Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independentMolecular Microbiology - Tập 5 Số 1 - Trang 117-122 - 1991
Axel A. Laminet, Carol A. Kumamoto, Andreas Plückthun
SummaryThe rate of folding of the precursor of β‐lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild‐type β‐lactamase and several mutants in the signal or the mature protein, affecting either transport or enzyme kinetics and probably folding, were examined for total expression, total enzymatic activity, a...... hiện toàn bộ
Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoaMolecular Microbiology - Tập 53 Số 3 - Trang 725-733 - 2004
Paul W. Denny, Deborah F. Smith
SummaryAlthough the concept of eukaryotic lipid rafts arouses controversy, recent biophysical studies strongly indicate that inducible, stable and relatively large rafts are a feature of activated mammalian cells. These studies allow us to consider the functional role of lipid rafts in kinetoplastid parasites, which are particularly rich in lipid‐anchored surface m...... hiện toàn bộ
Limited tolerance towards folded elements during secretion of the autotransporter HbpMolecular Microbiology - Tập 63 Số 5 - Trang 1524-1536 - 2007
Wouter S. P. Jong, Corinne M. ten Hagen‐Jongman, Tanneke den Blaauwen, Dirk Jan Slotboom, Jeremy R. H. Tame, David Wickström, Jan‐Willem de Gier, Ben R. Otto, Joen Luirink
SummaryMany virulence factors secreted by pathogenic Gram‐negative bacteria belong to the autotransporter (AT) family. ATs consist of a passenger domain, which is the actual secreted moiety, and a β‐domain that facilitates the transfer of the passenger domain across the outer membrane. Here, we analysed folding and translocation of the AT passenger, using ... hiện toàn bộ
Iron‐sulphur clusters and the problem with oxygenMolecular Microbiology - Tập 59 Số 4 - Trang 1073-1082 - 2006
James A. Imlay
SummaryDuring the first billion years of life on the Earth, the environment was anaerobic. Iron and sulphur were plentiful, and they were recruited in the formation of iron‐sulphur (Fe‐S) clusters within ancient proteins. These clusters provided many enzymes with the ability to transfer electrons; to others they offered a cationic feature that tightly bound oxyanio...... hiện toàn bộ