Journal of Clinical Microbiology

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Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses
Journal of Clinical Microbiology - Tập 38 Số 2 - Trang 578-585 - 2000
Chiho Matsumoto, Jun Okuda, Masahisa Ishibashi, Masaaki Iwanaga, Pallavi Garg, Thandavarayan Rammamurthy, Hin‐chung Wong, Angelo DePaola, Yung Bu Kim, M. John Albert, Mitsuaki Nishibuchi
ABSTRACT Vibrio parahaemolyticus O3:K6 strains responsible for the increase in the number of cases of diarrhea in Calcutta, India, beginning in February 1996 and those isolated from Southeast Asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the ...... hiện toàn bộ
First Clinical Report of Pandemic Vibrio parahaemolyticus O3:K6 Infection in Italy
Journal of Clinical Microbiology - Tập 46 Số 6 - Trang 2144-2145 - 2008
Donatella Ottaviani, Francesca Leoni, Elena Rocchegiani, Sabrina Santarelli, Cristina Canonico, Laura Masini, Vittoria DiTrani, Antonio Carraturo
Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction
Journal of Clinical Microbiology - Tập 27 Số 12 - Trang 2751-2757 - 1989
Helge Karch, Thomas F. Meyer
By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectable in any of 20 nontoxigenic E. coli strains. The primers deduced from a ...... hiện toàn bộ
Approach to Diagnosis of Cardiovascular Implantable-Electronic-Device Infection
Journal of Clinical Microbiology - Tập 56 Số 7 - 2018
Daniel C. DeSimone, M. Rizwan Sohail
ABSTRACT Device infection remains a significant challenge as clinical indications for cardiovascular implantable electronic device (CIED) therapy continue to expand beyond the prevention and treatment of cardiac arrhythmias. Patients receiving CIED therapy are now older and have significant comorbidities, placing them at higher risk of complications, incl...... hiện toàn bộ
Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA
Journal of Clinical Microbiology - Tập 29 Số 3 - Trang 573-577 - 1991
Mark Schiffman, Heidi M. Bauer, Attila T. Lőrincz, M. Michele Manos, Janet C. Byrne, Andrew G. Glass, D. M. Cadell, Peter M. Howley
A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by cervicovaginal lavage. The study population included 32 women with condylo...... hiện toàn bộ
Comparison of dot filter hybridization, Southern transfer hybridization, and polymerase chain reaction amplification for diagnosis of anal human papillomavirus infection
Journal of Clinical Microbiology - Tập 31 Số 4 - Trang 1003-1006 - 1993
Jane Kuypers, Cathy W. Critchlow, Patti E. Gravitt, D Vernon, James Sayer, M. Michele Manos, Nancy B. Kiviat
The detection and classification of human papillomavirus (HPV) by a consensus primer polymerase chain reaction (PCR) technique were compared with detection and classification by dot filter hybridization (DFH) and Southern transfer hybridization (STH). PCR detected HPV in 87% of specimens; the detection rates for DFH and STH were 51% and 49%, respectively. The specific HPV types detected by...... hiện toàn bộ
Nylon bead enzyme-linked immunosorbent assay for detection of sub-picogram quantities of Brucella antigens
Journal of Clinical Microbiology - Tập 18 Số 3 - Trang 601-608 - 1983
Vindya Perera, Min T. Creasy, A J Winter
An indirect sandwich enzyme-linked immunosorbent assay, using antibody covalently coupled to nylon beads, has been adapted for the detection of Brucella antigens. Optimum conditions were achieved by incubation of 1 ml of reaction mixture with a single bead, and by minimizing nonspecific interactions through the use of beads coated with purified bovine antibodies, preabsorption of third lay...... hiện toàn bộ
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis of smooth-lipopolysaccharide heterogeneity among Brucella biovars related to A and M specificities
Journal of Clinical Microbiology - Tập 28 Số 10 - Trang 2169-2174 - 1990
Bruno Garin‐Bastuji, Robert A. Bowden, G Dubray, J. N. Limet
Smooth (S)-lipopolysaccharide (LPS) preparations from reference and field strains of several biovars of Brucella abortus, B. melitensis, and B. suis were prepared by (i) the hot phenol-water method, (ii) hot sodium dodecyl sulfate extraction and proteinase K digestion, or (iii) dimethyl sulfoxide extraction. These S-LPS-enriched fractions were further analyzed by sodium dodecyl sulfate-pol...... hiện toàn bộ
Preparation by ultrafiltration and control by high-performance liquid chromatography of the native hapten of Brucella abortus for use in radial immunodiffusion diagnostic test
Journal of Clinical Microbiology - Tập 25 Số 10 - Trang 1860-1863 - 1987
Michel S. Zygmunt, G Dubray
An effective method was developed for the preparation of Brucella abortus native hapten by use of an ultrafiltration system. The membranes PM30 and YM5 were used to separate the lipopolysaccharide and the native hapten. The yield of the native hapten was higher than that obtained by a more complex procedure reported previously. This method is economical for the large-scale preparation of B...... hiện toàn bộ
wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
Journal of Clinical Microbiology - Tập 51 Số 12 - Trang 4073-4078 - 2013
Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis‐Chikhani, Carsten Struve, Dominique Decré
ABSTRACT Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still ...... hiện toàn bộ
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