Current Microbiology
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Diversity of Lactic Acid Bacteria Associated with Fresh Coffee Cherries in Taiwan
Current Microbiology - Tập 68 - Trang 440-447 - 2013
A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and
Weissella species were the most common LAB found in two farms located at an approximate altitude of 800 m. ......
Bluetongue virus: Detection of antiviral immunoglobulin G by means of enzyme-linked immunosorbent assay
Current Microbiology - Tập 4 - Trang 381-385 - 1980
An enzyme-linked immunosorbent assay was developed to detect antiviral IgG in the sere of sheep exposed to bluetongue virus. It was found that the enzyme-linked immunosorbent assay is a rapid and sensitive method for the detection of anti-bluetongue virus antibody. Bluetongue virus antigen prepared from extracts of virus infected BHK and Vero cells were equally effective. Antigen prepared from uni......
Flow-Cytometric Method for Viability Analysis of Mycoplasma gallisepticum and Other Cell-Culture-Contaminant Mollicutes
Current Microbiology - Tập 78 - Trang 67-77 - 2020
Mycoplasma is the smallest self-replicating bacteria, figuring as common contaminant of eukaryotic cell cultures. Production inputs and operator’s manipulation seem to be the main sources of such contamination. Many analytical approaches have been applied for mycoplasma detection in cell cultures and also in biological products. However, unless they were validated, only indicator cell culture and ......
Localization and Characterization of GTP-Binding Protein CT703 in the Chlamydia trachomatis-Infected Cells
Current Microbiology - Tập 62 - Trang 465-471 - 2010
To localize and characterize the GTP-binding protein encoded by the chlamydial ORF CT703 in the Chlamydia trachomatis-infected cells, the gene coding for CT703 in the Chlamydia
trachomatis serovar L2 genome was cloned into the prokaryotic expression vector pGEX and expressed as GST fusion protein in the E. coli BL21 strain. The GST-CT703 fusion protein was purified and used......
Optically Trapped Bacteria Pairs Reveal Discrete Motile Response to Control Aggregation upon Cell–Cell Approach
Current Microbiology - Tập 69 - Trang 669-674 - 2014
Aggregation of bacteria plays a key role in the formation of many biofilms. The critical first step is cell–cell approach, and yet the ability of bacteria to control the likelihood of aggregation during this primary phase is unknown. Here, we use optical tweezers to measure the force between isolated Bacillus subtilis cells during approach. As we move the bacteria towards each other, cell motility......
Pulsed-Field Gel Electrophoresis Analysis of the Genome of Rhodococcus fascians: Genome Size and Linear and Circular Replicon Composition in Virulent and Avirulent Strains
Current Microbiology - Tập 36 - Trang 302-308 - 1998
Total DNA of virulent and avirulent strains of Rhodococcus fascians was resolved by pulsed-field gel electrophoresis (PFGE) into a discrete number of fragments by digestion with the endonucleases AseI and DraI. Restriction endonucleases PacI, PmeI, and SwaI yielded no fragments upon digestion of R. fascians genome, and all the other tested endonucleases recognizing 6 bp released too many fragment......
A Genomically Modified Marker Strain of Escherichia coli
Current Microbiology - Tập 37 - Trang 341-346 - 1998
Contamination of the environment with human sewage represents a serious public health concern in which Escherichia coli plays a central role, either directly as a human pathogen or indirectly through its use as an indicator organism. There is thus an ongoing effort to better understand the behavior of E. coli within such environments. Useful to such studies is the ability to readily detect a spec......
Molecular Characterization of Chitinase Genes from a Local Isolate of Serratia marcescens and Their Contribution to the Insecticidal Activity of Bacillus thuringiensis Strains
Current Microbiology - Tập 67 - Trang 499-504 - 2013
The chitinase B (chiB) and C (chiC) genes and flanking regions from a local isolate of Serratia marcescens were cloned individually and sequenced. Results showed that these chiB and chiC genes have a 96 % maximum similarity with chiB and chiC from different S. marcescens species (GenBank numbers Z36295.1 and AJ630582.1, respectively). The amplified chiB fragment, including some upstream and downst......
Transcriptional Regulation of β-Glucanase Activity in the Ruminal Bacterium, Prevotella bryantii B14
Current Microbiology - Tập 50 - Trang 155-159 - 2005
The ruminal bacterium, Prevotella bryantii B14, grew more rapidly with glucose as an energy source than mannose (0.73 versus 0.47 h−1) and had 8-fold less β-glucanase activity (50 versus 400 nmol reducing sugar mg protein−1 min−1). Cultures that were provided with glucose and mannose had little β-glucanase activity even though both sugars were utilized simultaneously. The observation that glucose ......
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