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MicroRNA biogenesis and their functions in regulating stem cell potency and differentiation
Springer Science and Business Media LLC - - 2016
Stem cells are unspecialized/undifferentiated cells that exist in embryos and adult tissues or can be converted from somatic differentiated cells. Use of stem cells for tissue regeneration and tissue engineering has been a cornerstone of the regenerative medicine. Stem cells are also believed to exist in cancer tissues, namely cancer stem cells (CSCs). Growing evidence suggests that CSCs are the c...... hiện toàn bộ
Programmable Biosensors Based on RNA-Guided CRISPR/Cas EndonucleaseAbstract Highly infectious illnesses caused by pathogens constitute severe threats to public health and lead to global economic loss. The use of robust and programmable clustered regularly interspaced short palindromic repeat and CRISPR-associated protein (CRISPR-Cas) systems, repurposed from genome-engineering applications has markedly improved traditional nucleic ... ... hiện toàn bộ
Springer Science and Business Media LLC -
A quantitative PCR method for measuring absolute telomere length
Springer Science and Business Media LLC - Tập 13 - Trang 1-10 - 2011
We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative measurement of telomere length (TL) but modified by introducing an oligomer standard to measure aTL. The method describes the oligomer standards, the generation of the standard curve and the cal...... hiện toàn bộ
An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus
Springer Science and Business Media LLC - Tập 18 - Trang 1-9 - 2016
A number of integrase defective lentiviral (IDLV) packaging systems have been developed to produce integration deficient lentiviruses for gene delivery and epichromosomal expression. However, despite their growing demand, a comparative study to systemically evaluate the performance efficiency of different mutants on virus packaging and gene expression has not been done. Site-directed mutagenesis w...... hiện toàn bộ
A novel method to analyze leukocyte rolling behavior in vivo
Springer Science and Business Media LLC - Tập 6 Số 1 - Trang 173-179 - 2004
Use of RNA Immunoprecipitation Method for Determining Sinorhizobium meliloti RNA-Hfq Protein Associations In Vivo
Springer Science and Business Media LLC - Tập 20 - Trang 1-8 - 2018
Soil bacterium Sinorhizobium meliloti (S. meliloti) forms an endosymbiotic partnership with Medicago truncatula (M. truncatula) roots which results in root nodules. The bacteria live within root nodules where they function to fix atmospheric N2 and supply the host plant with reduced nitrogen. The bacterial RNA-binding protein Hfq (Hfq) is an important regulator for the effectiveness of the nitroge...... hiện toàn bộ
A Practical Guide to Rodent Islet Isolation and Assessment
Springer Science and Business Media LLC - - 2009
Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, p...... hiện toàn bộ
A BioBrick™-Compatible Vector for Allelic Replacement Using the XylE Gene as Selection Marker
Springer Science and Business Media LLC - Tập 18 - Trang 1-5 - 2016
Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods include high false positive rates due to mutations in counter-selection genes, and limited applicability to specific strains or growth media. Finally, solutions compatible with physical standar...... hiện toàn bộ
A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide
Springer Science and Business Media LLC - Tập 5 - Trang 53-62 - 2003
Excitable cells in many endocrine and neuronal systems display rhythms with periodicities on the order of many minutes. To observe firing patterns that represent the output of these rhythms requires a recording technique that can monitor electrophysiological activity for several hours without affecting cell behavior. A targeted extracellular approach (also known as loose-patch) accomplishes this o...... hiện toàn bộ
Mối tương quan giữa các thông số định lượng của siêu âm tăng cường tương phản và mô phỏng mạch máu trong mô hình khối u chuột: một kỹ thuật không xâm lấn mới để đánh giá? Dịch bởi AI
Springer Science and Business Media LLC - Tập 21 - Trang 1-8 - 2019
Mô phỏng mạch máu (VM) là một cơ chế mới cung cấp máu cho khối u khác biệt với mạch máu nội mô (EV). VM có liên quan đến sự ác tính, xâm lấn, di căn và tiên lượng kém. Đến nay, vẫn chưa có phương pháp không xâm lấn nào để đánh giá VM in vivo. Siêu âm tăng cường tương phản (CEUS) đã được thực hiện để đánh giá các thông số định lượng của khối u ở chuột. Nhuộm CD31 bằng hóa mô miễn dịch - phản ứng đồ...... hiện toàn bộ
#Mô phỏng mạch máu #siêu âm tăng cường tương phản #khối u #mật độ mạch máu #phương pháp không xâm lấn.
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