Journal of Gene Medicine

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Plasmid DNA purification
Journal of Gene Medicine - Tập 6 Số S1 - Trang S54-S66 - 2004
J. Stadler, Raf Lemmens, Tomas Nyhammar
Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography
Journal of Gene Medicine - Tập 11 Số 1 - Trang 79-88 - 2009
Fani Sousa, D.M.F. Prazeres, João A. Queiroz
AbstractBackgroundIt is well known that the success of gene transfer to cells and subsequent expression is strictly affected by the vector manufacturing process. Several challenges encountered in the gene therapy field have emphasized the need for the development of novel platforms that allow the recovery of gene vectors and enabl...... hiện toàn bộ
Comparison of toxicity and transfection efficiency of amphiphilic block copolymers and polycationic polymers in striated muscles
Journal of Gene Medicine - Tập 11 Số 3 - Trang 240-249 - 2009
Caroline Roques, Elias Fattal, Yves Fromes
AbstractBackgroundGene delivery using synthetic vectors is frequently based on cationic compounds such as polyethyleneimine (PEI). However, few data have been published on the ability of PEI to mediate transgene expression in muscle tissue. Besides cationic vectors, there is increasing interest focusing on amphiphilic copolymers a...... hiện toàn bộ
Association of albumin or protamine to lipoplexes: enhancement of transfection and resistance to serum
Journal of Gene Medicine - Tập 6 Số 6 - Trang 681-692 - 2004
Henrique Faneca, Sérgio Simões, Maria C. Pedroso de Lima
AbstractBackgroundThe successful application of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Such efficacy is strongly related to key parameters including serum resistance and protection of DNA.MethodsT...... hiện toàn bộ
Local tumour irradiation enhances the anti‐tumour effect of a double‐suicide gene therapy system in a murine glioma model
Journal of Gene Medicine - Tập 5 Số 5 - Trang 377-385 - 2003
Szilvia Désaknai, Katalin Lumniczky, Olga Ésik, Hirofumi Hamada, Géza Sáfrány
AbstractBackgroundGliomas are invasive malignant tumours with poor prognosis. Combination of gene directed enzyme pro‐drug therapy with existing treatment modalities might open new therapeutic potentials.MethodsMurine glioma 261 (Gl261) cells were transduced with an ad...... hiện toàn bộ
Triplex-forming molecules: from concepts to applications
Journal of Gene Medicine - Tập 3 Số 4 - Trang 299-310 - 2001
Marcella Faria, Carine Giovannangéli
Efficient targeted transduction of primary human endothelial cells with dual‐targeted lentiviral vectors
Journal of Gene Medicine - Tập 10 Số 3 - Trang 242-248 - 2008
Nonia Pariente, Si‐Hua Mao, Kouki Morizono, Irvin S. Y. Chen
AbstractAngiogenesis is a rate‐limiting factor for numerous human diseases. Angiogenic vessels and also the endothelium of certain organs such as the lung display molecular addresses that can be exploited for the selective delivery of gene therapeutics. Lentiviral vectors (LVs) are powerful tools for stable gene delivery but their integration and expression in unde...... hiện toàn bộ
Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer
Journal of Gene Medicine - Tập 3 Số 2 - Trang 179-187 - 2001
Virginie Escriou, Marie Carrière, Florence Bussone, Pierre Wils, Daniel Scherman
Effect of different parameters used for in vitro gene electrotransfer on gene expression efficiency, cell viability and visualization of plasmid DNA at the membrane level
Journal of Gene Medicine - Tập 15 Số 5 - Trang 169-181 - 2013
Saša Haberl, Maša Kandušer, Karel Flisar, Duša Hodžić, Vladimir B. Bregar, Damijan Miklavčič, Jean‐Michel Escoffre, Marie‐Pierre Rols, Mojca Pavlin
AbstractBackgroundGene electrotransfer is a nonviral method used for DNA delivery into cells. Several steps are involved. One of them is the interaction of DNA with the cell membrane, which is crucial before DNA can enter the cell. We analysed the level of DNA–membrane interaction in relation to electrotransfer efficiency and the ...... hiện toàn bộ
Characterisation of enzyme prodrug gene therapy combinations in coated spheroids and vascular networks in vitro
Journal of Gene Medicine - Tập 14 Số 1 - Trang 62-74 - 2012
Michelle Hunt, Dan Li, Michael P. Hay, Margaret J. Currie, Bridget Robinson, Adam V. Patterson, Gabi U. Dachs
AbstractBackgroundEnzyme prodrug gene therapy is designed as a targeted cancer treatment, destroying gene‐modified and bystander cells via exogenous enzyme‐generated cytotoxins. Targeting of tumour blood vessels using gene therapy is attractive, although optimal enzyme prodrug combinations have yet to be identified.... hiện toàn bộ
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