Methods in Cell Science
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The laboratory of Alexis Carrel at the Rockefeller institute
Methods in Cell Science - Tập 1 - Trang 1-2 - 1975
Electroporation as a technique for the transfer of macromolecules into mammalian cell lines
Methods in Cell Science - Tập 15 - Trang 56-62 - 1993
Numerous methods have been devised to facilitate the introduction of exogenous compounds into cells. The technique of electroporation allows the direct physical transfer of numerous kinds of molecules into essentially any cell, but the major application has been for transfection of DNA and this emphasis is recapitulated here. However, the conditions for transfer of DNA or other macromolecules are ...... hiện toàn bộ
Electron microscopy of endothelial cells in culture: II. Scanning electron microscopy and OTOTO impregnation method
Methods in Cell Science - Tập 10 - Trang 35-36 - 1986
A method is described whereby endothelial cells are treated with several cycles of osmium and thiocarbohydrazide then critical point dried. Cells grown on Formvar-coated gold grids can be examined directly by transmission electron microscopy, cells grown on glass strips are mounted on stubs for scanning electron microscopy and are scanned without further coating with gold or palladium.
A procedure for the serum-free growth of normal human diploid fibroblasts
Methods in Cell Science - Tập 6 - Trang 123-126 - 1980
We have developed a method for the growth of WI-38 cells in a serum-free medium. Basal medium MCDB-104 is supplemented with platelet-derived growth factor (30 µg/ml), epidermal growth factor (100 ng/ml), insulin (5 µg/ml), transferrin (5 µg/ml), and dexamethasone (55 ng/ml). With this medium cells will grow at a rate and to an extent similar to that produced by medium containing 10% serum. During ...... hiện toàn bộ
Cell cycle synchronizing properties of staurosporine
Methods in Cell Science - Tập 18 - Trang 99-107 - 1996
Staurosporine (SSP) is a microbial alkaloid isolated from cultures of Streptomyces. For its ability to specifically inhibit protein kinase C and other serine/threonine and tyrosine protein kinases, SSP has been utilized recently to synchronize cells in G1 and/or G2 phases of the cell cycle. In the present paper we focus on the synchronizing properties of SSP with respect to three human normal cell...... hiện toàn bộ
Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo
Methods in Cell Science - Tập 25 Số 1/2 - Trang 59-64 - 2003
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