THE ROLE OF MICROSOMES IN THE INCORPORATION OF AMINO ACIDS INTO PROTEINSJournal of Histochemistry and Cytochemistry - Tập 2 Số 5 - Trang 378-386 - 1954
Elizabeth B. Keller, Paul C. Zamecnik, Robert B. Loftfield
These studies are an extension of previous findings of a relationship between
ribose nucleic acid and protein synthesis (Brachet, Caspersson, Borsook, Keller,
Siekevitz, Mirsky). In vivo experiments have been described which show that 70
per cent or more of the initial incorporation of leucine-C14 or valine-C14 in
the liver occurs in the protein of the microsome fraction, which contains most
of th... hiện toàn bộ
Generation and Characterization of Anti-MUC4 Monoclonal Antibodies Reactive with Normal and Cancer Cells in HumansJournal of Histochemistry and Cytochemistry - Tập 52 Số 2 - Trang 253-261 - 2004
Nicolas Moniaux, Grish C. Varshney, Subhash C. Chauhan, M.C. Copin, Maneesh Jain, Uwe A. Wittel, Mahefatiana Andrianifahanana, Isabelle Van Seuningen, Surinder K. Batra
We have previously cloned the full-length cDNA (approximately 28 Kb) and
established the complete genomic organization (25 exons/introns over 100 kb) of
the human MUC4 mucin. This large molecule is predicted to protrude over 2 μm
above the cell surface, in which MUC4α is an extracellular mucin-type
glycoprotein subunit and MUC4β is the transmembrane subunit. Over two thirds of
the encoded protein ... hiện toàn bộ
THE SITE OF FERRICYANIDE REDUCTION BY REDUCTASES WITHIN MITOCHONDRIA AS STUDIED BY ELECTRON MICROSCOPYJournal of Histochemistry and Cytochemistry - Tập 16 Số 1 - Trang 49-57 - 1968
Kazuo Ogawa, Takuma Saito, Hiroshi Mayahara
In the present investigation the ultrastructural site of ferricyanide reduction
by reductases in mitochondria in tissues (heart and kidney) of the normal adult
rat was studied by the copper ferrocyanide method developed recently in our
laboratory. Substrates used were sodium succinate, cytochrome c,
dihydronicotinamide-adenine dinucleotide (NADH2), sodium dl-β-hydroxybutyrate,
phenazine methosulfa... hiện toàn bộ
Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth FactorJournal of Histochemistry and Cytochemistry - Tập 45 Số 6 - Trang 795-804 - 1997
H. Francis Farhadi, Sangeeta Pareek, Robert Day, Weijia Dong, Michel Chrètien, John Bergeron, Nabil G. Seidah, Richard A. Murphy
Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from
a 35-kD precursor by proteolytic enzymes that have yet to be identified.
Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this
study we questioned whether PCs could process salivary NGF in vivo. mRNA coding
for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in
situ hybridi... hiện toàn bộ
Regulation of Na+,K+-ATPase in submandibular glands of hypophysectomized male mice by steroid and thyroid hormones.Journal of Histochemistry and Cytochemistry - Tập 44 Số 7 - Trang 703-711 - 1996
Kinji Kurihara, Shichiro Maruyama, Kazuo Hosoi, Seiichi Sato, T Ueha, Edward W. Gresik
The effects of thyroid hormone, androgen, glucocorticoid, and mineralocorticoid
on Na+,K+-ATPase activity and on levels of its alpha-subunit protein (alpha 1
isoform) in mouse submandibular gland (SMG) were studied by enzyme assay for
ouabain-sensitive ATP hydrolysis, by quantitative densitometric scanning of
Western blots, and by immunohistochemistry. To define the specific regulatory
effects of ... hiện toàn bộ
Immunocytochemical localization of insulin- and glucagonlike peptides in rat salivary glands.Journal of Histochemistry and Cytochemistry - Tập 34 Số 5 - Trang 627-632 - 1986
Paul H. Smith, B. A. Toms
An avidin-biotin immunocytochemical technique was used to localize cells
containing an insulin- or glucagon-like peptide in the major salivary glands of
Sprague-Dawley rats. Cells with insulin-like staining were observed in the
intercalated ducts of both the parotid and submandibular glands, but none were
found in the sublingual gland. A discrete population of cells with intense
glucagon-like immu... hiện toàn bộ