Molecular Microbiology
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Activation and silencing of leu‐500 promoter by transcription‐induced DNA supercoiling in the Salmonella chromosome
Molecular Microbiology - Tập 37 Số 3 - Trang 583-594 - 2000
The notion that transcription can generate supercoils in the DNA template
largely stems from work with small circular plasmids. In the present work, we
tested this model in the bacterial chromosome using a supercoiling‐sensitive
promoter as a functional sensor of superhelicity changes. The leu‐500 promoter
of Salmonella typhimurium is a mutant and inactive variant of the leucine operon
promoter th...
NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility
Molecular Microbiology - Tập 73 Số 4 - Trang 680-694 - 2009
SummaryThe Escherichia coli NsrR protein is a nitric oxide‐sensitive repressor
of transcription. The NsrR‐binding site is predicted to comprise two copies of
an 11 bp motif arranged as an inverted repeat with 1 bp spacing. By mutagenesis
we confirmed that both 11 bp motifs are required for maximal NsrR repression of
the ytfE promoter. We used chromatin immunoprecipitation and microarray analysis
(...
Proline 21, a residue within the α‐helical domain of ΦX174 lysis protein E, is required for its function in Escherichia coli
Molecular Microbiology - Tập 26 Số 2 - Trang 337-346 - 1997
ΦX174 lysis protein E‐mediated lysis of Escherichia coli is characterized by a
protein E‐specific fusion of the inner and outer membrane and formation of a
transmembrane tunnel structure. In order to understand the fusion process, the
topology of protein E within the envelope complex of E. coli was investigated.
Proteinase K protection studies showed that, during the time course of protein
E‐media...
Export of active green fluorescent protein to the periplasm by the twin‐arginine translocase (Tat) pathway in Escherichia coli
Molecular Microbiology - Tập 39 Số 1 - Trang 47-53 - 2001
The twin‐arginine translocation (Tat) system targets cofactor‐containing
proteins across the Escherichia coli cytoplasmic membrane via distinct signal
peptides bearing a twin‐arginine motif. In this study, we have analysed the
mechanism and capabilities of the E. coli Tat system using green fluorescent
protein (GFP) fused to the twin‐arginine signal peptide of TMAO reductase
(TorA). Fractionation ...
Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent
Molecular Microbiology - Tập 5 Số 1 - Trang 117-122 - 1991
SummaryThe rate of folding of the precursor of β‐lactamase is not influenced by
the presence of SecB under conditions in which GroEL/ES retards the folding.
Wild‐type β‐lactamase and several mutants in the signal or the mature protein,
affecting either transport or enzyme kinetics and probably folding, were
examined for total expression, total enzymatic activity, and transported
β‐lactamase (in vi...
Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa
Molecular Microbiology - Tập 53 Số 3 - Trang 725-733 - 2004
SummaryAlthough the concept of eukaryotic lipid rafts arouses controversy,
recent biophysical studies strongly indicate that inducible, stable and
relatively large rafts are a feature of activated mammalian cells. These studies
allow us to consider the functional role of lipid rafts in kinetoplastid
parasites, which are particularly rich in lipid‐anchored surface molecules.
Morphological, biochemi...
Limited tolerance towards folded elements during secretion of the autotransporter Hbp
Molecular Microbiology - Tập 63 Số 5 - Trang 1524-1536 - 2007
SummaryMany virulence factors secreted by pathogenic Gram‐negative bacteria
belong to the autotransporter (AT) family. ATs consist of a passenger domain,
which is the actual secreted moiety, and a β‐domain that facilitates the
transfer of the passenger domain across the outer membrane. Here, we analysed
folding and translocation of the AT passenger, using Escherichia coli
haemoglobin protease (Hbp...
Iron‐sulphur clusters and the problem with oxygen
Molecular Microbiology - Tập 59 Số 4 - Trang 1073-1082 - 2006
SummaryDuring the first billion years of life on the Earth, the environment was
anaerobic. Iron and sulphur were plentiful, and they were recruited in the
formation of iron‐sulphur (Fe‐S) clusters within ancient proteins. These
clusters provided many enzymes with the ability to transfer electrons; to others
they offered a cationic feature that tightly bound oxyanionic and nitrogenous
metabolites. ...
Importance of using lac rather than ara promoter vectors for modulating the levels of toxic gene products in Escherichia coli
Molecular Microbiology - Tập 30 Số 3 - Trang 676-678 - 1998
Missense mutations in the catalase‐peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis
Molecular Microbiology - Tập 15 Số 2 - Trang 235-245 - 1995
SummaryThe toxicity of the powerful anti‐tuberculosis drug isoniazid (INH) is
believed to be mediated by the haem‐containing enzyme catalase‐peroxidase,
encoded by the katG gene of Mycobacterium tuberculosis. Compelling evidence for
this was obtained by studying a panel of INH‐resistant clinical isolates using a
novel strategy based on the polymerase chain reaction and
single‐strand‐conformation p...
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