C-heterochromatin and its base composition in holokinetic chromosomes of two species of Heteroptera (Insecta: Hemiptera)
Tóm tắt
Constitutive heterochromatin shows a very diverse distribution pattern and base composition in different species. Serinetha augur (Rhopalidae) and Riptortus pedestris (Alydidae) share the same diploid chromosome complement i.e. 2n = 13 = 10A + 2 m + XO. The banding techniques have been applied for the first time on these two species to compare the distribution pattern and base composition of C-heterochromatin in these species. Serinetha augur and Riptortus pedestris show marked differences in composition and location of C-heterochromatin in autosomes and sex chromosomes. In Serinetha augur, small but distinct sub-terminal C-bands are present whereas in Riptortus pedestris, C-bands are thick and are present at terminal, sub-terminal and interstitial positions. Microchromosomes are C-negative in Serinetha augur but C-positive and CMA3 bright in Riptortus pedestris. Sex chromosome (X) is heavily C-positive in Serinetha augur but shows localized bands in Riptortus pedestris. In Serinetha augur, some C-bands of autosomes are positive for either DAPI or CMA3 while some are positive for both. X is DAPI/CMA3 bright. However, in Riptortus pedestris, all C-bands in the autosomes as well as sex chromosome are DAPI /CMA3 bright indicating overlapping of AT and GC rich DNA in the heterochromatin.
Tài liệu tham khảo
Banerjee MK. A study of the chromosomes during meiosis in twenty-eight species of Hemiptera (Heteroptera, Homoptera). Proc Zool Soc Calcutta. 1959;11:9–37.
Bressa MJ, Franco MJ, Toscani MA, Papeschi AG. Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera:Coreidae). Eur J Entomol. 2008;105:65–72.
Bressa MJ, Larramendy ML, Papeschi AG. Heterochromatin characterization in five species of Heteroptera. Genetica. 2005;124:307–17.
Bressa MJ, Papeschi AG, Mola L, Larramendy ML. Autosomal univalents as a common meiotic feature in Jadera haematoloma and Jadera sanguinolenta (Heteroptera: Rhopalidae: Serinethinae). Eur J Entomol. 2001;98:151–7.
Dey SK, Wangdi T. Banding patterns of the holocentric chromosomes in some species of Heteroptera. Cytologia. 1990;55:181–6.
Kaur H, Chhabra S, Suman V, Gupta M. Chromosomes and their behaviour in two families of the sub-order Heteroptera. Cytologia. 2006;71:111–8.
Kaur H, Suman V, Kaur R. A first report on C-banding and Fluorescent banding in species of Dieuches (Rhyparochrominae: Lygaeidae:Heteroptera). Entomol Res. 2010;40:1–7.
Manicardi GC, Gautam DC. Cytogenetic investigations on the holocentric chromosomes of Tetraneurella akinire (Sasaki) (Homoptera, Pemphigidae). Caryologia. 1994;47:159–65.
Papeschi AG, Bressa MJ. Evolutionary cytogenetics in Heteroptera. Jou Biol Res. 2006;5:3–21.
Sands VE. Cytological studies of the coreidae and alydidae (Hemiptera: Heteroptera). I. Male meiosis in the Malaysian species. Caryologia. 1982;35:291–305.
Sumner AT. A simple technique for demonstrating centromeric heterochromatin. Exptl Cell Res. 1972;75:304–6.