Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf Virus
Tóm tắt
Wheat dwarf disease caused by wheat dwarf virus (WDV) is currently present in wheat growing regions in China and causes serious losses in wheat yield. To develop reliable and effective serological detection methods for WDV, the coat protein (CP) gene of WDV was cloned and expressed in Escherichia coli. The purified recombinant CP protein was immunized to BALB/c mice, and four hybridoma cell lines (i.e. 18G10, 9G4, 23F4 and 22A10) secreting anti-WDV monoclonal antibodies (MAbs) were obtained through the hybridoma technique. Using the prepared MAbs, an antigen-coated-plate enzyme-linked immunosorbent assay (ACP-ELISA) and a dot-ELISA were established for detecting WDV in wheat samples. The most sensitive ACP-ELISA based on MAb 23F4 or 22A10 was able to detect WDV in 1:163,840 (w/v, g/mL) diluted WDV-infected wheat plant crude extracts. The dot-ELISA based on MAb 23F4 was the most sensitive and able to detect the virus in 1:5,120 (w/v, g/mL) diluted wheat plant crude extracts. A total of 128 wheat samples were collected from wheat growing regions in the Shaanxi and Qinghai provinces, China, and were screened for the presence of WDV using two developed serological assays. Results from the survey showed that approximately 62% of the samples were infected with WDV. PCR followed by DNA sequencing and sequence alignment validated the results from the two serological assays. Therefore, we consider that these two serological detection methods can be significantly useful for the control of WDV in China.
Tài liệu tham khảo
Achon MA, Serrano L, Ratti C, Rubies-Autonell C (2006) First detection of wheat dwarf virus in barley in Spain associated with an outbreak of barley yellow dwarf. Plant Dis 90:970
Bendahmane M (1995) Identification and characterization of wheat dwarf virus from france using a rapid method for geminivirus DNA preparation. Phytopathology 85:1449–1455
Chen Z, Zhang MH, Zhou XP, Wu JX (2017) Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus. J Integr Agr 16:115–124
Ekzayez AM, Kumari SG, Ismail I (2010) First report of wheat dwarf virus and its vector (Psammotettix provincialis) affecting wheat and barley crops in Syria. Plant Dis 95:76
Gadiou S, Ripl J, Janourova B, Jarosova J, Kundu JK (2012) Real-time PCR assay for the discrimination and quantification of wheat and barley strains of wheat dwarf virus. Virus Genes 44:349–355
Gawel NJ, Jarret RL (1991) A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol Biol Rep 9:262–266
Jin W, Zhang J, Liu Y, Wang X (2015) Development and application of nucleic acid spot hybridization (NASH) assay for rapid detection of Wheat dwarf virus. Plant Protect 41:100–103 (in Chinese)
Kapooria RG, Ndunguru J (2004) Occurrence of viruses in irrigated wheat in Zambia. EPPO Bull 34:413–419
Koklu G, Ramsell JN, Kvarnheden A (2007) The complete genome sequence for a Turkish isolate of wheat dwarf virus (WDV) from barley confirms the presence of two distinct WDV strains. Virus Genes 34:359–366
Lemmetty A, Huusela-Veistola E (2005) First report of wheat dwarf virus in winter wheat in Finland. Plant Dis 89:912–912
Li N, Chen Z, Liu Y, Liu Y, Zhou XP, Wu JX (2015) Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain. Virol J 12:136
Lindblad M, Sigvald R (2004) Temporal spread of wheat dwarf virus and mature plant resistance in winter wheat. Crop Protect 23:229–234
Liu H, Song XJ, Ni YQ, Lu LN, Zhou XP, Wu JX (2014) Highly sensitive and specific monoclonal antibody-based serological methods for rice ragged stunt virus detection in rice plants and rice brown planthopper vectors. J Integr Agr 13:1943–1951
Liu Z, Chen Z, Hong J, Wang XF, Zhou CY, Zhou XP, Wu JX (2016) Monoclonal antibody-based serological methods for detecting Citrus tristeza virus in citrus groves. Virol Sin 31:324–330
Liu Z, SunZhu YJ, Zhou XP, Hong J, Wu JX (2017) Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves. J Integr Agr 16:884–891
Nygren J, Shad N, Kvarnheden A, Westerbergh A (2015) Variation in susceptibility to wheat dwarf virus among wild and domesticated wheat. PLoS ONE 10:e0121580
Ramsell JNE, Lemmetty A, Jonasson J, Andersson A, Sigvald R, Kvarnheden A (2008) Sequence analyses of Wheat dwarf virus isolates from different hosts reveal low genetic diversity within the wheat strain. Plant Pathol 57:834–841
Schalk HJ, Matzeit V, Schiller B, Schell J, Gronenborn B (1989) Wheat dwarf virus, a geminivirus of graminaceous plants needs splicing for replication. EMBO J 8:359–364
Shang HL, Xie Y, Zhou XP, Qian YJ, Wu JX (2011) Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus. Virol J 8:228
Vacke J (1961) Wheat dwarf virus disease. Biol Plantarum 3:228–233
Wang Y, Mao Q, Liu W, Mar T, Wei T, Liu Y, Wang X (2014) Localization and distribution of Wheat dwarf virus in its vector leafhopper, Psammotettix alienus. Phytopathol 104:897–904
Wang L, Liu Y, Wang XF (2016) Detection of Wheat dwarf virus by TaqMan LNA probe real-time PCR. Acta Phytopathologica Sin 46:313–319 (in Chinese)
Wu J, Ni Y, Liu H, Rao L, Zhou Y, Zhou X (2013) Development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors. Virol J 10:114
Wu J, Ni Y, Liu H, Ding M, Zhou X (2014) Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors. J Virol Methods 195:134–140
Wu B, Shang X, Schubert J, Habekuß A, Elena SF, Wang X (2015) Global-scale computational analysis of genomic sequences reveals the recombination pattern and coevolution dynamics of cereal-infecting geminiviruses. Sci Rep 5:8153
Xie J, Wang X, Liu Y, Peng Y, Zhou G (2007) First report of the occurrence of wheat dwarf virus in wheat in China. Plant Dis 91:111
Zhang X, Zhou G, Wang X (2010) Detection of wheat dwarf virus (WDV) in wheat and vector leafhopper (Psammotettix alienus Dahlb.) by real-time PCR. J Virol Methods 169:416–419
Zhou X, Xie Y, Peng Y, Zhang Z (2003) Malvastrum yellow vein virus, a newBegomovirus species associated with satellite DNA molecule. Chin Sci Bull 48:2206–2210