Journal of Gene Medicine
SCOPUS (1998-2025)SCIE-ISI
1099-498X
1521-2254
Mỹ
Cơ quản chủ quản: WILEY , John Wiley and Sons Ltd
Lĩnh vực:
Genetics (clinical)Drug DiscoveryMolecular BiologyGeneticsMolecular Medicine
Phân tích ảnh hưởng
Các bài báo tiêu biểu
Gene therapy clinical trials worldwide to 2012 – an update
Tập 15 Số 2 - Trang 65-77 - 2013
AbstractTo date, over 1800 gene therapy clinical trials have been completed, are
ongoing or have been approved worldwide. Our database brings together global
information on gene therapy clinical trials from official agency sources,
published literature, conference presentations and posters kindly provided to us
by individual investigators or trial sponsors.This review presents our analysis
of clin...
Side‐effects of a systemic injection of linear polyethylenimine–DNA complexes
Tập 4 Số 1 - Trang 84-91 - 2002
AbstractBackgroundSystemic administration of linear polyethylenimine–DNA
complexes (L‐PEI/DNA) results in transient expression of the transgene in the
lung. This study analyzes the side‐effects associated with L‐PEI‐mediated
transfection.MethodsMice weighing from 16 to 25 g received increasing amounts of
L‐PEI/DNA intravenously. Gene expression was evaluated using luciferase as a
reporter gene. To...
A model for non‐viral gene delivery: through syndecan adhesion molecules and powered by actin
Tập 6 Số 7 - Trang 769-776 - 2004
AbstractBackgroundCell transfection requires cationic DNA complexes and heparan
sulfate proteoglycans (HSPGs) at the cell surface. Syndecans are transmembrane
HSPGs that are ubiquitously expressed on adherent cells. Their polyanionic
heparan sulfate moieties are bound at the distal end of their ectodomain, thus
facilitating interaction with large cationic particles.MethodsWe propose a model
for ce...
Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer
Tập 3 Số 2 - Trang 179-187 - 2001
Antibody targeted gene transfer to endothelium
Tập 5 Số 4 - Trang 311-323 - 2003
AbstractBackgroundOne of the drawbacks of the currently available vectors for
gene therapy is the lack of selectivity in gene delivery. We have therefore
investigated a strategy to generate immunoliposomes to target non‐viral vectors
to cell surface receptors on endothelium.Materials and methodsWe have developed
a novel method of coupling antibodies (Abs) to liposomes complexed to DNA, using
mild ...
Adenovirus efficiently transduces plasmacytoid dendritic cells resulting in TLR9‐dependent maturation and IFN‐α production
Tập 8 Số 11 - Trang 1300-1306 - 2006
AbstractBackgroundRecombinant replication‐deficient adenoviral vectors (recAd)
are attractive candidates for DNA vaccination approaches because they are able
to activate the innate and adaptive immune systems. Here we explore the ability
of recAd to transduce and activate subsets of dendritic cells, namely
plasmacytoid dendritic cells (pDC) and conventional dendritic cells
(cDC).MethodsDC were der...
Animal-free production of ccc-supercoiled plasmids for research and clinical applications
Tập 6 Số S1 - Trang S45-S53 - 2004
Intravenous glial‐derived neurotrophic factor gene therapy of experimental Parkinson's disease with Trojan horse liposomes and a tyrosine hydroxylase promoter
Tập 10 Số 3 - Trang 306-315 - 2008
AbstractBackgroundRats with experimental Parkinson's disease (PD) are treated
with intravenous glial‐derived neurotrophic factor (GDNF) plasmid DNA, non‐viral
gene therapy using Trojan horse liposomes (THLs) targeted with a monoclonal
antibody (MAb) to the rat transferrin receptor (TfR). Expression of the
transgene is confined to catecholaminergic cells by placement of the GDNF gene
under the infl...
Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography
Tập 11 Số 1 - Trang 79-88 - 2009
AbstractBackgroundIt is well known that the success of gene transfer to cells
and subsequent expression is strictly affected by the vector manufacturing
process. Several challenges encountered in the gene therapy field have
emphasized the need for the development of novel platforms that allow the
recovery of gene vectors and enable efficient transfection of cells. The use of
plasmid DNA‐based ther...