Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent

Molecular Microbiology - Tập 5 Số 1 - Trang 117-122 - 1991
Axel A. Laminet1, Carol A. Kumamoto2, Andreas Plückthun1
1Genzentrum der Universität München, Max-Planck-Institut für Biochemie, D-8033 Martinsried, Germany.
2Departments of Physiology, and Molecular Biology and Microbiology. Tufts University School of Medicine, 136 Harrison Avenue, Boston. Massachusetts 02111. USA.

Tóm tắt

SummaryThe rate of folding of the precursor of β‐lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild‐type β‐lactamase and several mutants in the signal or the mature protein, affecting either transport or enzyme kinetics and probably folding, were examined for total expression, total enzymatic activity, and transported β‐lactamase (in vivo resistance) in secB and secB+ strains. We conclude that there is no indication of any relevant interaction between SecB and pre‐β‐lactamase in vitro, nor did the secB mutation affect the transport of wild–type β‐lactamase or any of the mutants in vivo. Thus, putative Escherichia coli‘folding modulators’must be of limited specificity.

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