Selective staining of intracellular inclusions of tobacco mosaic virusInstitute of Experimental Botany - Tập 7 - Trang 374-375 - 1965
Véra Sosnová
For specific staining of TMV inclusions the cytochemical method of Hršel for the determination of tryptophan containing proteins was used. This method makes it possible to obtain series of sections in contrast to the methods employed up to the present, so that TMV inclusions from any part of the plant can be detected. Staining was tested “in situ” as well as in preparations of isolated virus parti...... hiện toàn bộ
Effect of Soil Moisture on the Gas Exchange of Changium smyrnioides and Anthriscus sylvestrisInstitute of Experimental Botany - Tập 47 - Trang 605-608 - 2003
Y. Ge, J. Chang, W.-C. Li, H.-Y. Sheng, C.-L. Yue, G.Y.S. Chan
The effect of soil moisture on gas exchange and growth of an endangered species, Changium smyrnioides, was compared with a non-endangered species, Anthriscus sylvestris. The two species belong to the same family Umbellaceae. With the decrease of soil moisture, the net photosynthetic rate (PN) and transpiration rate (E) decreased, while water use efficiency (WUE) increased, PN and WUE of C. smyrnio...... hiện toàn bộ
Cloning, identification, and expression analysis of a Dicer-Like gene family from Solanum lycopersicumInstitute of Experimental Botany - Tập 60 - Trang 410-418 - 2016
T. Wang, L. You, R. Li, D.-Q. Fu, B.-Z. Zhu, Y.-B. Luo, H.-L. Zhu
Dicer proteins belong to the RNase III family of proteins, which are key components in small RNA biogenesis. In Solanum lycopersicum, seven Dicer-like (DCL) genes have been identified and have been named SlDCL. In this study, we cloned the full-length sequence of the SlDCL genes including untranslated regions using RNA ligase-mediated rapid amplification of cDNA ends. Our analysis indicates that 7...... hiện toàn bộ
Subcellular localization and polymorphism of peroxidase in horse-radish tumour and teratoma tissueInstitute of Experimental Botany - Tập 39 - Trang 575-582 - 1997
T. Peškan, M.A. Pedreño, M. Krsnik-Rasol, R. Muñoz
The localization of peroxidase in cells of horse-radish (Armoracia lapathifolia Gilib.) tumour and teratoma tissues was studied. Both tissue lines were derived from the same primary crown-gall tumour induced on the leaf fragments by a wild type of Agrobacterium tumefaciens B6S3. Enzymatic activity was measured in cell walls, high-density heterogeneous membrane fraction, microsomal and soluble (no ...... hiện toàn bộ
Age-associated alterations in DNA methylation and expression of methyltransferase and demethylase genes in Arabidopsis thalianaInstitute of Experimental Botany - Tập 60 - Trang 628-634 - 2016
Z. V. Ogneva, A. S. Dubrovina, K. V. Kiselev
Little is known about the contributions of DNA methylation/demethylation to plant aging and senescence. We used Arabidopsis thaliana to study how increasing age of an annual plant species influences DNA methylation. Based on methylation-sensitive DNA fragmentation assay, it could be concluded that aging A. thaliana was accompanied by DNA demethylation. Bisulfite sequencing reveals that cytosine me...... hiện toàn bộ
Retarding effect of inhibitors of protein and RNA synthesis on chlorophyll and protein breakdownInstitute of Experimental Botany - Tập 14 Số 2 - Trang 146-154 - 1972
Knypl, J. S., Mazurczyk, W.
Cycloheximide, ethionine,p-fluorophenylalanine, 6-azauracil, 5-diazouracil and vanillin, applied at relatively high concentrations, retarded the yellowing of kale (Brassica oleracea L. var.acephala) leaf discs in darkness, and stimulated it in light. All the compounds inhibited protein synthesis and retarded protein breakdown. Cycloheximide,p-fluorophenylalanine, diazouracil and vanillin also inhi...... hiện toàn bộ
Evaluation of zinc accumulation potential of Hydrilla verticillataInstitute of Experimental Botany - Tập 53 - Trang 789-792 - 2009
S. Srivastava, S. Mishra, S. Dwivedi, R. D. Tripathi, P. K. Tandon, D. K. Gupta
Biofortification of foods with essential micronutrients and phytoremediation of the contaminated sites are the two sides of the same coin for metals like zinc. In the present study, Zn accumulation potential, growth and antioxidant status of Hydrilla verticillata (L.f.) Royle plants were studied upon supplementation of Zn (0–5 000 µM) for 2 and 7 d. At 5000 µM Zn, plants accumulated about 7.60 and...... hiện toàn bộ