Molecular Microbiology
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NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motilitySummary The Escherichia coli NsrR protein is a nitric oxide‐sensitive repressor of transcription. The NsrR‐binding site is predicted to comprise two copies of an 11 bp motif arranged as an inverted repeat with 1 bp spacing. By mutagenesis we confirmed that both 11 bp motifs are required for maximal NsrR repression of the ytfE<... ...
Molecular Microbiology - Tập 73 Số 4 - Trang 680-694 - 2009
Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoaSummary Although the concept of eukaryotic lipid rafts arouses controversy, recent biophysical studies strongly indicate that inducible, stable and relatively large rafts are a feature of activated mammalian cells. These studies allow us to consider the functional role of lipid rafts in kinetoplastid parasites, which are particularly rich in lipid‐anchored surface m... ...
Molecular Microbiology - Tập 53 Số 3 - Trang 725-733 - 2004
Limited tolerance towards folded elements during secretion of the autotransporter HbpSummary Many virulence factors secreted by pathogenic Gram‐negative bacteria belong to the autotransporter (AT) family. ATs consist of a passenger domain, which is the actual secreted moiety, and a β‐domain that facilitates the transfer of the passenger domain across the outer membrane. Here, we analysed folding and translocation of the AT passenger, using ...
Molecular Microbiology - Tập 63 Số 5 - Trang 1524-1536 - 2007
Iron‐sulphur clusters and the problem with oxygenSummary During the first billion years of life on the Earth, the environment was anaerobic. Iron and sulphur were plentiful, and they were recruited in the formation of iron‐sulphur (Fe‐S) clusters within ancient proteins. These clusters provided many enzymes with the ability to transfer electrons; to others they offered a cationic feature that tightly bound oxyanio... ...
Molecular Microbiology - Tập 59 Số 4 - Trang 1073-1082 - 2006
Mycobacterium tuberculosis is a natural mutant with an inactivated oxidative‐stress regulatory gene:implications for sensitivity to isoniazidThe systems participating in detoxification of reactive oxygen intermediates in Mycobacterium tuberculosis are believed to play a dual role in the biology of this highly adapted human pathogen: (i) they may contribute to the survival of this bacterium in the host; and (ii) alterations in the gene encoding catalase/peroxidase have been linked to this organism's re... ...
Molecular Microbiology - Tập 17 Số 5 - Trang 889-900 - 1995
ahpC, a gene involved in isoniazid resistance of the Mycobacterium tuberculosis complexA gene conferring low‐level isoniazid (INH) resistance on Mycobacterium smegmatis was isolated from a cosmid library of the genome of an INH‐resistant Mycobacterium bovis strain. The gene had good homology with ahpC , the product of which is a subunit of alkyl hydroperoxide reductase, and also with a family of ... ...
Molecular Microbiology - Tập 19 Số 5 - Trang 1025-1034 - 1996
Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosolCells exposed to Helicobacter pylori toxin VacA develop large vacuoles that originate from massive swelling of membranous compartments of late stages of the endocytic pathway. To determine if the toxin is active from the cell cytosol, cells were either microinjected with toxin or transfected with plasmids encoding VacA. Both procedures cause formation of intracel... ...
Molecular Microbiology - Tập 26 Số 4 - Trang 665-674 - 1997
Identification of a PEST‐like motif in listeriolysin O required for phagosomal escape and for virulence in Listeria monocytogenesThe hly ‐encoded listeriolysin O (LLO) is a major virulence factor secreted by the intracellular pathogen Listeria monocytogenes , which plays a crucial role in the escape of bacteria from the phagosomal compartment. Here, we identify a putative PEST sequence close to the N‐terminus of LLO and focus on the role of this motif in the biolog... ...
Molecular Microbiology - Tập 39 Số 5 - Trang 1124-1139 - 2001
WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A‐coreSummary waaL has been implicated as the gene that encodes the O‐antigen ligase. To date, in vitro biochemical evidence to prove that WaaL possesses ligase activity has been lacking due to the difficulty of purifying WaaL and unavailability of substrates. Here we describe the purification of WaaL, a membrane prot... ...
Molecular Microbiology - Tập 65 Số 5 - Trang 1345-1359 - 2007
Characterization and distribution of two insertion sequences, IS1191 and iso‐IS981, in Streptococcus thermophilus: does intergeneric transfer of insertion sequences occur in lactic acid bacteria co‐cultures?Summary A chromosomal repeated sequence from Streptococcus thermophilus was identified as a new insertion sequence (IS), IS1191. This is the first IS element characterized in this species. This 1313 bp element has 28 bp imperfect terminal inverted repeats and is flanked by short direct repeats of 8bp. The single ... ...
Molecular Microbiology - Tập 16 Số 1 - Trang 69-78 - 1995
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