Biotechnology for Biofuels and Bioproducts
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Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-13 - 2022
Production of vitamin C has been traditionally based on the Reichstein process
and the two-step process. However, the two processes share a common
disadvantage: vitamin C cannot be directly synthesized from D-glucose.
Therefore, significant effort has been made to develop a one-step vitamin C
fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly
all current one-step ferment...
Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-16 - 2022
As an attractive platform chemical, malic acid has been commonly used in the
food, feed and pharmaceutical field. Microbial fermentation of biobased sources
to produce malic acid has attracted great attention because it is sustainable
and environment-friendly. However, most studies mainly focus on improving yield
and ignore shortening fermentation time. A long fermentation period means high
cost, ...
Oleaginous yeast, Rhodotorula paludigena CM33, platform for bio-oil and biochar productions via fast pyrolysis
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-14 - 2023
An oleaginous yeast Rhodotorula paludigena CM33 was pyrolyzed for the first time
to produce bio-oil and biochar applying a bench-scale reactor. The strain
possessed a high lipid content with the main fatty acids similar to vegetable
oils. Prior to pyrolysis, the yeast was dehydrated using a spray dryer.
Pyrolysis temperatures in the range of 400–600 °C were explored in order to
obtain the optimal ...
Lignin bioconversion based on genome mining for ligninolytic genes in Erwinia billingiae QL-Z3
Biotechnology for Biofuels and Bioproducts - Tập 17 - Trang 1-18 - 2024
Bioconversion of plant biomass into biofuels and bio-products produces large
amounts of lignin. The aromatic biopolymers need to be degraded before being
converted into value-added bio-products. Microbes can be environment-friendly
and efficiently degrade lignin. Compared to fungi, bacteria have some advantages
in lignin degradation, including broad tolerance to pH, temperature, and oxygen
and the...
Development of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-13 - 2023
The production of bio-succinic acid (SA) from renewable feedstocks is a
promising and sustainable approach to mitigating the high carbon emissions
associated with the current energy crisis. Actinobacillus succinogenes was
recognized as one of the most promising SA producers; however, lack of genetic
background and the scarcity of genetic manipulation tools hinder the improvement
in A. succinogenes...
Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-17 - 2023
Harnessing engineered Mycolicibacteria to convert cheap phytosterols into
valuable steroid synthons is a basic way in the industry for the production of
steroid hormones. Thus, C-19 and C-22 steroids are the two main types of
commercial synthons and the products of C17 side chain degradation of
phytosterols. During the conversion process of sterols, C-19 and C-22 steroids
are often produced togeth...
Structure and function of aldopentose catabolism enzymes involved in oxidative non-phosphorylative pathways
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-21 - 2022
Platform chemicals and polymer precursors can be produced via enzymatic pathways
starting from lignocellulosic waste materials. The hemicellulose fraction of
lignocellulose contains aldopentose sugars, such as d-xylose and l-arabinose,
which can be enzymatically converted into various biobased products by microbial
non-phosphorylated oxidative pathways. The Weimberg and Dahms pathways convert
pent...
Exploring the cellulolytic and hemicellulolytic activities of manganese peroxidase for lignocellulose deconstruction
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-10 - 2023
A cost-effective pretreatment and saccharification process is a necessary
prerequisite for utilizing lignocellulosic biomass (LCB) in biofuel and
biomaterials production. Utilizing a multifunctional enzyme with both
pretreatment and saccharification functions in a single step for simultaneous
biological pretreatment and saccharification process (SPS) will be a green
method of low cost and high eff...
Multi-step biosynthesis of the biodegradable polyester monomer 2-pyrone-4,6-dicarboxylic acid from glucose
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-12 - 2023
2-Pyrone-4,6-dicarboxylic acid (PDC), a chemically stable pseudoaromatic
dicarboxylic acid, represents a promising building block for the manufacture of
biodegradable polyesters. Microbial production of PDC has been extensively
investigated, but low titers and yields have limited industrial applications. In
this study, a multi-step biosynthesis strategy for the microbial production of
PDC was demo...
Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-24 - 2023
The efficient production of sustainable biofuels is important for the reduction
of greenhouse gas emissions. Clostridium thermocellum ATCC 27405 is a candidate
for ethanol production from lignocellulosic biomass using consolidated
bioprocessing. Fermentation of cellulosic biomass goes through an atypical
glycolytic pathway in this thermophilic bacterium, with various glycolytic
enzymes capable of ...
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