Development and characterization of 13 novel microsatellite loci in a threatened reef fish, humpback grouper (Cromileptes altivelis)

Conservation Genetics Resources - Tập 6 - Trang 781-782 - 2014
Yu Wang1, Guohua Chen1, Feng Yan1, Haoran Lin1,2, Jian Luo1
1Key Laboratory of Tropical Biological Resources of Ministry of Education, Ocean College, Hainan University, Haikou, People’s Republic of China
2State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen University, Guangzhou, People’s Republic of China

Tóm tắt

Cromileptes altivelis is a commercially important and vulnerable reef fish in Southeast Asia. 13 microsatellite loci were isolated and characterized from 37 individuals collected in the South China Sea. The allele numbers per locus ranged from 2 to 7 and the observed and expected heterozygosity ranged from 0.135 to 0.730 and from 0.196 to 0.764, respectively. These molecular markers can be used for the development of genomic databases and population genetic studies, the results of which will inform conservation management.

Tài liệu tham khảo

Moraga AD, Farmerie B, Holland K, Blake A et al (1998) Tools for developing molecular markers: microsatellite libraries and AFLPS. University of Florida, Gainesville, FL, Workshop Manual Qin J, Hu D, Yang W, Xiao J (2014) Complete mitochondrial genome of the hunmpback grouper Cromileptes altivelis. Mitochondrial DNA 25(3):200–201 Rodrigues KF, Shigeharu S et al (2011) Microsatellite markers for the identification of commercially important groupers Epinephelus lanceolatus, Cromileptes altivelis and Epinephelus fuscoguttatus. Pertanika J Trop Agric Sci 34(2):311–315 Yeh F, Yang RC, Boyle T (1999) POPGEN v3. 2, http://www.ualberta.ca/~fyeh/download.htm
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Conservation Genetics Resources

Tập 6

781-782

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