Journal of General Physiology

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Using Total Fluorescence Increase (Signal Mass) to Determine the Ca2+ Current Underlying Localized Ca2+ Events
Journal of General Physiology - Tập 124 Số 3 - Trang 259-272 - 2004
Hui Zou, Lawrence M. Lifshitz, Richard A. Tuft, Kevin E. Fogarty, Joshua J. Singer
The feasibility of determining localized Ca2+ influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca2+ fluorescence transients (SCCaFTs), due to Ca2+ entry through single openings of Ca2+-permeable ion channels, while recording unitary channel currents. Since the image obtained with wide-field optics is an integration of both in-focus and ...... hiện toàn bộ
A Continuum Method for Determining Membrane Protein Insertion Energies and the Problem of Charged Residues
Journal of General Physiology - Tập 131 Số 6 - Trang 563-573 - 2008
Seungho Choe, Karen A. Hecht, Michael Grabe
Continuum electrostatic approaches have been extremely successful at describing the charged nature of soluble proteins and how they interact with binding partners. However, it is unclear whether continuum methods can be used to quantitatively understand the energetics of membrane protein insertion and stability. Recent translation experiments suggest that the energy required to insert char...... hiện toàn bộ
Sodium and Calcium Inward Currents in Freshly Dissociated Smooth Myocytes of Rat Uterus
Journal of General Physiology - Tập 110 Số 5 - Trang 565-577 - 1997
Masami Yoshino, S.Y. Wang, C. Y. Kao
Freshly dissociated myocytes from nonpregnant, pregnant, and postpartum rat uteri have been studied with the tight-seal patch-clamp method. The inward current contains both INa and ICa that are vastly different from those in tissue-cultured material. INa is abolished by Na+-free medium and by 1 μM tetrodotoxin. It first appears at ∼−40 mV, reaches maximum at 0 mV, and reverses at 84 mV. It...... hiện toàn bộ
On the Mechanism of Respiratory Control
Journal of General Physiology - Tập 49 Số 1 - Trang 149-162 - 1965
Ronald A. Butow, Efraim Racker
Control of oxidation is the key mechanism in the regulation of energy metabolism. In glycolysis the oxidation of glyceraldehyde-3-phosphate is controlled by DPNH, which inhibits glyceraldehyde-3-phosphate dehydrogenase. In oxidative phosphorylation the inhibition of electron flow from DPNH to oxygen, called "respiratory control," is the subject of this paper. After a discussion of the phys...... hiện toàn bộ
Phospholipase C in Living Cells
Journal of General Physiology - Tập 126 Số 3 - Trang 243-262 - 2005
Lisa F. Horowitz, Wiebke Hirdes, Byung‐Chang Suh, Donald W. Hilgemann, Ken Mackie, Bertil Hille
We have further tested the hypothesis that receptor-mediated modulation of KCNQ channels involves depletion of phosphatidylinositol 4,5-bisphosphate (PIP2) by phosphoinositide-specific phospholipase C (PLC). We used four parallel assays to characterize the agonist-induced PLC response of cells (tsA or CHO cells) expressing M1 muscarinic receptors: translocation of two fluorescent probes fo...... hiện toàn bộ
Gustatory responses of eel palatine receptors to amino acids and carboxylic acids.
Journal of General Physiology - Tập 74 Số 3 - Trang 301-317 - 1979
Kentaro Yoshii, Naoki Kamo, Kazuo Kurihara, Yonosuke Kobatake
The gustatory receptors of the eel palate were found to be extremely sensitive to amino acids and carboxylic acids. The results obtained are as follows: (a) 11 amino acids which are among naturally occurring amino acids elicited responses in the palatine nerve, but 9 amino acids did not elicit a response even at a high concentration. The effect of D-amino acids was always much less than th...... hiện toàn bộ
Proline Control of the Feeding Reaction of Cordylophora
Journal of General Physiology - Tập 46 Số 4 - Trang 823-837 - 1963
Chandler Fulton
The colonial hydroid Cordylophora is a carnivore whose feeding is induced by substances released from captured prey. An active molecule, probably the only one, has been isolated from a fraction of the laboratory food of Cordylophora, brine shrimp larvae, and identified on paper chromatograms as the imino acid proline. Reagent proline induces the feeding reaction at 10-5 M. The reaction is ...... hiện toàn bộ
Isoform-specific Stimulation of Cardiac Na/K Pumps by Nanomolar Concentrations of Glycosides
Journal of General Physiology - Tập 119 Số 4 - Trang 297-312 - 2002
Junyuan Gao, Randy S. Wymore, Yongli Wang, Glenn R. Gaudette, Irvin B. Krukenkamp, Ira S. Cohen, Richard T. Mathias
It is well-known that micromolar to millimolar concentrations of cardiac glycosides inhibit Na/K pump activity, however, some early reports suggested nanomolar concentrations of these glycosides stimulate activity. These early reports were based on indirect measurements in multicellular preparations, hence, there was some uncertainty whether ion accumulation/depletion rather than pump stim...... hiện toàn bộ
Modulation of voltage-dependent Ca channel current by arachidonic acid and other long-chain fatty acids in rabbit intestinal smooth muscle.
Journal of General Physiology - Tập 100 Số 1 - Trang 27-44 - 1992
Tadahito Shimada, A P Somlyo
The effects of arachidonic acid (AA) and other long-chain fatty acids on voltage-dependent Ca channel current (ICa) were investigated, with the whole cell patch clamp method, in longitudinal smooth muscle cells of rabbit ileum. 10-30 microM AA caused a gradual depression of ICa. The inhibitory effect of AA was not prevented by indomethacin (10 microM) (an inhibitor of cyclooxygenase) or no...... hiện toàn bộ
Mechanism of Arachidonic Acid Modulation of the T-type Ca2+ Channel α1G
Journal of General Physiology - Tập 124 Số 3 - Trang 225-238 - 2004
Karel Talavera, Mik Staes, Annelies Janssens, Guy Droogmans, Bernd Nilius
Arachidonic acid (AA) modulates T-type Ca2+ channels and is therefore a potential regulator of diverse cell functions, including neuronal and cardiac excitability. The underlying mechanism of modulation is unknown. Here we analyze the effects of AA on the T-type Ca2+ channel α1G heterologously expressed in HEK-293 cells. AA inhibited α1G currents within a few minutes, regardless of precedi...... hiện toàn bộ
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