Vox Sanguinis
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Simplified Procedure for the Separation of Human T and Non‐T Cells1
Vox Sanguinis - Tập 33 Số 1 - Trang 5-8 - 1977
Abstract. A simple and rapid procedure for the separation of human T and non‐T
lymphocytes is described, based upon an existing technique and using
Ficoll‐Metrizoate. It offers high cell yields, maintenance of a good vitality
and an efficacious separation of the lymphocyte populations mentioned.
Not every PRP‐gel is born equal Evaluation of growth factor availability for tissues through four PRP‐gel preparations: Fibrinet®, RegenPRP‐Kit®, Plateltex® and one manual procedure
Vox Sanguinis - Tập 97 Số 2 - Trang 110-118 - 2009
Background The rationale for using topical platelet gel therapy is to provide
the healing tissues with concentrated platelet‐derived factors. Several systems
are available to prepare platelet‐rich plasma (PRP) and from these, the platelet
gel. These systems produce two‐ to six‐fold platelet and growth factor‐enriched
concentrations. The bioavailability of growth factors in tissue healing depends
...
Platelet content and growth factor release in platelet‐rich plasma: a comparison of four different systems
Vox Sanguinis - Tập 91 Số 2 - Trang 135-139 - 2006
Background Different systems for preparation of platelet‐rich plasma are
commercially available, but data for comparison of these systems have not been
published so far.Materials and Methods We investigated the performance of
Vivostat PRF Preparation Kit®, PCCS Platelet Concentrate Collection System®,
Harvest® SmartPReP 2 APC 60 Process, and Fibrinet® Autologous Fibrin & Platelet
System.The prep...
Transfusion‐associated dyspnoea – shadow or substance?
Vox Sanguinis - Tập 109 Số 2 - Trang 197-200 - 2015
New Zealand Blood Service Haemovigilance uses International Society of Blood
Transfusion/International Haemovigilance Network definitions to categorize
transfusion reactions (TR). Transfusion‐associated dyspnoea (TAD) is a category
for TR with respiratory features (TRRF) that do not fit definitive entities.
TRRF, including TAD, are clinically significant. TR classified as TAD were
reviewed. We fou...
The detection of blood group phenotypes using paper diagnostics
Vox Sanguinis - Tập 108 Số 2 - Trang 186-196 - 2015
Background and ObjectivesPaper biodiagnostics for blood typing are novel, cheap,
fast and easy to use. Agglutinated red blood cells cannot travel through the
porous structure of paper, indicating a positive antibody–antigen interaction
has occurred. Conversely, non‐agglutinated blood can disperse and wick through
the paper structure with the ease to indicate a negative result. This principle
has b...
The Influence of Cryopreservation on Activity and Surface Markers of Lymphokine‐Activated Killer Cells
Vox Sanguinis - Tập 61 Số 2 - Trang 90-95 - 1991
AbstractFicoll‐separated and monocyte‐depleted mononuclear cells isolated from
normal leukapheresis products were cryopreserved. These cells were incubated
with or without 1,000 U/ml of recombinant interleukin‐2 (rIL‐2) for 4 days, and
their lymphokine‐activated killer (LAK) and natural killer (NK) activities were
measured. IL‐2 activation induced a significant increase in the expression of
the CD...
Properties of a new intravenous immunoglobulin (IGIV‐C, 10%) produced by virus inactivation with caprylate and column chromatography
Vox Sanguinis - Tập 84 Số 3 - Trang 193-201 - 2003
Background and Objectives Current manufacture of intravenous immunoglobulin
(Gamimune®N) uses four cold‐ethanol precipitation steps and solvent–detergent
treatment. Our objective was to design a new manufacturing process to maximize
immunoglobulin G (IgG) purity, achieve robust viral safety, preserve all the
biological activities of antibody and avoid unnecessary protein loss.Materials
and Methods...
Studies on the removal of a bovine spongiform encephalopathy‐derived agent by processes used in the manufacture of human immunoglobulin
Vox Sanguinis - Tập 83 Số 2 - Trang 137-145 - 2002
Background and Objectives There is still uncertainty over how the agent of
variant Creutzfeld‐Jakob disease (vCJD) would partition during the manufacture
of plasma derivatives. In this study, a BSE‐derived agent was used as a vCJD
model to determine the extent to which infectivity could be removed by selected
steps used in the manufacture of intravenous immunoglobulin (IVIG).Materials and
Methods ...
Inactivation of West Nile virus, vaccinia virus and viral surrogates for relevant and emergent viral pathogens in plasma‐derived products
Vox Sanguinis - Tập 87 Số 1 - Trang 10-18 - 2004
Background and Objectives Human plasma is the source of a wide variety of
therapeutic proteins, yet it is also a potential source of viral contamination.
Recent outbreaks of emergent viral pathogens, such as West Nile virus, and the
use of live vaccinia virus as a vaccine have prompted a reassessment of the
viral safety of plasma‐derived products. The purpose of this study was to
evaluate the eff...
Intravenous immunoglobulin G: trends in production methods, quality control and quality assurance
Vox Sanguinis - Tập 98 Số 1 - Trang 12-28 - 2010
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