wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains

Journal of Clinical Microbiology - Tập 51 Số 12 - Trang 4073-4078 - 2013
Sylvain Brisse1,2, Virginie Passet1,2, Anita Björk Haugaard3,4, Anamaria Babosan5, Najiby Kassis‐Chikhani6, Carsten Struve3,4, Dominique Decré5
1CNRS, UMR3525, Paris, France
2Institut Pasteur, Microbial Evolutionary Genomics, Paris, France
3Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark
4WHO Collaborating Centre for Reference and Research on Escherichia and Klebsiella, Statens Serum Institut, Copenhagen, Denmark
5Département de Bactériologie, Université Pierre et Marie Curie, Faculté de Médecine, and Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Est Parisiens, Paris, France
6Laboratoire de Bactériologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France

Tóm tắt

ABSTRACT Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still has important applications in epidemiology and clinical microbiology, but the serological method has strong practical limitations. Our objective was to evaluate the sequencing of wzi , a gene conserved in all capsular types of Klebsiella pneumoniae that codes for an outer membrane protein involved in capsule attachment to the cell surface, as a simple and rapid method for the prediction of K type. The sequencing of a 447-nucleotide region of wzi distinguished the K-type reference strains with only nine exceptions. A reference wzi sequence database was created by the inclusion of multiple strains representing K types associated with high virulence and multidrug resistance. A collection of 119 prospective clinical isolates of K. pneumoniae were then analyzed in parallel by wzi sequencing and classical K typing. Whereas K typing achieved typeability for 81% and discrimination for 94.4% of the isolates, these figures were 98.1% and 98.3%, respectively, for wzi sequencing. The prediction of K type once the wzi allele was known was 94%. wzi sequencing is a rapid and simple method for the determination of the K types of most K. pneumoniae clinical isolates.

Từ khóa


Tài liệu tham khảo

10.1128/JCM.00676-11

10.4161/viru.22718

10.1086/516369

10.1111/j.1574-6976.2011.00268.x

10.1128/JCM.43.8.4178-4182.2005

10.1046/j.1469-0691.2002.00411.x

10.1099/jmm.0.2008/003798-0

10.1099/jmm.0.46964-0

10.1093/jac/dkn287

10.1016/j.diagmicrobio.2013.01.006

10.1084/jem.44.5.683

ØrskovIØrskovF. 1984. Serotyping of Klebsiella. Methods Microbiol. 14:117–133.

10.1128/iai.40.1.56-61.1983

10.1086/519262

10.1136/gut.50.3.420

10.1128/JCM.42.8.3388-3398.2004

10.1128/JCM.01716-07

10.1099/jmm.0.46165-0

10.1086/512686

10.1128/JCM.01150-06

10.3201/eid1307.070187

10.1128/jcm.23.4.687-690.1986

10.1128/JCM.37.9.2808-2812.1999

10.1371/journal.pone.0004982

10.1128/jb.177.7.1788-1796.1995

10.1371/journal.pntd.0001052

10.1016/j.str.2013.03.010

10.1128/JB.185.19.5882-5890.2003

10.1099/00207713-51-3-915

10.1017/S0022172400025043

OrskovFOrskovI. 1972. Immunoelectrophoretic patterns of extracts from Escherichia coli O antigen test strains O1 to O157 examinations in homologous OK sera. Further comments on the classification of Escherichia K antigens. Acta Pathol. Microbiol. Scand. B Microbiol. Immunol. 80:905–910.

10.1128/JB.00796-07

10.1093/molbev/msr121

10.1099/00207713-51-3-925

GrimontPADGrimontF. 2005. Genus Klebsiella, p 685–693. In BrennerDJKriegNRStaleyJT (ed), Bergey's manual of systematic bacteriology, vol 2: the proteobacteria, part B: the gammaproteobacteria. Springer-Verlag, New York, NY.

10.1078/0723-2020-00261

10.1007/s00284-012-0245-x

10.1128/JCM.01916-08

10.1371/journal.pone.0003018

10.1128/AAC.49.12.5149-5152.2005

10.1128/JB.00315-09

10.1099/mic.0.029017-0

10.1128/JB.181.7.2307-2313.1999

10.1128/JCM.00624-11

10.1111/j.1469-0691.2004.00973.x

10.1128/CMR.11.4.589

10.1186/1471-2105-11-595

Thông tin
Thông tin xuất bản

Journal of Clinical Microbiology

Tập 51 Số 12

4073-4078

Thông tin tác giả