The Nucleus
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Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f.
The Nucleus - Tập 53 - Trang 89-94 - 2010
Aloe vera L. Burm. f. is used as a popular folk medicine with many cultivars growing naturally or in cultivation in different parts of the world. The present report describes the methods of in vitro propagation and conservation as well as detailed chromosomal analysis of two morphologically indistinguishable cultivars of Aloe vera obtained from NBPGR at the CAZRI (Central Arid Zone Research Instit......
An insight into plant–Tomato leaf curl New Delhi virus interaction
The Nucleus - Tập 60 - Trang 335-348 - 2017
Plants being sessile are constantly exposed to several stresses, which involve different types of abiotic and biotic stress factors. Biotic stress in plants is caused by various living organisms called plant pathogens including bacteria, viruses, fungi and parasites. Among these pathogens, plant viruses cause severe damage to world agricultural productivity. The reason behind such widespread dest......
Ploidy analysis of Helianthus species by flow cytometry and its use in hybridity confirmation
The Nucleus - Tập 59 - Trang 123-130 - 2016
Sunflower (Helianthus annuus L.) is one of the most important oilseed crops cultivated worldwide. It is one of the few crops that has been improved for several characteristics through interspecific hybridization. In the present investigation, the ploidy of 43 Helianthus species belonging to diploid, tetraploid and hexaploid status along with 2 accessions of cultivated sunflower was determined. As ......
Transgenic direct terminal repeats of Dissociation (Ds) produce chromosomal breakage in maize
The Nucleus - - Trang 1-9 - 2023
Barbara McClintock recognized transposable elements originally by the movement of a site of chromosomal breakage, a genetic element called Dissociation (Ds) that was induced to break or transpose by another element she called Activator. The chromosome breaking version, when analyzed on the molecular level was one transposon inside another. It is now known that transposition involving transposon te......
Regulation of autophagy by miRNAs in human diseases
The Nucleus - Tập 64 - Trang 317-329 - 2021
Autophagy is a homeostatic process designed to eliminate dysfunctional and aging organelles and misfolded proteins through a well-concerted pathway, starting with forming a double-membrane vesicle and culminating in the lysosomal degradation of the cargo enclosed inside the mature vesicle. As a vital sentry of cellular health, autophagy is regulated in every human disease condition and is an essen......
Karyomorphological delineation and linear differentiation of microsatellite patterns, and meiosis in giant Asian river frog (Limnonectes blyhii) from Thailand
The Nucleus - Tập 64 - Trang 249-254 - 2020
The present investigation provides information on linear chromosomal differentiation and microsatellite patterns [d(CA)15, d(CGG)10, d(TA)15] in the genome of giant Asian river frog (Limnonectes blyhii). Five male and female samples each collected from southern Thailand, were used for the study. The metaphase chromosome preparations were prepared from the bone marrow using the standard protocol. R......
Karyomorphology of Aspidistra grandiflora H. J. Tillich from Indonesia
The Nucleus - Tập 55 - Trang 139-141 - 2012
The karyotype of Aspidistra grandiflora from Indonesia is described here for the first time. A. grandiflora has a chromosome number of 2n = 38 and is karyotypically formulated as 2n = 17 m + 11sm + 10st. The average chromosome length is 5.07 μm, and the karyotype asymmetry indexes A1 and A2 are respectively 0.44 and 0.60. The cytomorphology of A. grandiflora is remarkably different from those of t......
Modified protocol for obtaining isolated and high-resolution pachytene chromosomes
The Nucleus - Tập 54 - Trang 3-7 - 2011
The expansion of molecular cytogenetics has called for the evaluation of pachytene chromosomes as targets for in situ hybridization (ISH) and micro-dissection. These techniques represent powerful tools for chromosome identification, karyotype comparison, physical mapping and phylogenetic analyses. Because cytogenetic preparations showing well-individualized and cytoplasm-free chromosomes are pre-r......
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