Development and characterization of microsatellite markers in Schizopyge niger (Family: Cyprinidae, Pisces) from Kashmir ValleyConservation Genetics Resources - Tập 5 - Trang 831-833 - 2013
Ashoktaru Barat, Rakesh Matura, Jyoti Sati, Rohit Kumar, Farroz A. Bhat, Chirag Goel, Prabhati Kumari Sahoo
A set of 8 microsatellites was developed for a freshwater fish chush snowtrout, Schizopyge niger. Chush snowtrout is an endemic fish to Kashmir valley, India. Constructing a partial genomic library, we obtained 36 clones containing putative microsatellite motifs and 12 microsatellite with enough flanking sequence for primer designing. The number of alleles per locus ranged from 3 to 7 (mean 4.75),...... hiện toàn bộ
Isolation and characterization of microsatellite loci from Gasterophilus pecorum (Diptera: Gasterophilidae)Conservation Genetics Resources - Tập 6 - Trang 487-489 - 2014
Fang Cheng, Jianmei Yang, Dong Zhang, Defu Hu, Hongjun Chu, Jie Cao, Feng Jiang, Lan Bu, Kai Li
Gasterophilus pecorum (Diptera: Gasterophilidae) is the most damaging parasites in Przewalaski’s horses that were re-wilded in Kalamaili, Xinjiang, China. In this study, 18 polymorphic microsatellite loci were isolated for the first time from G. pecorum. The number of alleles per locus ranged from 3 to 19 and the mean number of alleles was 10.78. Mean observed and expected heteroz...... hiện toàn bộ
An improved PCR-based method for faster sex determination in brown trout (Salmo trutta) and Atlantic salmon (Salmo salar)Conservation Genetics Resources - Tập 6 - Trang 825-827 - 2014
Erwan Quéméré, Charles Perrier, Anne-Laure Besnard, Guillaume Evanno, Jean-Luc Baglinière, Yann Guiguen, Sophie Launey
Rapid and accurate sex-identification of salmonids has remained an important challenge for aquaculture and wild population study and management. A molecular method looking for the presence of the male-specific sdY gene has recently been proposed. However, the size of the amplified fragment (~500pb) and the DNA revealing technique (agarose-gel electrophoresis) preclude its use on partially degraded...... hiện toàn bộ
Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)Conservation Genetics Resources - Tập 2 - Trang 279-282 - 2010
Alison J. Peel, Stephen J. Rossiter, James L. N. Wood, Andrew A. Cunningham, David R. Sargan
Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3–46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to...... hiện toàn bộ
A novel and diverse set of SNP markers for rangewide genetic studies in Picea abiesConservation Genetics Resources - Tập 14 - Trang 267-270 - 2022
Malte Mader, Céline Blanc-Jolivet, Birgit Kersten, Heike Liesebach, Bernd Degen
We used Double Digest Restriction site associated DNA sequencing (ddRADseq), exome sequencing (exome-seq) and targeted genotyping by sequencing (GBS) to develop new geographically informative nuclear SNP markers in Picea abies. This set of 518 loci consists of 397 loci specifically designed for the geographic differentiation of populations and 121 loci of adaptive markers for drought stress which ...... hiện toàn bộ
Isolation and characterization of microsatellite markers from the camouflage grouper, Epinephelus polyphekadion (Epinephelidae)Conservation Genetics Resources - Tập 5 - Trang 1129-1132 - 2013
Ka Yan Ma, Yvonne Sadovy de Mitcheson, Ka Hou Chu
We report the isolation and development of 12 novel microsatellite loci for the reef-associated camouflage grouper, Epinephelus polyphekadion, a near-threatened grouper which is widely distributed, little managed, and heavily fished throughout the Indo-Pacific region. The number of alleles ranged from two to 11. No evidence of linkage disequilibrium was found among loci, but two of the loci showed...... hiện toàn bộ
An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic catsConservation Genetics Resources - Tập 4 - Trang 163-165 - 2011
Ross McEwing, Andrew C. Kitchener, Clare Holleley, Kerry Kilshaw, Paul O’Donoghue
Here we present an allelic discrimination assay designed to distinguish European wildcat mtDNA lineages from those of the domestic cat. Introgression between the native wildcat and introduced domestic cat has the potential to limit and reduce the recent recovery of remnant populations. Applied conservation genetic techniques can aid current conservation decisions on lethal control and neutering m...... hiện toàn bộ