Gene Analysis Techniques
0735-0651
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Các bài báo tiêu biểu
High-level bacterial expression and purification of human T-lymphotropic virus type I (HTLV-I) transmembrane env protein
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A rapid and sensitive assay procedure for chloramphenicol acetyl transferase application to eukaryotic gene promoter function
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Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer
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Affinity purification of specific DNA fragments using a lac repressor fusion protein
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Analysis of recombinant plasmids using multiple agarose slab gels in series
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Hybridization of Oligo(dT) to RNA on nitrocellulose
Tập 4 - Trang 17 - 1987
Quantitation of mRNA immobilized on nitrocellulose filters is an essential aspect of some studies in molecular biology. Hybridization of oligo(dT)18 to the poly(A) tails of mRNA can be used to measure filter-bound mRNA and thus provides a basis for comparing abundance of specific mRNAs. Hybridization rate of 32P-labeled oligo(dT)18 in 0.75 M NaCl, 75 mM sodium citrate, pH 7 (5 × SSC) to immobilize......