Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth Factor

Journal of Histochemistry and Cytochemistry - Tập 45 Số 6 - Trang 795-804 - 1997
H. Francis Farhadi1, Sangeeta Pareek1, Robert Day2, Weijia Dong2, Michel Chrètien2, John Bergeron3, Nabil G. Seidah2, Richard A. Murphy1
1Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
2J.A. DeSeve Laboratories of Biochemical and Molecular Endocrinology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada
3Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada

Tóm tắt

Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from a 35-kD precursor by proteolytic enzymes that have yet to be identified. Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this study we questioned whether PCs could process salivary NGF in vivo. mRNA coding for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in situ hybridization within parasympathetic neurons of intralobular ganglia. Northern blot and in situ hybridization analyses also detect mRNA coding for furin. In SGs of male mice, furin mRNA levels are high at birth and remain high throughout development. In glands from female mice, levels decline during postnatal development and are lower in adults than in newborns. Immunocytochemistry detects furin immunoreactivity in pro-acinar and ductal cells of glands from newborn and pubescent mice. In glands of adults, furin immunoreactivity is detectable in acinar cells but highest levels are present in NGF-containing granular convoluted tubule cells. These data, taken together with those from previous studies, suggest that furin is a candidate processing enzyme for NGF in mouse submandibular glands.

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Thông tin xuất bản

Journal of Histochemistry and Cytochemistry

Tập 45 Số 6

795-804

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