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A trypsin-like protease with apparent dual function in early Lepeophtheirus salmonis (Krøyer) development
Springer Science and Business Media LLC - Tập 10 - Trang 1-11 - 2009
Trypsin-like serine proteases are involved in a large number of processes including digestive degradation, regulation of developmental processes, yolk degradation and yolk degradome activation. Trypsin like peptidases considered to be involved in digestion have been characterized in Lepeophtheirus salmonis. During these studies a trypsin-like peptidase which differed in a number of traits were ide......
Characterization, phylogeny, alternative splicing and expression of Sox30 gene
Springer Science and Business Media LLC - Tập 11 - Trang 1-11 - 2010
Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its exact function remains unclear. Sox30 cDNA was cloned from the Nile tilapia by RT-PCR and RA......
Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
Springer Science and Business Media LLC - Tập 9 - Trang 1-10 - 2008
Phosphoinositides play a central role in regulating processes at intracellular membranes. In yeast, a large number of phospholipid biosynthetic enzymes use a common mechanism for transcriptional regulation. Yet, how the expression of genes encoding lipid kinases and phosphatases is regulated remains unknown. Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cer......
An optimized rapid bisulfite conversion method with high recovery of cell-free DNA
Springer Science and Business Media LLC - Tập 18 Số 1 - 2017
TRPC1 transcript variants, inefficient nonsense-mediated decay and low up-frameshift-1 in vascular smooth muscle cells
Springer Science and Business Media LLC - Tập 12 - Trang 1-10 - 2011
Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. Extensive TRPC1 transcript alternative splicing was observed, with exons 2, 3 and 5-9 frequently omitted, leading to variants c......
Hfq stimulates the activity of the CCA-adding enzyme
Springer Science and Business Media LLC - Tập 8 - Trang 1-7 - 2007
The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP). As a member of the nucleotidyltransferase superfamily, this enzyme shares a high sequence similarity with an other representative of this family, the tRNA nucleotidyl......
Prediction and preliminary validation of oncogene regulation by miRNAs
Springer Science and Business Media LLC - Tập 8 - Trang 1-14 - 2007
MicroRNAs (miRNAs) are one of the most abundant groups of regulatory genes in multicellular organisms, playing important roles in many fundamental cellular processes. More than four hundred miRNAs have been identified in humans and the deregulation of miRNA expression has been also shown in many cancers. Despite the postulated involvement of miRNAs in tumourigenesis, there are only a few examples ......
Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus sungorus
Springer Science and Business Media LLC - Tập 8 - Trang 1-15 - 2007
Ucp3 is an integral protein of the inner mitochondrial membrane with a role in lipid metabolism preventing deleterious effects of fatty acids in states of high lipid oxidation. Ucp3 is expressed in brown adipose tissue and skeletal muscle and controlled by a transcription factor complex including PPARalpha, MyoD and the histone acetyltransferase p300. Several studies have demonstrated interaction ......
Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator
Springer Science and Business Media LLC - Tập 5 - Trang 1-13 - 2004
Foamy virus Bel1/Tas trans-activators act as key regulators of gene expression and directly bind to Bel1 response elements (BRE) in both the internal and the 5'LTR promoters leading to strong transcriptional trans-activation. Cellular coactivators interacting with Bel1/Tas are unknown to date. Transient expression assays, co-immunoprecipitation experiments, pull-down assays, and Western blot analy......
PlasmaDNA: a free, cross-platform plasmid manipulation program for molecular biology laboratories
Springer Science and Business Media LLC - Tập 8 - Trang 1-5 - 2007
Most molecular biology experiments, and the techniques associated with this field of study, involve a great deal of engineering in the form of molecular cloning. Like all forms of engineering, perfect information about the starting material is crucial for successful completion of design and strategies. We have generated a program that allows complete in silico simulation of the cloning experiment.......
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