Richard G. Hansford, Barbara A. Hogue, Vida Mildaziene
We have examined the substrate specificity and inhibitor sensitivity of H2O2
formation by rat heart mitochondria. Active H2O2 production requires both a high
fractional reduction of Complex I (indexed by NADH/NAD+ + NADH ratio) and a high
membrane potential, ΔΨ. These conditions are achieved with supraphysiological
concentrations of succinate. With physiological concentrations of NAD-linked
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