wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella StrainsJournal of Clinical Microbiology - Tập 51 Số 12 - Trang 4073-4078 - 2013
Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis‐Chikhani, Carsten Struve, Dominique Decré
ABSTRACT
Pathogens of the genus
Klebsiella
have been classified into distinct capsular (K) types for nearly a century. K typing of
Klebsiella
species still ...... hiện toàn bộ
Prevalence of the Amylase-Binding Protein A Gene ( abpA ) in Oral StreptococciJournal of Clinical Microbiology - Tập 37 Số 12 - Trang 4081-4085 - 1999
Alan E. Brown, Jeffrey Rogers, Elaine M. Haase, Peter M. Zelasko, Frank A. Scannapieco
ABSTRACT
Salivary amylase binds specifically to a number of oral streptococcal species. This interaction may play an important role in dental plaque formation. Recently, a 585-bp gene was cloned and sequenced from
Streptococcus gordonii
Challis encoding a 20.5-kDa amylase-binding protein (Abp...... hiện toàn bộ
Improved Silica-Guanidiniumthiocyanate DNA Isolation Procedure Based on Selective Binding of Bovine Alpha-Casein to Silica ParticlesJournal of Clinical Microbiology - Tập 37 Số 3 - Trang 615-619 - 1999
René Boom, C. J. A. Sol, Marcel Beld, Jan Weel, Jaap Goudsmit, Pauline Wertheim‐van Dillen
ABSTRACT
DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition could be relieved by the use of a novel lysis buffer containing alpha-casein. When the novel ...... hiện toàn bộ
Use of an ATP bioluminescent assay to evaluate viability of Pneumocystis carinii from ratsJournal of Clinical Microbiology - Tập 32 Số 11 - Trang 2791-2800 - 1994
F Chen, Melanie T. Cushion
A bioluminescent assay which employs the luciferin-luciferase ATP-dependent reaction was used to evaluate the viability of populations of Pneumocystis carinii derived from infected rat lungs. Contamination with host cells was reduced by a purification method which involved a combination of low- and high-speed centrifugations resulting in a 1,000-fold reduction of the rat cells while enrich...... hiện toàn bộ