Journal of Dairy Research
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Proteinases in normal bovine milk and their action on caseins
Journal of Dairy Research - Tập 50 Số 1 - Trang 45-55 - 1983
SummaryNative proteolytic enzymes in good quality normal bovine milk readily
hydrolyscd the caseins during incubation or storage, producing the γ-caseins,
proteosc-peptone components 5 (PP5) and 8-fast (PP8F) and a considerable number
of other unidentified fragments, many of which were also subsequently found in
the proteose-pcptone fraction. The rate of casein hydrolysis was greater in
pasteurize...
Qualitative and quantitative determination of proteolysis in mastitic milks
Journal of Dairy Research - Tập 49 Số 4 - Trang 587-596 - 1982
SUMMARYProteolytic activity in mastitic skim-milk was often 5–10 fold higher
than in normal milk, its level being related to somatic cell count but not
precisely correlated with it. In milks with the highest levels of activity
plasmin accounted for about one third of the total proteinase. A further third
was sedimented with the micellar fraction together with the plasmin, but unlike
plasmin, was n...
Casein compositional studies: II. The effect of secretory disturbance on casein composition in freshly drawn and aged bovine milks
Journal of Dairy Research - Tập 48 Số 3 - Trang 437-446 - 1981
SummaryWhole casein preparations obtained from paired quarters (one healthy and
one subclinically infected) of each of 6 individual cows were fractionated
quantitatively by column chromatography on hydroxyapatite. Infection was
associated with an apparent decrease in the αs- and β-casein fractions and
increase in the γ- and κ-casein fractions. The increase in the κ-casein fraction
was attributable...
Influence of elevated somatic cell count on casein distribution and cheese-making
Journal of Dairy Research - Tập 47 Số 3 - Trang 393-400 - 1980
SummaryThe effects of increased somatic cell count, whether caused by infection
or by experimental infusion of bacterial endotoxin, on the distribution in milk
of caseins between the micellar and soluble forms were investigated. The
relationship of somatic cell count to some cheese-making parameters was also
studied. With quite modestly elevated cell counts (2–3 × 106/ml) increases of up
to 37% in...
38. The Inactivation of Lipase in Dairy Products by Traces of Heavy Metal Salts
Journal of Dairy Research - Tập 3 Số 3 - Trang 254-263 - 1932
Traces of heavy metals in amounts up to 25 parts per million have the property
of inhibiting the action of lipase in butter from unpasteurised sweet cream in
varying degrees depending on the concentration of the metal and on the metal
itself. Copper was the most potent metal of those studied, iron, nickel, cobalt,
manganese, and chromium being less active, while tin and aluminium had no
effect.The...
Chemiluminescence of bovine polymorphonuclear leucocytes during the periparturient period and relation with metabolic markers and bovine pregnancy-associated glycoprotein
Journal of Dairy Research - Tập 67 Số 2 - Trang 249-259 - 2000
The respiratory burst activity of polymorphonuclear leucocytes (PMN) was
evaluated in eight Holstein cows from 8 weeks before until 6 weeks after calving
by chemiluminescence (CL). The CL response started to decrease 1 week before
parturition, reaching a minimum during the first 2 weeks after calving. From
week 3 of lactation, CL increased again and returned to original levels by week
6 of lactati...
Evidence-based medicine: quality and comparability of clinical trials investigating the efficacy of prostaglandin F2α for the treatment of bovine endometritis
Journal of Dairy Research - Tập 79 Số 3 - Trang 287-296 - 2012
The objective of this study was to evaluate the quality and comparability of
published literature, and to summarize the effect of prostaglandin F2α (PGF2α)
for the treatment of endometritis. It has been postulated that there is a dearth
of high-level evidence-based research results in veterinary medicine. Also,
there is a marked variation in the quality of studies in veterinary and animal
science....
Kinetic studies ofin vivodigestion of bovine unheated skim-milk proteins in the rat stomach
Journal of Dairy Research - Tập 50 Số 1 - Trang 27-36 - 1983
SummaryThein vivoaction of gastric proteinases on bovine milk proteins was
studied in rats fed with skim-milk, by analysing gastric contents after 30, 60
and 240 min of digestion. Gastric proteolysis was already marked after 30 min of
digestion and liberated a large number of peptides with different molecular
weights. αs1-Casein and β-casein were still the main components of the stomach
contents t...
Process steps for the preparation of purified fractions of α-lactalbumin and β-lactoglobulin from whey protein concentrates
Journal of Dairy Research - Tập 66 Số 2 - Trang 225-236 - 1999
Fractions enriched with α-lactalbumin (α-la) and β-lactoglobulin (β-lg) were
produced by a process comprising the following successive steps:
clarification–defatting of whey protein concentrate, precipitation of
α-lactalbumin, separation of soluble β-lactoglobulin, washing the precipitate,
solubilization of the precipitate, concentration and purification of α-la. The
present study evaluated the pe...
Dietary trans octadecenoic acids upregulate the liver gene encoding Peroxisome Proliferator-Activated Receptor-α in transition dairy cows
Journal of Dairy Research - Tập 72 Số 1 - Trang 107-114 - 2005
Effects of feeding calcium salts of conjugated linoleic acid (CLA) or trans
octadecenoic acids (trans 18[ratio ]1) on lipid metabolism and hepatic contents
of mRNA encoding carnitine palmitoyltransferase 1 (CPT1), microsomal
triglyceride transfer protein (MTP) and peroxisome proliferator-activated
receptor alpha (PPARα) were examined in 15 early post-partum Holstein cows.
Dietary treatments were i...
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