Generation and Characterization of Anti-MUC4 Monoclonal Antibodies Reactive with Normal and Cancer Cells in HumansJournal of Histochemistry and Cytochemistry - Tập 52 Số 2 - Trang 253-261 - 2004
Nicolas Moniaux, Grish C. Varshney, Subhash C. Chauhan, M.C. Copin, Maneesh Jain, Uwe A. Wittel, Mahefatiana Andrianifahanana, Isabelle Van Seuningen, Surinder K. Batra
We have previously cloned the full-length cDNA (approximately 28 Kb) and established the complete genomic organization (25 exons/introns over 100 kb) of the human MUC4 mucin. This large molecule is predicted to protrude over 2 μm above the cell surface, in which MUC4α is an extracellular mucin-type glycoprotein subunit and MUC4β is the transmembrane subunit. Over two thirds of the encoded...... hiện toàn bộ
THE SITE OF FERRICYANIDE REDUCTION BY REDUCTASES WITHIN MITOCHONDRIA AS STUDIED BY ELECTRON MICROSCOPYJournal of Histochemistry and Cytochemistry - Tập 16 Số 1 - Trang 49-57 - 1968
Kazuo Ogawa, Takuma Saito, Hiroshi Mayahara
In the present investigation the ultrastructural site of ferricyanide reduction by reductases in mitochondria in tissues (heart and kidney) of the normal adult rat was studied by the copper ferrocyanide method developed recently in our laboratory. Substrates used were sodium succinate, cytochrome c, dihydronicotinamide-adenine dinucleotide (NADH2), sodium dl-β-hydroxy...... hiện toàn bộ
Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth FactorJournal of Histochemistry and Cytochemistry - Tập 45 Số 6 - Trang 795-804 - 1997
H. Francis Farhadi, Sangeeta Pareek, Robert Day, Weijia Dong, Michel Chrètien, John Bergeron, Nabil G. Seidah, Richard A. Murphy
Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from a 35-kD precursor by proteolytic enzymes that have yet to be identified. Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this study we questioned whether PCs could process salivary NGF in vivo. mRNA coding for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in sit...... hiện toàn bộ
Immunocytochemical localization of insulin- and glucagonlike peptides in rat salivary glands.Journal of Histochemistry and Cytochemistry - Tập 34 Số 5 - Trang 627-632 - 1986
Paul H. Smith, B. A. Toms
An avidin-biotin immunocytochemical technique was used to localize cells containing an insulin- or glucagon-like peptide in the major salivary glands of Sprague-Dawley rats. Cells with insulin-like staining were observed in the intercalated ducts of both the parotid and submandibular glands, but none were found in the sublingual gland. A discrete population of cells with intense glucagon-...... hiện toàn bộ
Involvement of Mesangial Cells Expressing α-Smooth Muscle Actin During Restorative Glomerular Remodeling in Thy-1.1 NephritisJournal of Histochemistry and Cytochemistry - Tập 54 Số 11 - Trang 1291-1301 - 2006
Koichiro Ichimura, Hidetake Kurihara, Tatsuo Sakai
The function of actin cytoskeleton in mesangial cells (MCs) during the recovering process of injured glomeruli is not fully understood. MCs in injured glomeruli express α-smooth muscle actin (α-SMA), which is not detected in normal glomeruli. We focused on the localization of α-SMA in MCs of Thy-1. 1 nephritic rat. Expression of α-SMA in the injured glomeruli peaked at day 5 after antibod...... hiện toàn bộ
Tensin Is Expressed in Glomerular Mesangial Cells and Is Related to Their Attachment to Surrounding Extracellular MatrixJournal of Histochemistry and Cytochemistry - Tập 52 Số 5 - Trang 683-691 - 2004
Hikaru Takahara, Isao Shirato, Katsuhiko Asanuma, M Yamashita, Yukihiko Takeda, Yasuhiko Tomino
Glomerular expression of tensin was immunohistochemically studied in normal and diseased rat kidneys to determine whether tensin might be related to specific binding in individual glomerular cells. Normal rat kidneys displayed an intense immunofluorescence reaction for tensin along the basal aspects of proximal and distal tubule cells and parietal epithelial cells of Bowman's capsules. In...... hiện toàn bộ