Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf VirusVirologica Sinica - Tập 33 - Trang 173-180 - 2018
Minghao Zhang, Rui Chen, Xueping Zhou, Jianxiang Wu
Wheat dwarf disease caused by wheat dwarf virus (WDV) is currently present in wheat growing regions in China and causes serious losses in wheat yield. To develop reliable and effective serological detection methods for WDV, the coat protein (CP) gene of WDV was cloned and expressed in Escherichia coli. The purified recombinant CP protein was immunized to BALB/c mice, and four hybridoma cell lines ...... hiện toàn bộ
Construction and genetic analysis of murine hepatitis virus strain A59 Nsp16 temperature sensitive mutant and the revertant virusVirologica Sinica - Tập 26 - Trang 19-29 - 2011
Guo-hui Chang, Bao-jun Luo, Pin Lu, Lei Lin, Xiao-yan Wu, Jing Li, Yi Hu, Qing-yu Zhu
Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study, we created a MHV-A59 temperature sensitive (ts) mutant Wu”-ts18(cd) using the recombinant vaccinia reve...... hiện toàn bộ
Bovine Herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cellsVirologica Sinica - Tập 26 - Trang 403-408 - 2011
Chang Liu, Xiao-hong Kong, Wen-tao Qiao, Yun-qi Geng
The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BH...... hiện toàn bộ
Effect of Loss-of-function of the Herpes Simplex Virus-1 microRNA H6-5p on Virus ReplicationVirologica Sinica - Tập 34 - Trang 386-396 - 2019
Rongquan Huang, Xusha Zhou, Shuqi Ren, Xianjie Liu, Zhiyuan Han, Grace Guoying Zhou
To date, 29 distinct microRNAs (miRNAs) have been reported to be expressed during herpes simplex virus infections. Sequence analysis of mature herpes simplex virus-1 (HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other: miR-H6-5p/H1-3p, miR-H6-3p/H1-5p, H2-5p/H14-3p, miR-H2-3p/H14-5p, and miR-H7/H27. However, the roles of individual miRNAs and consequences of this compl...... hiện toàn bộ
Exploration of a Sequential Gp140-Gp145 Immunization Regimen with Heterologous Envs to Induce a Protective Cross-Reactive HIV Neutralizing Antibody Response In Non-human PrimatesVirologica Sinica - Tập 36 - Trang 784-795 - 2021
Xiangqing Ding, Kangli Cao, Jing Wang, Yanmin Wan, Qinyun Chen, Yanqin Ren, Yongtang Zheng, Mingzhao Zhu, Renrong Tian, Wenjun Wang, Chen Zhao, Xiaoyan Zhang, Jianqing Xu
Raising a heterologous tier 2 neutralizing antibody (nAb) response remains a daunting task for HIV vaccine development. In this study, we explored the utility of diverse HIV-1 envelope (Env) immunogens in a sequential immunization scheme as a solution to this task. This exploration stemmed from the rationale that gp145, a membrane-bound truncation form of HIV Env, may facilitate the focusing of in...... hiện toàn bộ
Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase ProteinVirologica Sinica - Tập 36 - Trang 521-536 - 2020
Xiaoqiang Hao, Yilong Wang, Mengying Zhu, Dongming Zhou, Rongxian Liu, Bin Wang, Yao-Wei Huang, Zhengyan Zhao
Although a live attenuated vaccine is available for controlling mumps virus (MuV), mumps still outbreaks frequently worldwide. The attenuated MuV vaccine strain S79 is widely used in mumps vaccination in China, but still with many shortcomings, among which the most prominent are the side effects and decreased immunity. Therefore, there is a need to further improve the safety and efficacy of the cu...... hiện toàn bộ
In Vitro Infectious Risk Assessment of Heliothis virescens ascovirus 3j (HvAV-3j) toward Non-target Vertebrate CellsVirologica Sinica - Tập 34 - Trang 423-433 - 2019
Huan Yu, Yi-Yi Ou-Yang, Ni Li, Madoka Nakai, Guo-Hua Huang
As specific pathogens of noctuid pests, including Spodoptera exigua, S. litura, Helicoverpa armigera, and Mythimna separata, ascoviruses are suitable for the development of bioinsecticides. In this study, the infectivity of Heliothis virescens ascovirus 3j (HvAV-3j) on insect and mammalian cells was evaluated. HvAV-3j infection induced drastic morphological changes in Sf9, HzAM1, SeFB, and HaFB ce...... hiện toàn bộ