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Oestrogen-dependent expression of the SM2 smooth muscle-type myosin isoform in rabbit myometrium
Springer Science and Business Media LLC - Tập 18 - Trang 413-427 - 1997
Ovarectomized rabbits displayed a decreased SM1 to SM2 ratio of smooth muscle-type myosin heavy chain isoforms compared to unoperated, virgin females which was reversed after 17β- oestradiol administration to a value similar to that of control animals. When this steroid was given to sexually immature animals or to adult virgin rabbits, SM2 expression was not induced, as also happened with prolifer......
Force transients and minimum cross-bridge models in muscular contraction
Springer Science and Business Media LLC - Tập 28 - Trang 371-395 - 2008
Two- and three-state cross-bridge models are considered and examined with respect to their ability to predict three distinct phases of the force transients that occur in response to step change in muscle fiber length. Particular attention is paid to satisfying the Le Châtelier–Brown Principle. This analysis shows that the two-state model can account for phases 1 and 2 of a force transient, but is ......
Human single masseter muscle fibers contain unique combinations of myosin and myosin binding protein C isoforms
Springer Science and Business Media LLC - Tập 23 - Trang 317-326 - 2002
Striated craniofacial and limb muscles differ in their embryological origin, regulatory program during myogenesis, and innervation. In an attempt to explore the effects of these differences on the striated muscle phenotype in humans, the expression of myosin and myosin-associated thick filament proteins were studied at the single fiber level both in the human jaw-closing masseter muscle and in two......
Characterization of connectin-like proteins of obliquely striated muscle of a polychaete (Annelida)
Springer Science and Business Media LLC - Tập 15 - Trang 623-632 - 1994
In obliquely striated muscle of polychaete, Neanthes sp., three kinds of connectin (titin)-like high molecular weight proteins, ∼4000 kDa, ∼1200 kDa and ∼700 kDa, were detected by SDS gel electrophoresis and immunoblots using antibodies to vertebrate skeletal muscle connectin and antiserum to the protein in question. The 700 kDa protein was isolated and characterized as a β sheet-rich filament 170......
The signaling mechanisms of long distance intercellular calcium waves (far waves)in cultured human uterine myocytes
Springer Science and Business Media LLC - Tập 23 - Trang 279-284 - 2002
Cultured human myocytes exhibit intercellular calcium waves that travel farther than 100 μm (‘far waves’). This work investigates the mechanism of far wave propagation. Culture lines were initiated from myometrial biopsies of term pregnant women. Calcium green-1 was used as a fluorescence probe for intracellular free calcium. Serial imaging was performed at a frame rate of 0.83 frames/s. Intercell......
Effects of 2,3-butanedione monoxime on the crossbridge kinetics in frog single muscle fibres
Springer Science and Business Media LLC - Tập 13 - Trang 516-522 - 1992
The effects of 2,3-butanedione monoxime (BDM) on contraction characteristics were studied at 5‡C in single intact fibres isolated from the tibialis anterior muscle of the frog. The force-velocity relation was determined using the controlled-velocity method in either whole fibres or short fibre segments in which sarcomere shortening was measured by a laser light diffraction method. It is shown that......
Myosin isozymes in avian skeletal muscles. I. Sequential expression of myosin isozymes in developing chicken pectoralis muscles
Springer Science and Business Media LLC - Tập 4 - Trang 695-716 - 1983
Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide mapping of partial chymotryptic digests showed, however, that its heavy chain is qu......
Prolonged passive stretch of rat soleus muscle provokes an increase in the mRNA levels of the muscle regulatory factors distributed along the entire length of the fibers
Springer Science and Business Media LLC - Tập 20 - Trang 395-402 - 1999
The mRNA levels of the adult and the neonatal sarcoplasmic/endoplasmic reticulum Ca2+-ATPases (SERCA1a and SERCA1b, respectively) and those of the muscle regulatory factors (MRFs: myoD, myf-5, myogenin, MRF4) have been assessed by RT PCR in rat soleus muscles immobilized for 3 days in an extended position (passive stretch). The transcript level of the fast type SERCA1a Ca2+-transport ATPase decrea......
Calcium transients in skeletal muscle fibres under isometric conditions and during and after a quick stretch
Springer Science and Business Media LLC - - 1991
Mechanical and kinetic properties of β-cardiac/slow skeletal muscle myosin
Springer Science and Business Media LLC - Tập 33 - Trang 403-417 - 2012
We aimed to establish reference parameters to identify functional effects of familial hypertrophic cardiomyopathy-related point mutations in the β-cardiac/slow skeletal muscle myosin heavy chain (β-cardiac/MyHC-1). We determined mechanical and kinetic parameters of the β-cardiac/MyHC-1 using human soleus muscle fibers that express the same myosin heavy chain (MyHC-1) as ventricular myocardium (β-c......
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