Characterization of connectin-like proteins of obliquely striated muscle of a polychaete (Annelida)
Tóm tắt
In obliquely striated muscle of polychaete, Neanthes sp., three kinds of connectin (titin)-like high molecular weight proteins, ∼4000 kDa, ∼1200 kDa and ∼700 kDa, were detected by SDS gel electrophoresis and immunoblots using antibodies to vertebrate skeletal muscle connectin and antiserum to the protein in question. The 700 kDa protein was isolated and characterized as a β sheet-rich filament 170 nm long and 4 nm wide. Using polyclonal antibodies to the 700 kDa protein, the binding of the immunogold to the thick filament was only demonstrated in high ionic strength relaxing solution which solubilized some myosin. This observation suggested that the 700 kDa protein was localized below the layers of myosin in the thick filament and this localization is different from that of twitchin of C. elegans bodywall muscle that is on the surface of thick filament. The 4000 kDa protein was identified as a very thin filament linking the thick filament to the dense body. The very thin filaments were visualized in gelsolin-treated actin filament-free fibres. The 1200 kDa protein was located in the periphery of the dense body. A model of the elastic filament in polychaete bodywall muscle is presented.
Tài liệu tham khảo
BENIAN, G. M., KIFF, J. E., NECKELMANN, N., MOERMAN, D. G. & WATERSTON, R. H. (1989) Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans. Nature 342, 45–50.
Benian G. M., L'Hernault S. W., Fox L. A., Tucker C. Y. & Sale W. S. (1991) Structure of twitchin: an unusually large sarcomeric protein of C. elegans. J. Cell Biol. 115, 29a.
BENIAN, G. M., L'HERNAULT, S. W. & MORRIS, M. E. (1993) Additional sequence complexity in the muscle gene, unc-22, and its encoded protein, twitchin, of Caenorhabditis elegans. Genetics 134, 1097–104.
FUNATSU, T., HIGUCHI, H. & ISHIWATA, S. (1990) Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin. J. Cell Biol. 110, 53–62.
HU, D. H., MATSUNO, A., TERAKADO, K., MATSUURA, T., KIMMURA, S. & MARUYAMA, K. (1990) Projectin is an invertebrate connectin (titin): isolation from crayfish claw muscle and localization in crayfish claw muscle and insect flight muscle. J. Muscle Res. Cell Motil. 11, 497–511.
ITOH, Y., SUZUKI, T., KIMURA, S., OHASHI, K., HIGUCHI, H., SAWADA, H., SHIMIZU, T., SHIBATA, M. & MARUYAMA, K. (1988) Extensible and less-extensible domains of connectin filaments in stretched vertebrate skeletal muscle sarcomers as detected by immunofluorescence and immunoelectron microscopy using monoclonal antibodies. J. Biochem. 104, 504–8.
LAEMMLI, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–5.
LAKEY, A., FERGUSON, C., LABEIT, S., REEDY, M., LARKINS, A., BUTCHER, G., LAONARD, K. & BULLARD, B. (1990) Identification and localization of high molecular weight proteins in insect flight and leg muscle. EMBO J. 9, 3459–67.
LAKEY, A., LABEIT, S., GAUTEL, M., FERGUSON, C., BARLOW, D., LEONARD, K. & BULLARD, B. (1993) Kettin, a large modular protein in the Z-disc of insect muscle. EMBO J. 12, 2863–71.
LANZAVECCHIA, G. (1977) Morphological modulations in helical muscles (Aschelminthes and Annelida). Int. Rev. Cytol. 51, 133–86.
MANABE, T., KAWAMURA, Y., HIGUTI, H., KIMURA, S. & MARUYAMA, K. (1993) Connectin, giant elastic protein, in giant sarcomeres of crayfish claw muscle. J. Muscle Res. Cell Motil. 14, 654–65.
MARUYAMA, K. (1994) Connectin, an elastic protein of striated muscle. Biophys. Chem. 50, 73–85.
MATSUMURA, K., SHIMIZU, T., MANNEN, T. & MARUYAMA, K. (1989) The immunological homology between two filamentous cross-linker phosphoproteins, connectin and cross-bridge region of neurofilament-H, is not affected by the phosphorylation state. J. Biochem. 105, 226–30.
MATSUNO, A. & KAWAMURA, Y. (1991) Cell types differing in thick myofilament diameter in obliquely striated muscle of a polychaete annelid, Neanthes. Tissue Cell 23, 481–7.
MATSUNO, A., TAKANO-OHMURO, H., ITHO, Y., MATSUURA, T., SHIBATA, M., NAKAE, H., KAMINUMA, T. & MARUYAMA, K. (1989) Anti-connectin monoclonal antibodies that react with the unc-22 gene product bind dense bodies of Caenorhabditis (nematode) bodywall muscle cells. Tissue Cell 21, 495–505.
MOERMAN, D. G., BENIAN, G. M., BARSTEAD, R. J., SCHRIEFER, L. A. & WATERSTON, R. H. (1988) Identification and intracellular localization of the unc-22 gene product of Caenorhabditis elegans. Genes Development 2, 93–105.
MATSUMURA, T., KIMURA, S., OHTSUKA, S. & MARUYAMA, K. (1991) Isolation and characterization of 1,200 kDa peptide of α-connectin. J. Biochem. 110, 474–8.
NAVE, R., FÜRST, D. & WEBER, K. (1989) Visualization of the polarity of isolated titin molecules: a single globular head on a long thin rod as the M band anchoring domain? J. Cell Biol. 109, 2177–87.
NAVE, R. & WEBER, K. (1990) A myofibrillar protein of insect muscle related to vertebrate titin connects Z-band and A-band: purification and molecular characterization of invertebrate mini-titin. J. Cell Sci. 95, 535–44.
NAVE, R., FÜRST, D., VINKEMEIER, U. & WEBER, K. (1991) Purification and physical properties of nematode mini-titins and their relation to twitchin. J. Cell Sci. 98, 491–6.
PROVENCHER, S. W. & GLÖCKNER, J. (1981) Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20, 33–7.
ROSENBLUTH, J. (1972) Obliquely striated muscle. The Structure and Function of Muscle, vol. 1 (edited by BOURNE, G. H.) pp. 389–420. New York: Academic Press.
SAIDE, J. D. (1981) Identification of a connecting filament in insect fibrillar flight muscle. J. Mol. Biol. 153, 661–79.
TRINICK, J. (1992) Understanding the functions of titin and nebulin. FEBS Letts 307, 44–8.
VIBERT, P., EDELSTEIN, S. M., CASTELLANI, L. & ELLIOTT, B. W. (1993) Mini-titin in striated and smooth molluscan muscles: structure, location and immunological crossreactivity. J. Muscle Res. Cell Motil. 14, 598–607.
VIGOREAUX, J. O. (1994) The muscle Z band: lessons in stress management. J. Muscle Res. Cell Motil. 15, 237–55.