Journal of Histochemistry and Cytochemistry
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Actin Filament Organization of Foot Processes in Rat Podocytes The foot processes of podocytes possess abundant microfilaments and modulate glomerular filtration. We investigated the actin filament organization of foot processes in adult rat podocytes and the formation of the actin cytoskeletal system of immature podocytes during glomerulogenesis. Electron microscopy revealed two populations of actin cytoskeletons in foot processes of adult podocytes... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 51 Số 12 - Trang 1589-1600 - 2003
Simple and Rapid Methods for SEM Observation and TEM Immunolabeling of Rubber Particles We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving t... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 51 Số 8 - Trang 1105-1108 - 2003
HISTOCHEMICAL URATE OXIDASE ACTIVITY AND MICROBODIES IN NONHUMAN PRIMATE LIVER
Journal of Histochemistry and Cytochemistry - Tập 14 Số 9 - Trang 685-687 - 1966
Localization of alpha 2u-globulin within protein droplets of male rat kidney: immunohistochemistry using perfusion-fixed, GMA-embedded tissue sections. We investigated the light microscopic subcellular localization and quantitation of alpha 2u-globulin (alpha 2uG) in male rat kidney after 2,2,4-trimethylpentane exposure using a monoclonal antibody to alpha 2uG. Slices of perfusion-fixed kidney were cold-processed in glycolmethacrylate and the antigen localized after an avidin-biotin-horseradish peroxidase procedure with Hanker-Yates reag... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 37 Số 6 - Trang 813-818 - 1989
Alpha 2u-globulin in modified sebaceous glands with pheromonal functions: localization of the protein and its mRNA in preputial, meibomian, and perianal glands. alpha 2u-Globulin, the principal urinary protein of the male rat, has extensive sequence homology with many lipid binding proteins. The highest concentration of alpha 2u-globulin is found in the preputial gland, a holocrine secretory organ with pheromonal function. Meibomian and perianal glands are two other modified sebaceous glands with holocrine secretory cycles and pleiomorphic peroxi... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 37 Số 2 - Trang 149-157 - 1989
Chemical modification of horseradish peroxidase. Preparation and characterization of tracer enzymes with different isoelectric points. Horseradish peroxidase (HRP), a plant glycoprotein with a molecular weight of 40,000 D and a molecular radius (ae) of 30 A, has been modified chemically to prepare tracer molecules with different molecular charge. Modification of free carboxyl groups on the enzyme is achieved by carbodiimide activation and subsequent reaction of activated carboxyl groups with a nucleophile; uncharged grou... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 27 Số 10 - Trang 1352-1353 - 1979
MICRODETERMINATION OF CYTOCHROME OXIDASE IN RAT TISSUES BY THE OXIDATION OF N-PHENYL-p-PHENYLENEDIAMINE OR ASCORBIC ACID The cytochrome oxidase content of rat tissues was investigated, utilizing the accumulation of the free radical formed by the emzymatic, univalent oxidation of a stable, non-toxic substrate, N-phenyl- p-phenylenediamine ( p-aminodiphenylamine). This procedure eliminated the non-enzymatic coupling of the radical with α-naphthol, found in the classic Nadi reaction. Using homogenates, the fol... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 11 Số 1 - Trang 102-107 - 1963
Distribution of Corticosteroid Receptors in Mature Oligodendrocytes and Oligodendrocyte Progenitors of the Adult Mouse Brain The expression of glucocorticoid receptors (GRs) was investigated immunohistochemically in two different lineages of oligodendrocytes, using carbonic anhydrase (CA) II and neuron glial antigen (NG) 2 as markers of mature oligodendrocytes and oligodendrocyte progenitors, respectively. We focused on the gray matter regions, including CA1, CA3 and the dentate gyrus of the hippocampus, the pr... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 62 Số 3 - Trang 211-226 - 2014
A HISTOCHEMICAL PROCEDURE FOR THE LOCALIZATION OF CHOLINE ACETYLTRANSFERASE ACTIVITY A histochemical procedure has been developed for the demonstration of choline acetyltransferase activity. This procedure is based upon the formation of an insoluble lead mercaptide from coenzyme A (CoASH), a product of the enzymatic reaction. Optimal conditions for the precipitation of the lead mercaptide are defined. The localization of the reaction product in the rat spinal cord is rest... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 18 Số 6 - Trang 408-415 - 1970
Non-radioactive detection of nerve growth factor receptor (NGFR) mRNA in rat brain using in situ hybridization histochemistry. Radioactively labeled RNA probes in conjunction with in situ hybridization histochemistry have become a useful method for studying gene expression in the central nervous system. We used digoxigenin-labeled uridine triphosphate to synthesize cRNA probes for localization of nerve growth factor receptor (NGFR) mRNA in the rat basal forebrain. Detection of cells containing digoxigenin-labeled... ... hiện toàn bộ
Journal of Histochemistry and Cytochemistry - Tập 39 Số 2 - Trang 231-234 - 1991
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