Biotechnology for Biofuels and Bioproducts
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Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-13 - 2022
Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-glucose. Therefore, significant effort has been made to develop a one-step vitamin C fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly all current one-step ferment......
Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-16 - 2022
As an attractive platform chemical, malic acid has been commonly used in the food, feed and pharmaceutical field. Microbial fermentation of biobased sources to produce malic acid has attracted great attention because it is sustainable and environment-friendly. However, most studies mainly focus on improving yield and ignore shortening fermentation time. A long fermentation period means high cost, ......
Oleaginous yeast, Rhodotorula paludigena CM33, platform for bio-oil and biochar productions via fast pyrolysis
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-14 - 2023
An oleaginous yeast Rhodotorula paludigena CM33 was pyrolyzed for the first time to produce bio-oil and biochar applying a bench-scale reactor. The strain possessed a high lipid content with the main fatty acids similar to vegetable oils. Prior to pyrolysis, the yeast was dehydrated using a spray dryer. Pyrolysis temperatures in the range of 400–600 °C were explored in order to obtain the optimal ......
Lignin bioconversion based on genome mining for ligninolytic genes in Erwinia billingiae QL-Z3
Biotechnology for Biofuels and Bioproducts - Tập 17 - Trang 1-18 - 2024
Bioconversion of plant biomass into biofuels and bio-products produces large amounts of lignin. The aromatic biopolymers need to be degraded before being converted into value-added bio-products. Microbes can be environment-friendly and efficiently degrade lignin. Compared to fungi, bacteria have some advantages in lignin degradation, including broad tolerance to pH, temperature, and oxygen and the......
Development of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-13 - 2023
The production of bio-succinic acid (SA) from renewable feedstocks is a promising and sustainable approach to mitigating the high carbon emissions associated with the current energy crisis. Actinobacillus succinogenes was recognized as one of the most promising SA producers; however, lack of genetic background and the scarcity of genetic manipulation tools hinder the improvement in A. succinogenes......
Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-17 - 2023
Harnessing engineered Mycolicibacteria to convert cheap phytosterols into valuable steroid synthons is a basic way in the industry for the production of steroid hormones. Thus, C-19 and C-22 steroids are the two main types of commercial synthons and the products of C17 side chain degradation of phytosterols. During the conversion process of sterols, C-19 and C-22 steroids are often produced togeth......
Structure and function of aldopentose catabolism enzymes involved in oxidative non-phosphorylative pathways
Biotechnology for Biofuels and Bioproducts - Tập 15 - Trang 1-21 - 2022
Platform chemicals and polymer precursors can be produced via enzymatic pathways starting from lignocellulosic waste materials. The hemicellulose fraction of lignocellulose contains aldopentose sugars, such as d-xylose and l-arabinose, which can be enzymatically converted into various biobased products by microbial non-phosphorylated oxidative pathways. The Weimberg and Dahms pathways convert pent......
Exploring the cellulolytic and hemicellulolytic activities of manganese peroxidase for lignocellulose deconstruction
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-10 - 2023
A cost-effective pretreatment and saccharification process is a necessary prerequisite for utilizing lignocellulosic biomass (LCB) in biofuel and biomaterials production. Utilizing a multifunctional enzyme with both pretreatment and saccharification functions in a single step for simultaneous biological pretreatment and saccharification process (SPS) will be a green method of low cost and high eff......
Multi-step biosynthesis of the biodegradable polyester monomer 2-pyrone-4,6-dicarboxylic acid from glucose
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-12 - 2023
2-Pyrone-4,6-dicarboxylic acid (PDC), a chemically stable pseudoaromatic dicarboxylic acid, represents a promising building block for the manufacture of biodegradable polyesters. Microbial production of PDC has been extensively investigated, but low titers and yields have limited industrial applications. In this study, a multi-step biosynthesis strategy for the microbial production of PDC was demo......
Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles
Biotechnology for Biofuels and Bioproducts - Tập 16 - Trang 1-24 - 2023
The efficient production of sustainable biofuels is important for the reduction of greenhouse gas emissions. Clostridium thermocellum ATCC 27405 is a candidate for ethanol production from lignocellulosic biomass using consolidated bioprocessing. Fermentation of cellulosic biomass goes through an atypical glycolytic pathway in this thermophilic bacterium, with various glycolytic enzymes capable of ......
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