 1986, Localization of desmin and vimentin in developing cardiac muscle of Syrian hamster, J. Cell Biol., 103, 416a
Rumyantsev P. P., 1963, A morphological and autoradiographical study of the peculiarities of differentiation rate, DNA synthesis and nuclear division in the embryonal and postantal histogenesis of cardiac muscle of white cats, Folia Histochem. Cytochem., 1, 463
Samuel J. L., 1985, Myofibrillar examination and desmin in rat heart myocytes, Basic Res. Cardiol., 80, 119
Sandborn B., 1967, Microtubules et filaments cytoplasmiques dans hes muscles de mammiferes, J. Microsc. (Paris), 6, 169
Schliwa M., 1986, The Cytoskeleton. An Introductory Survey
Towbin H., 1979, Electrophoretic transfer of proteins from polyacrylamide gels to introcellulose sheets: Procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350, 10.1073/pnas.76.9.4350
Zak R., 1974, Development and proliferative capacity of cardiac muscle cells, Circ. Res., Suppl. II, 34, II‐17
Zernig G., 1985, Morphological integrity of single adult cardiac myocytes isolated by collagenase treatment: Immuno‐localization of tubulin, microtubule‐associated proteins 1 and 2, plectin, vimentin and vinculin, Eur. J. Cell Biol., 38, 113