Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf VirusVirologica Sinica - Tập 33 - Trang 173-180 - 2018
Minghao Zhang, Rui Chen, Xueping Zhou, Jianxiang Wu
Wheat dwarf disease caused by wheat dwarf virus (WDV) is currently present in wheat growing regions in China and causes serious losses in wheat yield. To develop reliable and effective serological detection methods for WDV, the coat protein (CP) gene of WDV was cloned and expressed in Escherichia coli. The purified recombinant CP protein was immunized to BALB/c mice, and four hybridoma cell lines ...... hiện toàn bộ
Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and β-d-N4-hydroxycytidineVirologica Sinica - Tập 36 - Trang 997-1005 - 2021
Yuanchao Xie, Xiaozhen Guo, Tianwen Hu, Daibao Wei, Xiuli Ma, Jiaqiang Wu, Bing Huang, Jingshan Shen
Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is widespread in the world. In recent years, the increased virulence of the virus due to viral variations, has caused great economic losses to the pig industry in many countries. It is always worthy to find effective therapeutic methods for PED. As an important class of antivirals, nucleoside drugs which target viral ...... hiện toàn bộ
Construction and genetic analysis of murine hepatitis virus strain A59 Nsp16 temperature sensitive mutant and the revertant virusVirologica Sinica - Tập 26 - Trang 19-29 - 2011
Guo-hui Chang, Bao-jun Luo, Pin Lu, Lei Lin, Xiao-yan Wu, Jing Li, Yi Hu, Qing-yu Zhu
Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study, we created a MHV-A59 temperature sensitive (ts) mutant Wu”-ts18(cd) using the recombinant vaccinia reve...... hiện toàn bộ
Tick-Borne VirusesVirologica Sinica - Tập 33 - Trang 21-43 - 2018
Junming Shi, Zhihong Hu, Fei Deng, Shu Shen
Ticks are important vectors for the transmission of pathogens including viruses. The viruses carried by ticks also known as tick-borne viruses (TBVs), contain a large group of viruses with diverse genetic properties and are concluded in two orders, nine families, and at least 12 genera. Some members of the TBVs are notorious agents causing severe diseases with high mortality rates in humans and li...... hiện toàn bộ
Correction to: Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer CellsVirologica Sinica - Tập 36 - Trang 830-831 - 2021
Jia Dan, Lin Nie, Xudong Jia, Cuiying Xu, Jing Cai, Yuan Lin, Jun Hu, Wenbo Zhu, Yinyin Li, Dong Chen, Ying Liu, Cheng Hu, Guangmei Yan, Jiankai Liang, Qinfen Zhang
Bovine Herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cellsVirologica Sinica - Tập 26 - Trang 403-408 - 2011
Chang Liu, Xiao-hong Kong, Wen-tao Qiao, Yun-qi Geng
The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BH...... hiện toàn bộ
Molecular characterization of China human rabies vaccine strainsVirologica Sinica - Tập 28 - Trang 116-123 - 2013
Xiaoyan Tao, Na Han, Zhenyang Guo, Qing Tang, Simon Rayner, Guodong Liang
To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to thi...... hiện toàn bộ