Isolation and Characterization of Tetranucleotide Microsatellites from Atlantic Haddock (Melanogrammus aeglefinus)

Springer Science and Business Media LLC - Tập 4 - Trang 418-422 - 2002
Patrick T. O'Reilly1, Arran A. McPherson1, Ellen Kenchington2, Chris Taggart1, Mathew W. Jones3, Paul Bentzen3
1Department of Oceanography, Dalhousie University, Halifax, NS, Canada B3H 4J1, , CA
2Fisheries & Oceans Canada, Bedford Institute of Oceanography, Dartmouth NS, Canada B2Y 4A2, , CA
3Marine Gene Probe Laboratory, Dalhousie University, Halifax, NS, Canada B3H 4J1, , CA

Tóm tắt

Five tetranucleotide microsatellite loci developed from Atlantic haddock (Melanogrammus aeglefinus) are presented. Loci were isolated using a modified magnetic bead-hybridization selection procedure that enriched for tetranucleotide microsatellites. Loci were polymorphic (3–99 alleles per locus; mean, 33.2) and exhibited high levels of observed heterozygosity (0.07–0.99; mean, 0.73) in a sample of 70 haddock collected from the Scotian Shelf in the Northwest Atlantic Ocean. Primer sets for 5 tetranucleotide microsatellites, originally developed from cod (Gmo34), were also tested in Atlantic haddock; one pair yielded readily detectable product and was variable in the population assayed (29 alleles; heterozygosity, 0.96). These loci are suitable for kinship analyses in aquaculture-related applications, and are potentially useful for resolving population structure in the wild.