Selective staining of intracellular inclusions of tobacco mosaic virus
Tóm tắt
For specific staining of TMV inclusions the cytochemical method of Hršel for the determination of tryptophan containing proteins was used. This method makes it possible to obtain series of sections in contrast to the methods employed up to the present, so that TMV inclusions from any part of the plant can be detected. Staining was tested “in situ” as well as in preparations of isolated virus particles.
Tài liệu tham khảo
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Hršel, I.: Färbung von Tryptophan enhaltenden Eiweissstoffen nach der CrO3-Eosin-Lichtgrün-Methode. —Acta Histochemica4: 47–64, 1957.
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