New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesisGenes and Development - Tập 21 Số 5 - Trang 601-614 - 2007
Michael G. Smith, Tara A. Gianoulis, Stefan Pukatzki, John J. Mekalanos, L. Nicholas Ornston, Mark Gerstein, M Snyder
Acinetobacter baumannii has emerged as an important and problematic human pathogen as it is the causative agent of several types of infections including pneumonia, meningitis, septicemia, and urinary tract infections. We explored the pathogenic content of this harmful pathogen using a combination of DNA sequencing and insertional mutagenesis. The genome of this o...... hiện toàn bộ
Functional overlap between Sgs1–Top3 and the Mms4–Mus81 endonucleaseGenes and Development - Tập 15 Số 20 - Trang 2730-2740 - 2001
Vivek Kaliraman, Janet R. Mullen, William M. Fricke, Suzanne A. Bastin-Shanower, Steven J. Brill
The RecQ DNA helicases, human BLM and yeast Sgs1, form a complex with topoisomerase III (Top3) and are thought to act during DNA replication to restart forks that have paused due to DNA damage or topological stress. We have shown previously that yeast cells lackingSGS1 or TOP3 require MMS4 and MUS...... hiện toàn bộ
The PTEN-regulating microRNA miR-26a is amplified in high-grade glioma and facilitates gliomagenesis in vivoGenes and Development - Tập 23 Số 11 - Trang 1327-1337 - 2009
Jason T. Huse, Cameron Brennan, Dolores Hambardzumyan, Boyoung Wee, John Pena, Sara H. Rouhanifard, Cherin Sohn-Lee, Carlos le Sage, Reuven Agami, Thomas Tuschl, Eric C. Holland
Activated oncogenic signaling is central to the development of nearly all forms of cancer, including the most common class of primary brain tumor, glioma. Research over the last two decades has revealed the particular importance of the Akt pathway, and its molecular antagonist PTEN (phosphatase and tensin homolog), in the process of gliomagenesis. Recent studies have also demonstrated that...... hiện toàn bộ
Maternal PPARγ protects nursing neonates by suppressing the production of inflammatory milkGenes and Development - Tập 21 Số 15 - Trang 1895-1908 - 2007
Yihong Wan, Alan Saghatelian, Ling-Wa Chong, Chun‐Li Zhang, Benjamin F. Cravatt, Ronald M. Evans
Lactation is a highly demanding lipid synthesis and transport process that is crucial for the development of newborn mammals. While PPARγ is known to promote adipogenesis and lipogenesis in adipose tissue, its role in the lactating mammary gland is unexplored. Here, we report that a targeted deletion of PPARγ in mice results in the production of “toxic milk” containing elevated levels of i...... hiện toàn bộ
Splicing-independent loading of TREX on nascent RNA is required for efficient expression of dual-strand piRNA clusters in DrosophilaGenes and Development - Tập 30 Số 7 - Trang 840-855 - 2016
Junho K. Hur, Yicheng Luo, Sungjin Moon, Maria Ninova, Georgi K. Marinov, Yun Doo Chung, Alexei A. Aravin
The conserved THO/TREX (transcription/export) complex is critical for pre-mRNA processing and mRNA nuclear export. In metazoa, TREX is loaded on nascent RNA transcribed by RNA polymerase II in a splicing-dependent fashion; however, how TREX functions is poorly understood. Here we show that Thoc5 and other TREX components are essential for the biogenesis of piRNA, a distinct class of small ...... hiện toàn bộ
Encoding NF-κB temporal control in response to TNF: distinct roles for the negative regulators IκBα and A20Genes and Development - Tập 22 Số 15 - Trang 2093-2101 - 2008
Shannon L. Werner, Jeffrey D. Kearns, Victoria Zadorozhnaya, Candace Lynch, Ellen L O'Dea, Mark P. Boldin, Averil Ma, David Baltimore, Alexander Hoffmann
TNF-induced NF-κB activity shows complex temporal regulation whose different phases lead to distinct gene expression programs. Combining experimental studies and mathematical modeling, we identify two temporal amplification steps—one determined by the obligate negative feedback regulator IκBα—that define the duration of the first phase of NF-κB activity. The second phase is defined by A20,...... hiện toàn bộ
Cytosine methylation and mammalian developmentGenes and Development - Tập 13 Số 1 - Trang 26-34 - 1999
Colum P. Walsh, Timothy H. Bestor
Programmed methylation and demethylation of regulatory sequences has been proposed to play a central role in vertebrate development. We report here that the methylation status of the 5′ regions of a panel of tissue-specific genes could not be correlated with expression in tissues of fetal and newborn mice. Genes reported to be regulated by reversible methylation were not expressed ectopica...... hiện toàn bộ
A role for microRNAs in maintenance of mouse mammary epithelial progenitor cellsGenes and Development - Tập 21 Số 24 - Trang 3238-3243 - 2007
Ingrid Ibarra, Yaniv Erlich, Senthil K. Muthuswamy, Ravi Sachidanandam, Gregory J. Hannon
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dβ, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cel...... hiện toàn bộ