Protein Science

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De novo design of the hydrophobic cores of proteins
Protein Science - Tập 4 Số 10 - Trang 2006-2018 - 1995
John R. Desjarlais, Tracy M. Handel
AbstractWe have developed and experimentally tested a novel computational approach for the de novo design of hydrophobic cores. A pair of computer programs has been written, the first of which creates a “custom” rotamer library for potential hydrophobic residues, based on the backbone structure of the protein of interest. The second program uses a genetic algorithm...... hiện toàn bộ
The structure of human pancreaticα-amylase at 1.8 Å resolution and comparisons with related enzymes
Protein Science - Tập 4 Số 9 - Trang 1730-1742 - 1995
Gary D. Brayer, Yaoguang Luo, Stephen G. Withers
Carbohydrate binding sites in a pancreatic α‐amylase‐substrate complex, derived from X‐ray structure analysis at 2.1 Å resolution
Protein Science - Tập 4 Số 4 - Trang 747-755 - 1995
Minxie Qian, Richard Haser, F. Payan
AbstractThe X‐ray structure analysis of a crystal of pig pancreatic α‐amylase (PPA, EC 3.2.1.1.) that was soaked with the substrate maltopentaose showed electron density corresponding to two independent carbohydrate recognition sites on the surface of the molecule. Both binding sites are distinct from the active site described in detail in our previous high‐resolut...... hiện toàn bộ
Outer membrane protein A of E. coli folds into detergent micelles, but not in the presence of monomeric detergent
Protein Science - Tập 8 Số 10 - Trang 2065-2071 - 1999
Jörg H. Kleinschmidt, Michael C. Wiener, Lukas K. Tamm
AbstractOuter membrane protein A (OmpA) of Escherichia coli is a β‐barrel membrane protein that unfolds in 8 M urea to a random coil. OmpA refolds upon urea dilution in the presence of certain detergents or lipids. To examine the minimal requirements for secondary and tertiary structure formation in β‐barrel membrane proteins, folding of ...... hiện toàn bộ
Protein structure refinement based on paramagnetic NMR shifts: Applications to wild‐type and mutant forms of cytochrome c
Protein Science - Tập 4 Số 2 - Trang 296-305 - 1995
Miriam Gochin, Heinrich Röder
AbstractA new approach to NMR solution structure refinement is introduced that uses paramagnetic effects on nuclear chemical shifts as constraints in energy minimization or molecular dynamics calculations. Chemical shift differences between oxidized and reduced forms of horse cytochrome c for more than 300 protons were used as constraints...... hiện toàn bộ
The 1.9 Å crystal structure of Escherichia coli MurG, a membrane‐associated glycosyltransferase involved in peptidoglycan biosynthesis
Protein Science - Tập 9 Số 6 - Trang 1045-1052 - 2000
Sha Ha, David Walker, Yigong Shi, Suzanne Walker
AbstractThe 1.9 Å X‐ray structure of a membrane‐associated glycosyltransferase involved in peptidoglycan biosynthesis is reported. This enzyme, MurG, contains two α/β open sheet domains separated by a deep cleft. Structural analysis suggests that the C‐terminal domain contains the UDP‐GlcNAc binding site while the N‐terminal domain contains the acceptor binding sit...... hiện toàn bộ
Crystal structure of brain‐type creatine kinase at 1.41 Å resolution
Protein Science - Tập 8 Số 11 - Trang 2258-2269 - 1999
Michael Eder, Uwe Schlattner, Theo Wallimann, Andreas Becker, Wolfgang Kabsch, Karin Fritz‐Wolf
AbstractExcitable cells and tissues like muscle or brain show a highly fluctuating consumption of ATP, which is efficiently regenerated from a large pool of phosphocreatine by the enzyme creatine kinase (CK). The enzyme exists in tissue–as well as compartment‐specific isoforms. Numerous pathologies are related to the CK system: CK is found to be overexpressed in a ...... hiện toàn bộ
Modulation of antibody affinity by a non‐contact residue
Protein Science - Tập 2 Số 2 - Trang 206-214 - 1993
Joel F. Schildbach, Richard I. Near, Robert E. Bruccoleri, Edgar Haber, P.D. Jeffrey, Jiřı́ Novotný, S. Sheriff, Michael N. Margolies
AbstractAntibody LB4, produced by a spontaneous variant of the murine anti‐digoxin monoclonal antibody 26–10, has an affinity for digoxin two orders of magnitude lower than that of the parent antibody due to replacement of serine with phenylalanine at position 52 of the heavy chain variable region (Schildbach, J.F., Panka, D.J., Parks, D.R., et al., 1991, ... hiện toàn bộ
A ligand-induced conformational change in theyersiniaprotein tyrosine phosphatase
Protein Science - Tập 4 Số 9 - Trang 1904-1913 - 1995
Heidi Schubert, Eric B. Fauman, Jeanne A. Stuckey, Jack E. Dixon, Mark A. Saper
High‐resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure‐based design of ribonucleolytic inhibitors
Protein Science - Tập 12 Số 11 - Trang 2559-2574 - 2003
D.D. Leonidas, G.B. Chavali, Nikos G. Oikonomakos, Evangelia D. Chrysina, Magda Kosmopoulou, Metaxia Vlassi, Claire Frankling, K. Ravi Acharya
AbstractThe crystal structures of bovine pancreatic ribonuclease A (RNase A) in complex with 3′,5′‐ADP, 2′,5′‐ADP, 5′‐ADP, U‐2′‐p and U‐3′‐p have been determined at high resolution. The structures reveal that each inhibitor binds differently in the RNase A active site by anchoring a phosphate group in subsite P1. The most potent inhibitor of al...... hiện toàn bộ
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