Molecular Microbiology

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Activation and silencing of leu‐500 promoter by transcription‐induced DNA supercoiling in the Salmonella chromosome
Molecular Microbiology - Tập 37 Số 3 - Trang 583-594 - 2000
Donia Hanafi, Lionello Bossi
The notion that transcription can generate supercoils in the DNA template largely stems from work with small circular plasmids. In the present work, we tested this model in the bacterial chromosome using a supercoiling‐sensitive promoter as a functional sensor of superhelicity changes. The leu‐500 promoter of Salmonella typhimurium is a...... hiện toàn bộ
NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility
Molecular Microbiology - Tập 73 Số 4 - Trang 680-694 - 2009
Jonathan D. Partridge, Diane Bodenmiller, Michael S. Humphrys, Stephen Spiro
SummaryThe Escherichia coli NsrR protein is a nitric oxide‐sensitive repressor of transcription. The NsrR‐binding site is predicted to comprise two copies of an 11 bp motif arranged as an inverted repeat with 1 bp spacing. By mutagenesis we confirmed that both 11 bp motifs are required for maximal NsrR repression of the ytfE<...... hiện toàn bộ
Proline 21, a residue within the α‐helical domain of ΦX174 lysis protein E, is required for its function in Escherichia coli
Molecular Microbiology - Tập 26 Số 2 - Trang 337-346 - 1997
Angela Witte, Gerald Schrot, Petra Schön, Werner Lubitz
ΦX174 lysis protein E‐mediated lysis of Escherichia coli is characterized by a protein E‐specific fusion of the inner and outer membrane and formation of a transmembrane tunnel structure. In order to understand the fusion process, the topology of protein E within the envelope complex of E. coli was investigated. Proteinase K protection ...... hiện toàn bộ
Export of active green fluorescent protein to the periplasm by the twin‐arginine translocase (Tat) pathway in Escherichia coli
Molecular Microbiology - Tập 39 Số 1 - Trang 47-53 - 2001
Joanna Smith, Richard A. Daniel, Jeff Errington, Colin Robinson
The twin‐arginine translocation (Tat) system targets cofactor‐containing proteins across the Escherichia coli cytoplasmic membrane via distinct signal peptides bearing a twin‐arginine motif. In this study, we have analysed the mechanism and capabilities of the E. coli Tat system using green fluorescent protein (GFP) fused to the twin‐ar...... hiện toàn bộ
Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent
Molecular Microbiology - Tập 5 Số 1 - Trang 117-122 - 1991
Axel A. Laminet, Carol A. Kumamoto, Andreas Plückthun
SummaryThe rate of folding of the precursor of β‐lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild‐type β‐lactamase and several mutants in the signal or the mature protein, affecting either transport or enzyme kinetics and probably folding, were examined for total expression, total enzymatic activity, a...... hiện toàn bộ
Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa
Molecular Microbiology - Tập 53 Số 3 - Trang 725-733 - 2004
Paul W. Denny, Deborah F. Smith
SummaryAlthough the concept of eukaryotic lipid rafts arouses controversy, recent biophysical studies strongly indicate that inducible, stable and relatively large rafts are a feature of activated mammalian cells. These studies allow us to consider the functional role of lipid rafts in kinetoplastid parasites, which are particularly rich in lipid‐anchored surface m...... hiện toàn bộ
Limited tolerance towards folded elements during secretion of the autotransporter Hbp
Molecular Microbiology - Tập 63 Số 5 - Trang 1524-1536 - 2007
Wouter S. P. Jong, Corinne M. ten Hagen‐Jongman, Tanneke den Blaauwen, Dirk Jan Slotboom, Jeremy R. H. Tame, David Wickström, Jan‐Willem de Gier, Ben R. Otto, Joen Luirink
SummaryMany virulence factors secreted by pathogenic Gram‐negative bacteria belong to the autotransporter (AT) family. ATs consist of a passenger domain, which is the actual secreted moiety, and a β‐domain that facilitates the transfer of the passenger domain across the outer membrane. Here, we analysed folding and translocation of the AT passenger, using ... hiện toàn bộ
Iron‐sulphur clusters and the problem with oxygen
Molecular Microbiology - Tập 59 Số 4 - Trang 1073-1082 - 2006
James A. Imlay
SummaryDuring the first billion years of life on the Earth, the environment was anaerobic. Iron and sulphur were plentiful, and they were recruited in the formation of iron‐sulphur (Fe‐S) clusters within ancient proteins. These clusters provided many enzymes with the ability to transfer electrons; to others they offered a cationic feature that tightly bound oxyanio...... hiện toàn bộ
Importance of using lac rather than ara promoter vectors for modulating the levels of toxic gene products in Escherichia coli
Molecular Microbiology - Tập 30 Số 3 - Trang 676-678 - 1998
Lida Hashemzadeh‐Bonehi, Farideh Mehraein‐Ghomi, Costas Mitsopoulos, Jansen Jacob, Emma S. Hennessey, Jenny K. Broome‐Smith
Missense mutations in the catalase‐peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis
Molecular Microbiology - Tập 15 Số 2 - Trang 235-245 - 1995
Béate Heym, Pedro M. Alzari, Nadine Honoré, Stewart T. Cole
SummaryThe toxicity of the powerful anti‐tuberculosis drug isoniazid (INH) is believed to be mediated by the haem‐containing enzyme catalase‐peroxidase, encoded by the katG gene of Mycobacterium tuberculosis. Compelling evidence for this was obtained by studying a panel of INH‐resistant clinical isolates using a...... hiện toàn bộ
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