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Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei
Springer Science and Business Media LLC - Tập 22 - Trang 423-432 - 2020
Yue Wang, Yang Yu, Shihao Li, Xiaojun Zhang, Jianhai Xiang, Fuhua Li
In order to learn the sex determination and differentiation process in shrimp, we performed sex-specific transcriptome sequencing of the Zoea I larvae in Litopenaeus vannamei (L. vannamei) using a DNA/RNA co-extraction method. The sex-specific genes and the sex-linked SNPs were identified. De novo assembly of all the clean reads generated 41,270 unigenes with an average length of 1026 bp and an N5...... hiện toàn bộ
Acute Salinity Stress Disrupts Gut Microbiota Homeostasis and Reduces Network Connectivity and Cooperation in Razor Clam Sinonovacula constricta
Springer Science and Business Media LLC - Tập 25 - Trang 1147-1157 - 2023
Wenfang Dai, Zijuan Zhang, Yinghui Dong, Lin He, Qinggang Xue, Zhihua Lin
Accumulating evidence demonstrates that it is of great importance to maintain a stable and functional gut microbial community for host’s growth and health. However, gut microenvironment is constantly affected by diverse environmental factors. Salinity can cause stress, including hypersaline or hyposaline stress to aquatic species, thereby affecting their growth conditions. Razor clam (Sinonovacula...... hiện toàn bộ
Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Springer Science and Business Media LLC - Tập 13 - Trang 963-970 - 2011
Yan Zhao, Qin Liu, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang
Displaying foreign antigens on the surface of attenuated or avirulent bacteria is an important strategy to develop live multivalent vector vaccines. In our previous work, several efficient surface display systems have been established based on outer membrane anchoring elements, which could successfully display heterologous proteins in attenuated Vibrio anguillarum. In this work, the glyceraldehyde...... hiện toàn bộ
Complete Mitochondrial DNA Sequence of Tigriopus japonicus (Crustacea: Copepoda)
Springer Science and Business Media LLC - Tập 4 - Trang 406-417 - 2002
Ryuji J. Machida, Masaki U. Miya, Mutsumi Nishida, Shuhei Nishida
The complete nucleotide sequence of the mitochondrial genome was determined for a harpacticoid copepod, Tigriopus japonicus (Crustacea), using an approach that employs a long polymerase chain reaction technique and primer walking. Although the genome (14,628 bp) contained the same set of 37 genes (2 ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in other metazoan animals, no...... hiện toàn bộ
Generation of Albino Phenotype in Ornamental Fish by CRISPR/Cas9-Mediated Genome Editing of slc45a2 Gene
Springer Science and Business Media LLC - Tập 25 - Trang 281-290 - 2023
Changqing Zhang, Ziheng Ren, Zhiyuan Gong
Albinism is the most common color variation described in fish and is a fascinating trait of some ornamental fish species. Albino mutants can be generated by knocking out core genes affecting melanin synthesis like slc45a2 in several fish species. However, genetic mutation remains challenging for species with unknown genome information. In this study, we generated albino mutants in two selected orn...... hiện toàn bộ
Biocalcification by Piezotolerant Bacillus sp. NIOTVJ5 Isolated from Deep Sea Sediment and its Influence on the Strength of Concrete Specimens
Springer Science and Business Media LLC - Tập 21 - Trang 161-170 - 2018
Vijaya Raghavan Rangamaran, Venkat Kumar Shanmugam
Biocalcification or microbially induced carbonate precipitation (MICP) is gaining attention from the research fraternity, primarily ascribed to their eco-friendly applications. Bacterial strains have been isolated from various sources and their ability to precipitate carbonate has been studied extensively. In spite of the fact that the deep-sea environment is a potential source for bioprospecting,...... hiện toàn bộ
Cloning and Sequencing of Alginate Lyase Genes from Deep-Sea Strains of Vibrio and Agarivorans and Characterization of a New Vibrio Enzyme
Springer Science and Business Media LLC - - 2010
Kohsuke Uchimura, Masaru Miyazaki, Yuichi Nogi, Tohru Kobayashi, Koki Horikoshi
Effects of Formate on Fermentative Hydrogen Production by Enterobacter aerogenes
Springer Science and Business Media LLC - Tập 7 - Trang 112-118 - 2005
Tatsuo Kurokawa, Shigeharu Tanisho
This paper describes the effects of formate on fermentative hydrogen production by Enterobacter aerogenes by way of batch culture. When 20 mM formate was added to pH 6.3 and pH 5.8 E. aerogenes glucose cultures (formate culture) at the beginning of cultivation, hydrogen evolution through both glucose consumption and decomposition of the extrinsic formate occurred together, while hydrogen evolution...... hiện toàn bộ
Antifouling Activity of Meroterpenes Isolated from the Ascidian Aplidium aff. densum
Springer Science and Business Media LLC - Tập 23 - Trang 51-61 - 2020
Annabel Levert, Valentin Foulon, Marilyne Fauchon, Nathalie Tapissier-Bontemps, Bernard Banaigs, Claire Hellio
The settlement and growth of fouling organisms on man-made surfaces can be prevented by the application of antifouling paints containing active compounds (biocides, heavy metals), most of which are toxic to non-target organisms. As part of our research program in chemical ecology and blue biotechnology, we are conducting studies to investigate the natural defence mechanisms of marine organisms tha...... hiện toàn bộ
Highly Efficient Genetic Transformation Methods for the Marine Oleaginous Diatom Fistulifera solaris
Springer Science and Business Media LLC - Tập 25 - Trang 657-665 - 2022
Insaf Naser, Yusuke Yabu, Yoshiaki Maeda, Tsuyoshi Tanaka
The oleaginous diatom Fistulifera solaris is a promising producer of biofuel owing to the high content of the lipids. A genetic transformation technique by microparticle bombardment for this diatom was already established. However, the transformation efficiency was significantly lower than those of other diatoms. Devoting efforts to advance the genetic modifications of this diatom is crucial to un...... hiện toàn bộ
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