Hybridization of Oligo(dT) to RNA on nitrocelluloseGene Analysis Techniques - Tập 4 - Trang 17 - 1987
Harley Calvin B.
Quantitation of mRNA immobilized on nitrocellulose filters is an essential aspect of some studies in molecular biology. Hybridization of oligo(dT)18 to the poly(A) tails of mRNA can be used to measure filter-bound mRNA and thus provides a basis for comparing abundance of specific mRNAs. Hybridization rate of 32P-labeled oligo(dT)18 in 0.75 M NaCl, 75 mM sodium citrate, pH 7 (5 × SSC) to immobilize...... hiện toàn bộ