Tạp chí Phân tích Hóa, Lý và Sinh học

Mười dẫn xuất 1,3,4-oxadiazole được tiến hành đánh giá hoạt tính kháng viêm. Kết quả cho thấy các hợp chất 4c-d, và 5 thể hiện hoạt tính ức chế sản sinh NO sử dụng mô hình tế bào RAW264.7 bị kích thích bởi tác nhân LPS với giá trị IC50 trong khoảng từ 58.04 đến 85.31 µM. Kết quả docking phân tử cho thấy các hợp chất 4c-d, và 5 thể hiện ái lực với các enzyme iNOS và nNOS dựa trên giá trị năng lượng liên kết thấp và các tương tác với các amino acid thiết yếu tại các tâm hoạt động của các enzyme.

     Các tính chất phổ 1H và 13C NMR của các hợp chất 6- và 7-alkoxy-4-formyl-coumarin 4-(tetra-O-acetyl-β-D-glucopyranosyl)thiosemicabazon đã được thảo luận. Các tín hiệu phổ được qui kết dựa vào phổ 2D NMR COSY, HSQC và HMBC. Hằng số ghép cặp J = 9,00-9,25 Hz giữa proton H-1' và H-2' trong hợp phần glucopyranose đã xác nhận cấu hình anomer  của các thiosemicarbazone này.

Antioxidant activity is an importance factor for assessing the applicability of plant extracts in the food,functional foods and cosmetics sectors. Codonopsis pilosula Franch. is a tranditional herb wasextracted and freezing dry for storage, evaluation of their antioxidant activity Ampelopsis cantoniensisK.Koch, and Piper betle L. and comapre with extracts of Ampelopsis cantoniensis K.Koch, and Piperbetle L. The antioxidant activity of these herbs were evaluated by total phenolic, total flavonoid, DPPHand ABTS•+radical scavenging methods. Results showed that the total phenolics and flavonoids ofCodonopsis pilosula Franch are smallest among three herbs. The DPPH radical scavenging activity ofAmpelopsis cantoniensis is better than that of other herbs, and has the lowest EC50 values. Ampelopsiscantoniensis K.Koch, and Piper betle L., also showed a high ABTS•+radical cation scavenging activityand the activity of three herb extracts are in the same order as DPPH• radical scavenging activity.Expecially, Ampelopsis cantoniensis and Piper betle have a higher radical scvenging activity thanascorbic acid. Arcoding to the eastern medicine, Codonopsis pilosula Franch. is a medical forimproving health, supporting digestive system, and circulatory system, however, the extract of thisherb did not show noticeable antioxidant activities, it is recomnened to combind with a high antioxidantactivity extract from other herbs for stablizing and protecting bioactive compounds.Keywords: natural antioxidant, Ampelopsis cantoniensis K.Koch, Piper betle L., and Codonopsispilosula Franch.), phenolic, flavonoid, radical scavenging

   In the laboratory, 3 different small-scale constructed wetland systems, a subsurface-flow wetland (SSF), a surface-flow wetland (SF) and a hydroponic system (HP), were characterized regarding the influence of design, distance from the inflow, sampling depth, season, etc. on the purification of a model acid mine drainage (AMD). In three constructed wetlands, the mean removal rates were 32 and 15% for acidity, 23 and 6% for sulfate, 58 and 38% for Fe, and 32 and 28% for Zn.  In general, the processing efficiency of the highest SSF system, followed by SF and final system is HP system. In the summer more efficiently handle the winter, at a depth of 15 cm better than 25 cm. 

In this study, we have successfully developed the Cu2MoS4/SPE-based electrochemical sensor for determining the Chloramphenicol (CAP) in pharmaceutical samples. The Cu2MoS4/SPE-based electrochemical sensor shows the linear range from 1.0 to 50 μM with electrochemical sensitivity of 0.219 μA/μM. Furthermore, the proposed electrochemical sensor has been tested to determine the CAP content in the Chloramphenicol 250mg drug with relative errors of 3.87%. The obtained results demonstrate the promising applications of Cu2MoS4 in developing advanced electrochemical sensors.

Bacillus anthracis represents a serious threat of biological weapons, and there is an urgent need to develop a rapid, simple and sensitive assay to detect B. anthracis spores in environmental samples. Commercial lateral flow immunoassays for detection of B. anthracis spores are easy to perform but exhibit very low sensitivity. This study aims to develop a sensitive and simple dipstick test, based on agglutination reaction between B. anthracis spores and antibody-coated magnetic particles, to detect spores of this pathogen. To this aim, factors affecting the test performance including antibody, nitrocellulose membrane type, incubation time and pH of reaction between detection conjugate and sample, and concentration of Tween-20 were optimized. Under the optimal condition (monoclonal antibody to Anthrax Spore Specific 23A-14G9, UniSart® CN95 nitrocellulose membrane, 30 minutes of incubation, pH 9.0, and 1% Tween-20), the developed test could detect B. anthracis spores with the limit of detection of 6 × 104spores/mL, and showed no cross-reactivity to B. thuringiensis, B. cereus, B. subtilis, B. clausii, Yersinia bercovier và Yersinia rohdei and E. coli. When applied to detect B. anthracis spores in simulated starch samples, the developed assay has a detection limit of 107spores/g starch. This new immunoassay is particularly suitable for screening of B. anthracis spores in powdery samples because of its simplicity and improved sensitivity.

    A tetrakis terbi (III) complex [Tb(BTFAC)3(phen)] (BTFAC- is benzoyltrifluoroacetateand phen is 1, 10- phenanthroline) was synthesized and studied by the method ofinfrared spectroscopy and single crystal X -ray diffraction. The results showed thatthe complex is mononuclear, in which three BTFAC- and one phen ligandscoordinated to a terbi(III) ion.Keywords: Rare earth, β- diketone, complexes.