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Studies on cell division in mammalian cells: VI. A temperature-sensitive mutant blocked in both G1 and G2 phases of the cell cycle
Springer Science and Business Media LLC - Tập 8 - Trang 653-666 - 1982
A temperature-sensitive mammalian cell cycle mutant with blocks in G
1 and G2 phases of the cell cycle has been isolated in culture. When shifted from the permissive temperature of 33‡ C to the nonpermissive temperature of 39‡ C, the fraction of cells initiating DNA synthesis as well as the fraction of cells entering mitosis decreased rapidly. Combined cytophotometric and autoradiographic analysi......
Fast-twitch and slow-twitch/cardiac Ca2+ ATPase genes map to human chromosomes 16 and 12
Springer Science and Business Media LLC - Tập 14 - Trang 645-645 - 1988
Increased intracellular phosphoribosylpyrophosphate and accelerated orotic acid decarboxylation in a mouse cell line resistant to purine and pyrimidine ribonucleosides
Springer Science and Business Media LLC - Tập 3 - Trang 263-280 - 1977
A line of mouse fibroblasts (A9AU-1), originally selected for growth in the presence of 6-azauridine, has been found to be resistant to cytotoxic concentrations of adenosine, guanosine, and thymidine. A9AU-1 cells convert orotic acid to uridine 5′-monophosphate at twice the rate of the A9P line from which the A9AU-1 clone was selected. The resistant cells also excrete purines, synthesized de novo,......
Toward Expression Mapping of Albinism-Deafness Syndrome (ADFN) Locus on Chromosome Xq26
Springer Science and Business Media LLC - Tập 24 - Trang 135-140 - 1998
We have employed a direct cDNA selection methodology to isolate transcribed sequences encoded in the human chromosomal interval Xq26 that contains the gene for X-chromosome linked albinism deafness syndrome (ADFN). ADFN had been previously mapped to an 8 centi Morgan region on chromosome Xq26. We have constructed six cDNA libraries specific to six YACs mapping to a 1.5 mb span at ......
The Control of cell proliferation by preformed purines: A genetic study I. Isolation and preliminary characterization of Chinese hamster lines with single or multiple defects in purine “salvage” pathways
Springer Science and Business Media LLC - Tập 3 - Trang 497-511 - 1977
Sublines with single or multiple defects in purine “salvage” enzymes were isolated from the Chinese hamster fibroblastic line GMA32 through single or successive onestep selections for resistance to purine analogs. They were examined for their ability to incorporate purine bases and nucleosides into macromolecules, for their sensitivity to growth inhibitory purines, and for their rescue by exogeno......
Modulation ofAPRT transcription by altering spacing betweenCis-regulatory elements
Springer Science and Business Media LLC - Tập 21 - Trang 43-50 - 1995
Three regulatory regions on the promoter of CHO adenine phosphoribosyltransferase gene (APRT) were identified. Spacing constraints between these regions were analyzed. With normal spacing, region II (−33 to +19), which separates regions I (−101 to −53) and III (+56 to +85), is critical forAPRT transcription. However, when regions I and III are artificially placed in proximity to each other (region......
Gene transfer into rat airway epithelial cells using retroviral vectors
Springer Science and Business Media LLC - Tập 17 - Trang 185-190 - 1991
Primary cultures of epithelial cells from adult rat tracheas were maintained in vitro on collagen matrices and were exposed to a murine retrovirus vector expressing theE. coli β-galactosidase gene. Infection was carried out on cells grown as monolayers under medium and on cells grown on raised platforms. Cells maintained at an air-medium interface were highly susceptible to infection with the vect......
Genetic analysis of resistance to total bromodeoxyuridine substitution in mammalian cell hybrids
Springer Science and Business Media LLC - Tập 17 - Trang 567-572 - 1991
Somatic cell hybrids derived from the fusion of Chinese hamster ovary cells (CHO) and mutant Syrian hamster melanoma cells (2E) were tested for their ability to grow with all of the thymidine (dThd) in their DNA replaced with 5-bromo-2′-deoxyuridine (BrdU), a phenotypic capability of the 2E cells but not of the CHO cells. Under these conditions, the 2E cells survived and grew, all of the hybrid cl......
Three distinct loci on human chromosome 21 contribute to interferon-α/β responsiveness
Springer Science and Business Media LLC - Tập 21 - Trang 139-145 - 1995
The species specificity of interferons (IFNs) depends on restricted recognition of these ligands by multisubunit cell surface receptors. Expression of the human receptor subunit IFNAR in mouse cells conferred sensitivity only to one subtype of human IFN, IFN-αB. Other genes on human chromosome 21 were required for responses to other subtypes of type I IFN. In contrast, IFNAR expression in hamster ......
Complementing mutant alleles define three loci involved in mannosylation of Man5-GlcNAc2-P-P-dolichol in Chinese hamster ovary cells
Springer Science and Business Media LLC - Tập 16 - Trang 539-548 - 1990
Dolichol-linked oligosaccharides consisting of twoN-acetylglucosamine, nine mannose, and three glucose residues (Glc3Man9GlcNAc2) are transferred to proteins that contain the consensus sequence Asn-X-Ser/Thr. This transfer occurs upon protein import into the lumen of the endoplasmic reticulum. An intermediate in the biosynthesis of the Glc3Man9GlcNAc2 lipid-linked oligosaccharide contains two GlcN......
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