Thermophilic methanogenesis from pectin by a mixed defined bacterial culture

Bernard Ollivier1, Jean-Louis Garcia1
1Laboratoire de Microbiologie ORSTOM, Université de Provence, Marseille, France

Tóm tắt

Thermophilic degradation of pectin was studied in batch cultures at 55°C by different associations of anaerobic bacteria, includingClostridium thermocellum, Methanobacterium sp., andMethanosarcina sp.Clostridium thermocellum alone produced large amounts of methanol along with some isopropanol and H2. The inoculation ofMethanobacterium sp. in the culture did not affect the metabolism ofC. thermocellum; this demonstrates the absence of interspecies hydrogen transfer. In the presence of the methylotrophicMethanosarcina sp., methanol was reduced to methane without effect on pectin hydrolysis; a small amount of the H2 produced was also used to reduce methanol.

Từ khóa


Tài liệu tham khảo

Avrova NP, Zubko IK, Alekseeva EG (1981) Fermentation products and pectinolytic enzyme activity ofClostridium felsineum strains having different rates of spore germination. Microbiologiya 3:318–325

Breure AM, Beerepoot M, Van Andel JG (1985) Acidogenic fermentation of pectin by a mixed population of bacteria in continuous culture. Biotechnol Lett 7:341–344

Cato EP, George WL, Finegold SM (1986) GenusClostridium. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds) Bergey's Manual of systematic bacteriology, vol. 2. Baltimore: Williams & Wilkins, pp 1141–1200

Fogarty WM, Ward OP (1974) Pectinases and pectic poly-saccharides. Progr Ind Microbiol 13:59–119

Garcia JL, Guyot JP, Ollivier B, Trad M, Paycheng C (1982) Ecologie microbienne de la digestion anaérobie: techniques de numération et d'isolement. Cah ORSTOM, sér Biol 45:3–15

Hollaus F, Sleytr U (1972) On the taxonomy and fine structure of some hyperthermophilic saccharolytic clostridia. Ark Mikrobiol 86:129–146

Hungate RE (1969) A roll-tube method for cultivation of strict anaerobes. In: Norris R, Ribbons DW (eds) Methods in microbiology, vol. 3B. New York: Academic Press, pp 117–132

Lund BM, Brocklehurst JF (1978) Pectic enzymes of pigmentated strains ofClostridium. J. Gen Microbiol 104:59–66

Macy JM, Snellen JE, Hungate RE (1972) Use of syringe methods for anaerobiosis. Am J Clin Nutr 25:1318–1323

Ollivier B, Lombardo A, Garcia JL (1984) Isolation and characterization of a new thermophilicMethanosarcina strain (strain MP). Ann Microbiol (Inst Pasteur) 135B:187–198

Ollivier B, Smiti N, Garcia JL (1985) Thermophilic methanogenesis from sugar beet pulp by a defined mixed bacterial culture. Biotechnol Lett 7:847–852

Ollivier B, Smiti N, Mah RA, Garcia JL (1986) Thermophilic methanogenesis from gelatin by a mixed defined bacterial culture. Appl Microbiol Biotechnol 24:79–83

Rexova-Benkova L, Marokovic O (1976) Pectic enzymes. Adv Carbohydr Chem 33:323–385

Rhode LM, Sharak Genthner BR, Bryant MP (1981) Syntrophic association by cocultures of methanol- and CO2-H2-utilizing speciesEubacterium limosum and pectin-fermentingLachnospira multiparus during growth in a pectin medium. Appl Environ Microbiol 42:20–22

Rombouts FM, Pilnik W (1980) Pectic enzymes. In: Rose AH (ed) Economy microbiology, vol. 5, Microbial enzymes and bioconversion. London: Academic Press, pp 227–282

Schink B, Zeikus JG (1980) Microbial methanol formation: a major end product of pectin metabolism. Curr Microbiol 4:387–389

Schink B, Zeikus JG (1982) Microbial ecology of pectin decomposition in anoxic lake sediments. J Gen Microbiol 128:393–404

Schink B, Zeikus JG (1983)Clostridium thermosulfurogenes sp. nov., a new thermophile that produces elemental sulphur from thiosulphate. J Gen Microbiol 129:1149–1158

Schink, B, Zeikus JG (1983) Characterization of pectinolytic enzymes ofClostridium thermosulfurogenes. FEMS Microbiol Lett 17:295–298

Schink B, Ward JC, Zeikus JG (1981) Microbiology of wetwood: importance of protein degradation andClostridium species in living trees. Appl Environ Microbiol 42:526–532

Sheiman MI, MacMillan JO, Miller L, Chase JR (1976) Coordinated action of pectinesterase and polygalacturonase lyase complex ofClostridium multifermentans. Eur J Biochem 64:565–572

Smiti N, Ollivier B, Garcia JL (1986) Thermophilic degradation of cellulose by a triculture ofClostridium thermocellum, Methanobacterium sp. andMethanosarcina MP. FEMS Microbiol Lett 35:93–97

Somogyi LP, Romani R (1964) A simplified technique for the determination of pectin methylesterase activity. Anal Biochem 7:498–501

Spinnler HE, Lavigne B, Blachere H (1986) Pectinolytic activity ofClostridium thermocellum: its use for anaerobic fermentation of sugar beet pulp. Appl Microbiol Biotechnol 23:434–437

Szymanski PT (1981) A note on the fermentation of pectin by pure strains and combined cultures of rumen bacteria. Acta Microbiol Pol 30:159–163

Weimer PJ, Zeikus JG (1977) Fermentation of cellulose and cellobiose byClostridium thermocellum in the absence and presence ofMethanobacterium thermoautotrophicum. Appl Environ Microbiol 33:289–297

Wilkinson JF, Rose AH (1963) Fermentation processes. In: Rainbow C, Rose AH (eds) Biochemistry of industrial micro-organisms. London: Academic Press, pp 379–414

Wojciechowitz M, Tomerska H (1971) Pectic enzymes in some pectinolytic rumen bacteria. Acta Microbiol Pol Ser. A3:57–61

Zinder SH, Mah RA (1979) Isolation and characterization of a thermophilic strain ofMethanosarcina unable to use H2-CO2 for methanogenesis. Appl Environ Microbiol 38:996–1008