Journal of Clinical Microbiology

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Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses
Journal of Clinical Microbiology - Tập 38 Số 2 - Trang 578-585 - 2000
Chiho Matsumoto, Jun Okuda, Masahisa Ishibashi, Masaaki Iwanaga, Pallavi Garg, Thandavarayan Rammamurthy, Hin‐chung Wong, Angelo DePaola, Yung Bu Kim, M. John Albert, Mitsuaki Nishibuchi
ABSTRACT Vibrio parahaemolyticus O3:K6 strains responsible for the increase in the number of cases of diarrhea in Calcutta, India, beginning in February 1996 and those isolated from Southeast Asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the ...... hiện toàn bộ
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis of smooth-lipopolysaccharide heterogeneity among Brucella biovars related to A and M specificities
Journal of Clinical Microbiology - Tập 28 Số 10 - Trang 2169-2174 - 1990
Bruno Garin‐Bastuji, Robert A. Bowden, G Dubray, J. N. Limet
Smooth (S)-lipopolysaccharide (LPS) preparations from reference and field strains of several biovars of Brucella abortus, B. melitensis, and B. suis were prepared by (i) the hot phenol-water method, (ii) hot sodium dodecyl sulfate extraction and proteinase K digestion, or (iii) dimethyl sulfoxide extraction. These S-LPS-enriched fractions were further analyzed by sodium dodecyl sulfate-pol...... hiện toàn bộ
wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
Journal of Clinical Microbiology - Tập 51 Số 12 - Trang 4073-4078 - 2013
Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis‐Chikhani, Carsten Struve, Dominique Decré
ABSTRACT Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still ...... hiện toàn bộ
Prevalence of the Amylase-Binding Protein A Gene ( abpA ) in Oral Streptococci
Journal of Clinical Microbiology - Tập 37 Số 12 - Trang 4081-4085 - 1999
Alan E. Brown, Jeffrey Rogers, Elaine M. Haase, Peter M. Zelasko, Frank A. Scannapieco
ABSTRACT Salivary amylase binds specifically to a number of oral streptococcal species. This interaction may play an important role in dental plaque formation. Recently, a 585-bp gene was cloned and sequenced from Streptococcus gordonii Challis encoding a 20.5-kDa amylase-binding protein (Abp...... hiện toàn bộ
Utility of Major Leukocyte Subpopulations for Monitoring Secondary Cytomegalovirus Infections in Renal-Allograft Recipients by PCR
Journal of Clinical Microbiology - Tập 36 Số 4 - Trang 1008-1014 - 1998
Peter Schäfer, W. Tenschert, Liana Cremaschi, K. Gutensohn, Rainer Laufs
ABSTRACT The feasibility of the major peripheral blood leukocyte (PBL) subsets for use in qualitative and quantitative PCR to monitor secondary cytomegalovirus (CMV) infection and ganciclovir therapy was assessed with 188 blood samples derived from 40 CMV immunoglobulin G-positive renal-allograft recipients. In pp65 antigen-positive patients all leukocyte...... hiện toàn bộ
Rising Levels of Human Cytomegalovirus (HCMV) Antigenemia during Initial Antiviral Treatment of Solid-Organ Transplant Recipients with Primary HCMV Infection
Journal of Clinical Microbiology - Tập 36 Số 4 - Trang 1113-1116 - 1998
Giuseppe Gerna, Maurizio Zavattoni, Elena Percivalle, Paolo Grossi, Maria Torsellini, Maria Grazia Revello
ABSTRACT In 7 of 18 solid-organ transplant recipients with primary human cytomegalovirus (HCMV) infection, HCMV antigenemia levels were unexpectedly found to rise significantly ( P = 0.018) during a mean time of 7.3 ± 3.2 days after initiation of specific antiviral treatment, whereas correspo...... hiện toàn bộ
Standardization of the Human Cytomegalovirus Antigenemia Assay by Means of In Vitro-Generated pp65-Positive Peripheral Blood Polymorphonuclear Leukocytes
Journal of Clinical Microbiology - Tập 36 Số 12 - Trang 3585-3589 - 1998
Giuseppe Gerna, Elena Percivalle, Maria Torsellini, Maria Grazia Revello
ABSTRACT We generated in vitro human cytomegalovirus (HCMV) pp65-positive polymorphonuclear leukocytes (PMN) resembling those detected in vivo, following cocultivation of PMN from healthy donors and wild-type HCMV-infected endothelial cells or fibroblasts. After purification, PMN are suitable for preparation of cytospots which can be used for the antigene...... hiện toàn bộ
Curtobacterium flaccumfaciens Septic Arthritis following Puncture with a Coxspur Hawthorn Thorn
Journal of Clinical Microbiology - Tập 49 Số 7 - Trang 2759-2760 - 2011
Michelle Francis, Richard Doherty, Minoo Patel, John Hamblin, Samar Ojaimi, Tony M. Korman
ABSTRACT Curtobacterium species are recognized plant pathogens. We report the first well-documented case of Curtobacterium human infection, a child with septic arthriti...... hiện toàn bộ
Improved Silica-Guanidiniumthiocyanate DNA Isolation Procedure Based on Selective Binding of Bovine Alpha-Casein to Silica Particles
Journal of Clinical Microbiology - Tập 37 Số 3 - Trang 615-619 - 1999
René Boom, C. J. A. Sol, Marcel Beld, Jan Weel, Jaap Goudsmit, Pauline Wertheim‐van Dillen
ABSTRACT DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition could be relieved by the use of a novel lysis buffer containing alpha-casein. When the novel ...... hiện toàn bộ
Use of an ATP bioluminescent assay to evaluate viability of Pneumocystis carinii from rats
Journal of Clinical Microbiology - Tập 32 Số 11 - Trang 2791-2800 - 1994
F Chen, Melanie T. Cushion
A bioluminescent assay which employs the luciferin-luciferase ATP-dependent reaction was used to evaluate the viability of populations of Pneumocystis carinii derived from infected rat lungs. Contamination with host cells was reduced by a purification method which involved a combination of low- and high-speed centrifugations resulting in a 1,000-fold reduction of the rat cells while enrich...... hiện toàn bộ
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