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Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Springer Science and Business Media LLC - Tập 13 - Trang 963-970 - 2011
Yan Zhao, Qin Liu, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang
Displaying foreign antigens on the surface of attenuated or avirulent bacteria is an important strategy to develop live multivalent vector vaccines. In our previous work, several efficient surface display systems have been established based on outer membrane anchoring elements, which could successfully display heterologous proteins in attenuated Vibrio anguillarum. In this work, the glyceraldehyde...... hiện toàn bộ
Complete Mitochondrial DNA Sequence of Tigriopus japonicus (Crustacea: Copepoda)
Springer Science and Business Media LLC - Tập 4 - Trang 406-417 - 2002
Ryuji J. Machida, Masaki U. Miya, Mutsumi Nishida, Shuhei Nishida
The complete nucleotide sequence of the mitochondrial genome was determined for a harpacticoid copepod, Tigriopus japonicus (Crustacea), using an approach that employs a long polymerase chain reaction technique and primer walking. Although the genome (14,628 bp) contained the same set of 37 genes (2 ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in other metazoan animals, no...... hiện toàn bộ
Antifouling Activity of Meroterpenes Isolated from the Ascidian Aplidium aff. densum
Springer Science and Business Media LLC - Tập 23 - Trang 51-61 - 2020
Annabel Levert, Valentin Foulon, Marilyne Fauchon, Nathalie Tapissier-Bontemps, Bernard Banaigs, Claire Hellio
The settlement and growth of fouling organisms on man-made surfaces can be prevented by the application of antifouling paints containing active compounds (biocides, heavy metals), most of which are toxic to non-target organisms. As part of our research program in chemical ecology and blue biotechnology, we are conducting studies to investigate the natural defence mechanisms of marine organisms tha...... hiện toàn bộ
Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
Springer Science and Business Media LLC - Tập 25 - Trang 657-665 - 2022
Insaf Naser, Yusuke Yabu, Yoshiaki Maeda, Tsuyoshi Tanaka
The oleaginous diatom Fistulifera solaris is a promising producer of biofuel owing to the high content of the lipids. A genetic transformation technique by microparticle bombardment for this diatom was already established. However, the transformation efficiency was significantly lower than those of other diatoms. Devoting efforts to advance the genetic modifications of this diatom is crucial to un...... hiện toàn bộ
Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon)
Springer Science and Business Media LLC - Tập 4 - Trang 487-494 - 2013
Premruethai Supungul, Sirawut Klinbunga, Rath Pichyangkura, Sarawut Jitrapakdee, Ikuo Hirono, Takashi Aoki, Anchalee Tassanakajon
An expressed sequence tag (EST) library was constructed from hemocytes of the black tiger shrimp (Penaeus monodon) to identify genes associated with immunity in this economically important species. The number of complementary DNA clones in the constructed library was approximately 4 × 105. Of these, 615 clones having inserts larger than 500 by were unidirectionally sequenced and analyzed by homolo...... hiện toàn bộ
scRNA-seq Analysis of Hemocytes of Penaeid Shrimp Under Virus Infection
Springer Science and Business Media LLC - Tập 25 - Trang 488-502 - 2023
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
The classification of cells in non-model organisms has lagged behind the classification of cells in model organisms that have established cluster of differentiation marker sets. To reduce fish diseases, research is needed to better understand immune-related cells, or hemocytes, in non-model organisms like shrimp and other marine invertebrates. In this study, we used Drop-seq to examine how virus i...... hiện toàn bộ
Isolation and Characterization of Tetranucleotide Microsatellites from Atlantic Haddock (Melanogrammus aeglefinus)
Springer Science and Business Media LLC - Tập 4 - Trang 418-422 - 2002
Patrick T. O'Reilly, Arran A. McPherson, Ellen Kenchington, Chris Taggart, Mathew W. Jones, Paul Bentzen
Five tetranucleotide microsatellite loci developed from Atlantic haddock (Melanogrammus aeglefinus) are presented. Loci were isolated using a modified magnetic bead-hybridization selection procedure that enriched for tetranucleotide microsatellites. Loci were polymorphic (3–99 alleles per locus; mean, 33.2) and exhibited high levels of observed heterozygosity (0.07–0.99; mean, 0.73) in a sample of...... hiện toàn bộ
Tích hợp các phương pháp Biểu hiện gen và Protein học cung cấp một tập hợp gen cốt lõi để hiểu rõ về sự bám dính của ngao Dịch bởi AI
Springer Science and Business Media LLC - Tập 17 - Trang 523-532 - 2015
Yan Miao, Lingling Zhang, Yan Sun, Wenqian Jiao, Yangping Li, Jin Sun, Yangfan Wang, Shi Wang, Zhenmin Bao, Weizhi Liu
Sự bám dính là một quá trình sinh lý thiết yếu trong lịch sử đời sống của nhiều sinh vật biển. Sử dụng sự kết hợp giữa các phương pháp biểu hiện gen và protein học, các protein bám dính ngao (Sbps) cùng với các gen trong mạng lưới điều chỉnh của chúng đã được nghiên cứu lần đầu tiên. Chúng tôi đã xây dựng thư viện biểu hiện gen chân ngao đầu tiên, và xác định được 75 gen chuyên biệt cho chân ngao....... hiện toàn bộ
#ngao #protein bám dính #biểu hiện gen #protein học #sửa đổi sau dịch mã
Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production
Springer Science and Business Media LLC - Tập 18 - Trang 1-14 - 2015
Ge-Yi Fu, Yi Lu, Zhe Chi, Guang-Lei Liu, Shou-Feng Zhao, Hong Jiang, Zhen-Ming Chi
In this study, a pyruvate carboxylase gene (PYC1) from a marine fungus Penicillium rubens I607 was cloned and characterized. ORF of the gene (accession number: KM397349.1) had 3534 bp encoding 1177 amino acids with a molecular weight of 127.531 kDa and a PI of 6.20. The promoter of the gene was located at −1200 bp and contained a TATAA box, several CAAT boxes and a sequence 5′-SYGGRG-3′. The PYC1 ...... hiện toàn bộ
Effects of 5-Aminolevulinic Acid on Gene Expression, Immunity, and ATP Levels in Pacific White Shrimp, Litopenaeus vannamei
Springer Science and Business Media LLC - Tập 20 Số 6 - Trang 829-843 - 2018
Ivane R. Pedrosa‐Gerasmio, Tohru Tanaka, Asuka Sumi, Hidehiro Kondo, Ikuo Hirono
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