Precise identification of chicken chromosomes in the lampbrush form using chromosome painting probesSpringer Science and Business Media LLC - Tập 11 - Trang 749-757 - 2003
Svetlana Derjusheva, Anna Kurganova, Alla Krasikova, Alsu Saifitdinova, Felix A. Habermann, Elena Gaginskaya
Chromosome painting probes specific for macrochromosomes 1, 2, 3, 4, 5, and Z were applied to both mitotic and lampbrush chromosomes of the chicken (Gallus gallus domesticus). Five autosomal macrobivalents and sex chromosome Z in the lampbrush phase were identified and their correspondence to the target chromosomes in the metaphase of mitosis was shown. Nascent transcripts on lateral loops of the ...... hiện toàn bộ
Highly distinct chromosomal structures in cowpea (Vigna unguiculata), as revealed by molecular cytogenetic analysisSpringer Science and Business Media LLC - Tập 24 - Trang 197-216 - 2016
Aiko Iwata-Otsubo, Jer-Young Lin, Navdeep Gill, Scott A. Jackson
Cowpea (Vigna unguiculata (L.) Walp) is an important legume, particularly in developing countries. However, little is known about its genome or chromosome structure. We used molecular cytogenetics to characterize the structure of pachytene chromosomes to advance our knowledge of chromosome and genome organization of cowpea. Our data showed that cowpea has highly distinct chromosomal structures tha...... hiện toàn bộ
Nature of B chromosomes in the harvest mouse Reithrodontomys megalotis by fluorescence in situ hybridization (FISH)Springer Science and Business Media LLC - Tập 5 - Trang 475-479 - 1997
John A. Peppers, Lara E. Wiggins, Robert J. Baker
Using fluorescence in situ hybridization, we examined the characteristics of two types of B chromosomes in harvest mice of the genus Reithrodontomys. B chromosomes were interrogated with rDNA, telomeric repeat, LINE element and centromeric heterochromatin probes. The two types of B chromosomes share the following features: (a) telomeres present on the ends of both arms; (b) hybridization to LINE p...... hiện toàn bộ
Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?Springer Science and Business Media LLC - Tập 27 - Trang 129-140 - 2018
Helena Fulka, Alena Langerova
Nucleoli are the site of ribosomal RNA production and subunit assembly. In contrast to active nucleoli in somatic cells, where three basic sub-compartments can be observed, mammalian oocytes and early embryos contain atypical nucleoli termed “nucleolus-like bodies” or “nucleolus precursor bodies”, respectively. Unlike their somatic counterparts, these structures are composed of dense homogenous fi...... hiện toàn bộ
Localization of seed protein genes on flow-sorted field bean chromosomesSpringer Science and Business Media LLC - Tập 1 - Trang 107-115 - 1993
Jiří Macas, Jaroslav Doležel, Sergio Lucretti, Uta Pich, Armin Meister, Jörg Fuchs, Ingo Schubert
Chromosomes from reconstructed field bean (Vicia faba L.) karyotypes were flow-sorted and the DNA was used for the physical localization of seed storage and nonstorage (USP) protein genes using PCR with sequence specific primers. The data were confirmed and refined by using DNA of microisolated chromosomes of other karyotypes as the target for PCR. The specificity of the PCR products was proved by...... hiện toàn bộ
Sister Chromatid Differentiation With Biotin-dUTPSpringer Science and Business Media LLC - Tập 7 - Trang 185-189 - 1999
Elisabeth Bruckmann, Andrzej Wojcik, Günter Obe
The method of choice to differentiate sister chromatids is to incorporate BrdU in replicating DNA. The disadvantage of BrdU is that its spontaneous or induced radicalization may itself lead to sister chromatid exchanges. Biotin-labelled dUTP is a widely used thymidine analogon for labelling isolated DNA. Its chemical structure suggests that, in contrast to BrdU, it does not give rise to radical fo...... hiện toàn bộ
Nuclear architecture and the structural basis of mitotic memorySpringer Science and Business Media LLC - Tập 31 - Trang 1-23 - 2023
Mamilla Soujanya, Ashish Bihani, Nikhil Hajirnis, Rashmi U. Pathak, Rakesh K. Mishra
The nucleus is a complex organelle that hosts the genome and is essential for vital processes like DNA replication, DNA repair, transcription, and splicing. The genome is non-randomly organized in the three-dimensional space of the nucleus. This functional sub-compartmentalization was thought to be organized on the framework of nuclear matrix (NuMat), a non-chromatin scaffold that functions as a s...... hiện toàn bộ