Current Microbiology
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Production of Volatile Compounds by a Variety of Fungi in Artificially Inoculated and Naturally Infected Aquilaria malaccensis
Current Microbiology - Tập 79 - Trang 1-14 - 2022
Aquilaria malaccensis, the resinous agarwood, is highly valued in the perfumery and medicinal industry. The formation of fragrant agarwood resin inconsistently by various fungi is still not clearly understood. The current study investigated the agarwood quality and fungal diversity in artificially inoculated and naturally infected A. malaccensis. The chemical analysis of volatile compounds of agar......
Diversity of Lactic Acid Bacteria Associated with Fresh Coffee Cherries in Taiwan
Current Microbiology - Tập 68 - Trang 440-447 - 2013
A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and
Weissella species were the most common LAB found in two farms located at an approximate altitude of 800 m. ......
Effects of Green Manures on Rhizosphere Fungal Community Composition of Cucumber Seedlings
Current Microbiology - Tập 80 - Trang 1-7 - 2023
Green manures are usually used to improve soil health and increase crop productivity. The activity and composition of the soil microbial community could be altered by green manures. High-throughput amplicon sequencing was used to assess the effects of green manures of wheat (Triticum aestivum) and Indian mustard (Brassica juncea) on the rhizosphere fungal community composition of cucumber (Cucumis......
Halomonas humidisoli Sp. Nov., Isolated From Saline–Alkaline Soil
Current Microbiology - Tập 78 - Trang 803-809 - 2021
A Gram staining-negative, halophilic, aerobic, non-motile bacteria, designated strain WN018T, were isolated from the natural saline–alkali wetland soil of Binhai new district, Tianjin, China (38°46′N, 117°13′E). Cells of strain WN018T were short rod-shaped, 0.3–0.4 µm wide and 0.5–1.9 µm long, and growth occurred optimally at 30–33 °C, pH 7.5–8.0, and in the presence of 4–8% (w/v) NaCl. Based on 1......
Bluetongue virus: Detection of antiviral immunoglobulin G by means of enzyme-linked immunosorbent assay
Current Microbiology - Tập 4 - Trang 381-385 - 1980
An enzyme-linked immunosorbent assay was developed to detect antiviral IgG in the sere of sheep exposed to bluetongue virus. It was found that the enzyme-linked immunosorbent assay is a rapid and sensitive method for the detection of anti-bluetongue virus antibody. Bluetongue virus antigen prepared from extracts of virus infected BHK and Vero cells were equally effective. Antigen prepared from uni......
Long-Term Survival of Bacillus Spores in Alcohol and Identification of 90% Ethanol as Relatively More Spori/Bactericidal
Current Microbiology - Tập 64 - Trang 130-139 - 2011
This study was taken up with a view to generate basic information on spore hardiness to ethanol in various Bacillus species and related genera, and to assess the effectiveness of different levels of ethanol as a bacterial disinfectant. Predominantly spore-bearing cultures of five Bacillus spp. (B. pumilus, B. subtilis, B. megaterium, B. fusiformis and B. flexus) that were isolated from the spent-a......
SigB-Dependent Tolerance to Protein Synthesis-Inhibiting Antibiotics in Listeria monocytogenes EGDe
Current Microbiology - Tập 64 - Trang 234-241 - 2011
The alternative sigma factor SigB in food-borne pathogen Listeria monocytogenes was determined in this study to be required for tolerance to protein synthesis-inhibiting antibiotics. The minimum inhibitory concentrations of tetracycline HCl and gentamicin sulphate against EGDeΔsigB were two- and fourfold less than those for EGDe, respectively. The ability of EGDeΔsigB to overcome the growth arrest......
Purification and Characterization of an Arylamine N-Acetyltransferase from the Bacteria Aeromonas hydrophilia
Current Microbiology - Tập 37 - Trang 70-73 - 1998
N-acetyltransferase from Aeromonas hydrophilia was purified by ultrafiltration, DEAE-Sephacel, gel filtration chromatography on Sephadex G-100, and DEAE-5pw on high performance liquid chromatography, as judged by sodium dodecyl sulfate-polyacrylamine gel electrophoresis (SDS-PAGE) on a 12.% (wt/vol) slab gel. The enzyme had a molecular mass 44.9 kDa. The purified enzyme was thermostable at 37°C f......
Corallibacterium pacifica gen. nov., sp. nov., a Novel Bacterium of the Family Vibrionaceae Isolated from Hard Coral
Current Microbiology - Tập 75 - Trang 835-841 - 2018
A gram-negative, rod-shaped, motile, oxidase- and catalase-positive, non-pigmented marine bacterium, designated strain OS-11M-2T, was isolated from a coral sample collected from the Osakura coastal area in Micronesia. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences indicated that strain OS-11M-2T is a member of the family Vibrionaceae, its closest neighbors being Photobacter......
Flow-Cytometric Method for Viability Analysis of Mycoplasma gallisepticum and Other Cell-Culture-Contaminant Mollicutes
Current Microbiology - Tập 78 - Trang 67-77 - 2020
Mycoplasma is the smallest self-replicating bacteria, figuring as common contaminant of eukaryotic cell cultures. Production inputs and operator’s manipulation seem to be the main sources of such contamination. Many analytical approaches have been applied for mycoplasma detection in cell cultures and also in biological products. However, unless they were validated, only indicator cell culture and ......
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