A trypsin-like protease with apparent dual function in early Lepeophtheirus salmonis (Krøyer) developmentSpringer Science and Business Media LLC - Tập 10 - Trang 1-11 - 2009
Rasmus Skern-Mauritzen, Petter Frost, Sussie Dalvin, Bjørn Olav Kvamme, Ingunn Sommerset, Frank Nilsen
Trypsin-like serine proteases are involved in a large number of processes including digestive degradation, regulation of developmental processes, yolk degradation and yolk degradome activation. Trypsin like peptidases considered to be involved in digestion have been characterized in Lepeophtheirus salmonis. During these studies a trypsin-like peptidase which differed in a number of traits were ide...... hiện toàn bộ
Characterization, phylogeny, alternative splicing and expression of Sox30 geneSpringer Science and Business Media LLC - Tập 11 - Trang 1-11 - 2010
Fei Han, Zhijian Wang, Fengrui Wu, Zhihao Liu, Baofeng Huang, Deshou Wang
Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its exact function remains unclear. Sox30 cDNA was cloned from the Nile tilapia by RT-PCR and RA...... hiện toàn bộ
Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphateSpringer Science and Business Media LLC - Tập 9 - Trang 1-10 - 2008
Andreas Knödler, Gerlinde Konrad, Peter Mayinger
Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cer...... hiện toàn bộ
Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus sungorusSpringer Science and Business Media LLC - Tập 8 - Trang 1-15 - 2007
Tobias Fromme, Kathrin Reichwald, Matthias Platzer, Xing-Sheng Li, Martin Klingenspor
Ucp3 is an integral protein of the inner mitochondrial membrane with a role in lipid metabolism preventing deleterious effects of fatty acids in states of high lipid oxidation. Ucp3 is expressed in brown adipose tissue and skeletal muscle and controlled by a transcription factor complex including PPARalpha, MyoD and the histone acetyltransferase p300. Several studies have demonstrated interaction ...... hiện toàn bộ
The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasisSpringer Science and Business Media LLC - Tập 12 - Trang 1-15 - 2011
David CF Sealey, Aleksandar D Kostic, Catherine LeBel, Fiona Pryde, Lea Harrington
The first telomerase-associated protein (Est1) was isolated in yeast due to its essential role in telomere maintenance. The human counterparts EST1A, EST1B, and EST1C perform diverse functions in nonsense-mediated mRNA decay (NMD), telomere length homeostasis, and telomere transcription. Although Est1 and EST1A/B interact with the catalytic subunit of yeast and human telomerase (Est2 and TERT, res...... hiện toàn bộ
Temperature-induced variation in gene expression burst size in metazoan cellsSpringer Science and Business Media LLC - Tập 16 - Trang 1-11 - 2015
Ophélie Arnaud, Sam Meyer, Elodie Vallin, Guillaume Beslon, Olivier Gandrillon
Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanisms of the underlying microscopic molecular system. By using different clones of chicken erythroid progenitor cells harboring different integration sites of a CMV-driven mCherry protein, we in...... hiện toàn bộ
Gene expression profiles in skeletal muscle after gene electrotransferSpringer Science and Business Media LLC - Tập 8 - Trang 1-12 - 2007
Pernille Hojman, John R Zibert, Hanne Gissel, Jens Eriksen, Julie Gehl
Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres. We have therefore investigated transcriptional changes through gene expression profile analyses, morphological changes by h...... hiện toàn bộ
Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysisSpringer Science and Business Media LLC - Tập 8 - Trang 1-14 - 2007
Štefan Čikoš, Alexandra Bukovská, Juraj Koppel
Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method chosen for data analysis can strongly influence results of the quantification. To compare the performance of six techniques which are currently used for analysing fluorescent data in real-time PCR relative quantification, we quantified four cytok...... hiện toàn bộ
Glycogen Synthase Kinase-3 regulates IGFBP-1 gene transcription through the Thymine-rich Insulin Response ElementSpringer Science and Business Media LLC - Tập 5 - Trang 1-13 - 2004
David Finlay, Satish Patel, Lorna M Dickson, Natalia Shpiro, Rodolfo Marquez, Chris J Rhodes, Calum Sutherland
Hepatic expression of several gene products involved in glucose metabolism, including phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6Pase) and insulin-like growth factor binding protein-1 (IGFBP-1), is rapidly and completely inhibited by insulin. This inhibition is mediated through the regulation of a DNA element present in each of these gene promoters, that we call the T hymi...... hiện toàn bộ