Research in Experimental Medicine

The alterations of the plasma level of elastase 1 (Ela) were investigated in 15 patients undergoing total pancreatectomy (TP), 18 patients undergoing pancreatoduodenectomy (PD), 8 patients undergoing distal pancreatectomy (DP) and 10 patients undergoing gastrectomy for gastric cancer (GR). The Ela level decreased rapidly below the normal level after TP. The plasma half-life of Ela was 4.6±1.2 h (Mean±SEM). The Ela levels after PD were abnormally elevated until the 7th postoperative day. The Ela levels after DP and GR were elevated gradually until the 7th postoperative day. From 1 to 6 months after PD, DP and GR, the Ela levels were normal. An additional release of Ela from the remaining pancreas following direct surgical interventions to the pancreas resulted in the elevated of the plasma level of Ela in the early postoperative period. The plasma level of Ela was normalized within 2 weeks after pancreatic surgery.

The unidirectional ileal transport of calcium (Ca) and inorganic phosphorus (Pi) of rats was measured in vitro with the modified Ussing technique. Animal preparation included 5/6 nephrectomy and EHDP treatment. They were compared to controls as well as to 1,25 (OH)2D3 supplemented rats. The results show that the ileum is a secretory organ for Ca and Pi, the serosa to mucosa transport (Jsm) exceeds the mucosa to serosa transport (Jms). Ca and Pi transport in sm direction is 1,25 (OH)2D3 independent but exhibits a mutual strong correlation. Our observations together with published data are in favor of mainly paracellular, non electrogenic sm transport of both ions. However, the factor controlling sm transport of Ca and Pi remains unidentified. The mucosa to serosa transport (Jms) in the ileum is low for both ions. Ca ms is stimulated by 1,25 (OH)2D3, Pi ms is unchanged.

The importance of the point of removal for the ingrowth quota of autologous spongiosa was examined in seven sheep. In six sheep spongiosa was taken from five different regions (proximal radius, olecranon, pelvic crest, trochanter major, and proximal tibia) and was transplanted autologously. Defined corticalis defects of the tibia were the chosen sites for the heterotopic implantations. The ingrowth quota were tested by X-ray as well as scintigraphically, histologically, and fluorescent-microscopically; one animal was taken for control. A judgement was made by quantifying scintigraphy and fluorescent-microscopic histometry. Better ingrowth quota were found for spongiosa from the pelvic crest, proximal tibia, and trochanter major as compared to qualitatively clearly worse transplants from radius base and olecranon. The results from the animal experiments prove the clinical experience.

1. Die Reihenfolge im Auftreten der Komponenten der periodischen Tätigkeit des Verdauungskanals ist bei demselben Hund keine beständige. 2. Es werden Fälle von zeitlichem Auseinandergehen der Komponenten und von Herausfallen von einigen Komponenten beobachtet. 3. Die Magenarbeitsperioden können mittels kurz dauernder Hemmung zerspaltet werden. Die nach Hemmungsabschluß wieder hergestellten Magenbewegungen beendigen aber die „Arbeitsperiode“ einfach algebraisch nicht. Sie können in verschiedenartigen Beziehungen sowohl zu den vorangehenden Kontraktionen, wie auch zu der nächstfolgenden Arbeitsperiode stehen. 4. Die beschriebenen Eigentümlichkeiten im Auftreten der Komponenten können in Zusammenhang mit experimentellen Befunden bezüglich der periodischen Erregbarkeitsveränderungen der Verdauungsapparate erklärt werden. 5. Die periodischen Erscheinungen entfalten sich nicht „plötzlich“, sondern allmählich. Relative „Plötzlichkeit“ kommt nur den groben Äußerungen der periodischen Tätigkeit zu. 6. Der Verlauf der periodischen Tätigkeit wird durch gegenseitige hemmende und exzitorische Einflüsse seitens verschiedener Verdauungsapparate kompliziert.