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Microglial inclusions and neurofilament light chain release follow neuronal α-synuclein lesions in long-term brain slice cultures
Springer Science and Business Media LLC - Tập 16 - Trang 1-17 - 2021
Melanie Barth, Mehtap Bacioglu, Niklas Schwarz, Renata Novotny, Janine Brandes, Marc Welzer, Sonia Mazzitelli, Lisa M. Häsler, Manuel Schweighauser, Thomas V. Wuttke, Deborah Kronenberg-Versteeg, Karina Fog, Malene Ambjørn, Ania Alik, Ronald Melki, Philipp J. Kahle, Derya R. Shimshek, Henner Koch, Mathias Jucker, Gaye Tanriöver
Proteopathic brain lesions are a hallmark of many age-related neurodegenerative diseases including synucleinopathies and develop at least a decade before the onset of clinical symptoms. Thus, understanding of the initiation and propagation of such lesions is key for developing therapeutics to delay or halt disease progression. Alpha-synuclein (αS) inclusions were induced in long-term murine and human slice cultures by seeded aggregation. An αS seed-recognizing human antibody was tested for blocking seeding and/or spreading of the αS lesions. Release of neurofilament light chain (NfL) into the culture medium was assessed. To study initial stages of α-synucleinopathies, we induced αS inclusions in murine hippocampal slice cultures by seeded aggregation. Induction of αS inclusions in neurons was apparent as early as 1week post-seeding, followed by the occurrence of microglial inclusions in vicinity of the neuronal lesions at 2–3 weeks. The amount of αS inclusions was dependent on the type of αS seed and on the culture’s genetic background (wildtype vs A53T-αS genotype). Formation of αS inclusions could be monitored by neurofilament light chain protein release into the culture medium, a fluid biomarker of neurodegeneration commonly used in clinical settings. Local microinjection of αS seeds resulted in spreading of αS inclusions to neuronally connected hippocampal subregions, and seeding and spreading could be inhibited by an αS seed-recognizing human antibody. We then applied parameters of the murine cultures to surgical resection-derived adult human long-term neocortical slice cultures from 22 to 61-year-old donors. Similarly, in these human slice cultures, proof-of-principle induction of αS lesions was achieved at 1week post-seeding in combination with viral A53T-αS expressions. The successful translation of these brain cultures from mouse to human with the first reported induction of human αS lesions in a true adult human brain environment underlines the potential of this model to study proteopathic lesions in intact mouse and now even aged human brain environments.
ZNStress: a high-throughput drug screening protocol for identification of compounds modulating neuronal stress in the transgenic mutant sod1G93R zebrafish model of amyotrophic lateral sclerosis
Springer Science and Business Media LLC - - 2016
Alexander McGown, Dame Pamela J. Shaw, Tennore Ramesh
Amyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease with death on average within 2–3 years of symptom onset. Mutations in superoxide dismutase 1 (SOD1) have been identified to cause ALS. Riluzole, the only neuroprotective drug for ALS provides life extension of only 3 months on average. Thishighlights the need for compound screening in disease models to identify new neuroprotective therapies for this disease. Zebrafish is an emerging model system that is well suited for the study of diseasepathophysiology and also for high throughput (HT) drug screening. The mutant sod1 zebrafish model of ALS mimics the hallmark features of ALS. Using a fluorescence based readout of neuronal stress, we developed a high throughput (HT) screen to identify neuroprotective compounds. Here we show that the zebrafish screen is a robust system that can be used to rapidly screen thousands ofcompounds and also demonstrate that riluzole is capable of reducing neuronal stress in this model system. The screen shows optimal quality control, maintaining a high sensitivity and specificity withoutcompromising throughput. Most importantly, we demonstrate that many compounds previously failed in human clinical trials, showed no stress reducing activity in the zebrafish assay. We conclude that HT drug screening using a mutant sod1 zebrafish is a reliable model system which supplemented with secondary assays would be useful in identifying drugs with potential for neuroprotective efficacy in ALS.
Correction: Unravelling cell type-specific responses to Parkinson’s Disease at single cell resolution
Springer Science and Business Media LLC - - 2024
Araks Martirosyan, Rizwan Ansari, Francisco Pestana, Katja Hebestreit, Hayk Gasparyan, Razmik Aleksanyan, Silvia Hnatova, Suresh Poovathingal, Catherine Marneffe, Dietmar R. Thal, Andrew Kottick, Victor J. Hanson-Smith, Sebastian Guelfi, William Plumbly, T. Grant Belgard, Emmanouil Metzakopian, Matthew G. Holt
Cảnh quan biểu hiện và sự đồng điều chỉnh của các gen lão hóa đặc hiệu theo mô và loại tế bào ở người Dịch bởi AI
Springer Science and Business Media LLC - - 2022
Peng Xu, Minghui Wang, Won-min Song, Qian Wang, Guo-Cheng Yuan, Peter H. Sudmant, Habil Zare, Zhidong Tu, Miranda E. Orr, Bin Zhang
Lão hóa tế bào là một phản ứng căng thẳng phức tạp ảnh hưởng đến chức năng tế bào và sức khỏe của cơ thể. Nhiều yếu tố phát triển và môi trường, chẳng hạn như tín hiệu nội tại của tế bào, bức xạ, căng thẳng oxy hóa, gen ung thư và sự tích tụ protein, kích hoạt các gen và con đường có thể dẫn đến lão hóa. Đã có những nỗ lực to lớn để xác định và đặc trưng hóa các gen lão hóa (SnGs) trong các hệ thống căng thẳng và bệnh tật. Tuy nhiên, sự phổ biến của các tế bào lão hóa trong các mô khỏe mạnh của con người và chữ ký biểu hiện SnG toàn cầu ở các loại tế bào khác nhau chưa được hiểu rõ. Nghiên cứu này đã thực hiện một phân tích mạng lưới gen tích hợp dữ liệu RNA-seq của tế bào đơn và môi trường tổng thể trong các mô người không bị bệnh để điều tra chữ ký đồng biểu hiện SnG và tính đặc hiệu của chúng theo loại tế bào. Thông qua phân tích mạng lưới phiên mã toàn diện của 50 mô người trong dự án Biểu hiện Genotype-Tissue Expression (GTEx), chúng tôi đã xác định các mô đun gen giàu SnG, đặc trưng hóa các mẫu đồng biểu hiện SnG và xây dựng các mạng lưới SnG tổng hợp trên các mô chính của cơ thể người. Các phương pháp mạng lưới của chúng tôi đã xác định 51 SnG được bảo tồn cao ở tất cả các mô người, bao gồm cả các điều hòa viên tập trung vào CDKN1A (p21) điều khiển tiến trình chu kỳ tế bào và kiểu hình tiết liên quan đến lão hóa (SASP). Các mô đun giàu SnG cho thấy tính đặc hiệu theo loại tế bào đáng kể, đặc biệt là ở sợi bào, tế bào nội mô và tế bào miễn dịch. Các phân tích thêm về dữ liệu RNA-seq của tế bào đơn và dữ liệu phiên mã không gian đã xác thực độc lập những chữ ký SnG đặc hiệu theo loại tế bào được dự đoán bởi phân tích mạng. Nghiên cứu này đã tiết lộ có hệ thống các tổ chức đồng điều chỉnh và tính đặc hiệu theo loại tế bào của SnGs trong các mô chính của con người, điều này có thể phục vụ như một bản thiết kế cho các nghiên cứu trong tương lai để lập bản đồ các tế bào lão hóa và các tương tác tế bào của chúng trong các mô người.
#lão hóa tế bào #gen lão hóa #phân tích mạng lưới gen #RNA-seq #mô người #tính đặc hiệu theo loại tế bào
A validated antibody panel for the characterization of tau post-translational modifications
Springer Science and Business Media LLC - Tập 12 - Trang 1-19 - 2017
Ebru Ercan, Sameh Eid, Christian Weber, Alexandra Kowalski, Maria Bichmann, Annika Behrendt, Frank Matthes, Sybille Krauss, Peter Reinhardt, Simone Fulle, Dagmar E. Ehrnhoefer
Tau is a microtubule-binding protein, which is subject to various post-translational modifications (PTMs) including phosphorylation, methylation, acetylation, glycosylation, nitration, sumoylation and truncation. Aberrant PTMs such as hyperphosphorylation result in tau aggregation and the formation of neurofibrillary tangles, which are a hallmark of Alzheimer’s disease (AD). In order to study the importance of PTMs on tau function, antibodies raised against specific modification sites are widely used. However, quality control of these antibodies is lacking and their specificity for particular modifications is often unclear. In this study, we first designed an online tool called ‘TauPTM’, which enables the visualization of PTMs and their interactions on human tau. Using TauPTM, we next searched for commercially available antibodies against tau PTMs and characterized their specificity by peptide array, immunoblotting, electrochemiluminescence ELISA and immunofluorescence technologies. We demonstrate that commercially available antibodies can show a significant lack of specificity, and PTM-specific antibodies in particular often recognize non-modified versions of the protein. In addition, detection may be hindered by other PTMs in close vicinity, complicating the interpretation of results. Finally, we compiled a panel of specific antibodies and show that they are useful to detect PTM-modified endogenous tau in hiPSC-derived neurons and mouse brains. This study has created a platform to reliably and robustly detect changes in localization and abundance of post-translationally modified tau in health and disease. A web-based version of TauPTM is fully available at http://www.tauptm.org .
Pathological phosphorylation of tau and TDP-43 by TTBK1 and TTBK2 drives neurodegeneration
Springer Science and Business Media LLC - - 2018
Laura M. Taylor, Pamela J. McMillan, Nicole F. Liachko, Timothy J. Strovas, Bernardino Ghetti, Thomas D. Bird, C. Dirk Keene, Brian C. Kraemer
An exo-cell assay for examining real-time γ-secretase activity and inhibition
Springer Science and Business Media LLC - Tập 4 - Trang 1-9 - 2009
Christopher C Shelton, Yuan Tian, Mark G Frattini, Yue-Ming Li
γ-Secretase is an aspartyl protease that cleaves multiple substrates that are involved in broad biological processes ranging from stem cell development to neurodegeneration. The investigation of γ-secretase has been limited by currently available assays that require genetic or biochemical manipulation in the form of substrate transfection or membrane preparation. Here we report an exo-cell assay that is capable of characterizing γ-secretase activity in any cellular system without limitation. Using a highly active, recombinant substrate this assay can quickly and easily ascertain the status of γ-secretase activity in cell systems and patient samples. We have applied this method to determine the activity of γ-secretase in primary cell samples where transfection and/or membrane isolation are not viable options. Importantly, it allows for the detection of real time γ-secretase activity after inhibitor or drug treatment. The application of this assay to determine the role of γ-secretase in physiological and pathological conditions will greatly facilitate our characterization of this complex protease and help in the development and evaluation of γ-secretase-targeted therapies in Alzheimer's disease or a variety of neoplasms.
The 5th International Conference on Molecular Neurodegeneration: Overlapping Pathologies and Common Mechanisms
Springer Science and Business Media LLC - Tập 14 - Trang 1-73 - 2019
Immunotherapy targeting toll-like receptor 2 alleviates neurodegeneration in models of synucleinopathy by modulating α-synuclein transmission and neuroinflammation
Springer Science and Business Media LLC - Tập 13 - Trang 1-18 - 2018
Changyoun Kim, Brian Spencer, Edward Rockenstein, Hodaka Yamakado, Michael Mante, Anthony Adame, Jerel Adam Fields, Deborah Masliah, Michiyo Iba, He-Jin Lee, Robert A. Rissman, Seung-Jae Lee, Eliezer Masliah
Synucleinopathies of the aging population are an heterogeneous group of neurological disorders that includes Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) and are characterized by the progressive accumulation of α-synuclein in neuronal and glial cells. Toll-like receptor 2 (TLR2), a pattern recognition immune receptor, has been implicated in the pathogenesis of synucleinopathies because TLR2 is elevated in the brains of patients with PD and TLR2 is a mediator of the neurotoxic and pro-inflammatory effects of extracellular α-synuclein aggregates. Therefore, blocking TLR2 might alleviate α-synuclein pathological and functional effects. For this purpose, herein, we targeted TLR2 using a functional inhibitory antibody (anti-TLR2). Two different human α-synuclein overexpressing transgenic mice were used in this study. α-synuclein low expresser mouse (α-syn-tg, under the PDGFβ promoter, D line) was stereotaxically injected with TLR2 overexpressing lentivirus to demonstrate that increment of TLR2 expression triggers neurotoxicity and neuroinflammation. α-synuclein high expresser mouse (α-Syn-tg; under mThy1 promoter, Line 61) was administrated with anti-TLR2 to examine that functional inhibition of TLR2 ameliorates neuropathology and behavioral defect in the synucleinopathy animal model. In vitro α-synuclein transmission live cell monitoring system was used to evaluate the role of TLR2 in α-synuclein cell-to-cell transmission. We demonstrated that administration of anti-TLR2 alleviated α-synuclein accumulation in neuronal and astroglial cells, neuroinflammation, neurodegeneration, and behavioral deficits in an α-synuclein tg mouse model of PD/DLB. Moreover, in vitro studies with neuronal and astroglial cells showed that the neuroprotective effects of anti-TLR2 antibody were mediated by blocking the neuron-to-neuron and neuron-to-astrocyte α-synuclein transmission which otherwise promotes NFκB dependent pro-inflammatory responses. This study proposes TLR2 immunotherapy as a novel therapeutic strategy for synucleinopathies of the aging population.
A C9ORF72 BAC mouse model recapitulates key epigenetic perturbations of ALS/FTD
Springer Science and Business Media LLC - Tập 12 - Trang 1-11 - 2017
Rustam Esanov, Gabriela Toro Cabrera, Nadja S. Andrade, Tania F. Gendron, Robert H. Brown, Michael Benatar, Claes Wahlestedt, Christian Mueller, Zane Zeier
Amyotrophic Lateral Sclerosis (ALS) is a fatal and progressive neurodegenerative disorder with identified genetic causes representing a significant minority of all cases. A GGGGCC hexanucleotide repeat expansion (HRE) mutation within the C9ORF72 gene has recently been identified as the most frequent known cause of ALS. The expansion leads to partial heterochromatinization of the locus, yet mutant RNAs and dipeptide repeat proteins (DPRs) are still produced in sufficient quantities to confer neurotoxicity. The levels of these toxic HRE products positively correlate with cellular toxicity and phenotypic severity across multiple disease models. Moreover, the degree of epigenetic repression inversely correlates with some facets of clinical presentation in C9-ALS patients. Recently, bacterial artificial chromosomes (BAC) have been used to generate transgenic mice that harbor the HRE mutation, complementing other relevant model systems such as patient-derived induced pluripotent stem cells (iPSCs). While epigenetic features of the HRE have been investigated in various model systems and post-mortem tissues, epigenetic dysregulation at the expanded locus in C9-BAC mice remains unexplored. Here, we sought to determine whether clinically relevant epigenetic perturbations caused by the HRE are mirrored in a C9-BAC mouse model. We used complementary DNA methylation assessment and immunoprecipitation methods to demonstrate that epigenetic aberrations caused by the HRE, such as DNA and histone methylation, are recapitulated in the C9-BAC mice. Strikingly, we found that cytosine hypermethylation within the promoter region of the human transgene occurred in a subset of C9-BAC mice similar to what is observed in patient populations. Moreover, we show that partial heterochromatinization of the C9 HRE occurs during the first weeks of the mouse lifespan, indicating age-dependent epigenetic repression. Using iPSC neurons, we found that preventing R-loop formation did not impede heterochromatinization of the HRE. Taken together, these observations provide further insight into mechanism and developmental time-course of epigenetic perturbations conferred by the C9ORF72 HRE. Finally, we suggest that epigenetic repression of the C9ORF72 HRE and nearby gene promoter could impede or delay motor neuron degeneration in C9-BAC mouse models of ALS/FTD.
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