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Gene Analysis Techniques

  0735-0651

 

 

 

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Direct sequencing of PCR products using the Maxam-Gilbert method
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Hybridization of Oligo(dT) to RNA on nitrocellulose
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Harley Calvin B.
Quantitation of mRNA immobilized on nitrocellulose filters is an essential aspect of some studies in molecular biology. Hybridization of oligo(dT)18 to the poly(A) tails of mRNA can be used to measure filter-bound mRNA and thus provides a basis for comparing abundance of specific mRNAs. Hybridization rate of 32P-labeled oligo(dT)18 in 0.75 M NaCl, 75 mM sodium citrate, pH 7 (5 × SSC) to immobilize...... hiện toàn bộ
Storage of spheroplasts at −70°C for transfection with φX174 RFDNA
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A synthetic translation-terminator gene
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Fragment tagging
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Jill H Zeilstra-Ryalls, Ronald L Somerville
Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer
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Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer
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