Biotechnology Letters
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The development of transgenic mice for the expression of large amounts of human lysozyme in milk
Biotechnology Letters - - 2014
N-alkane biodegradation by a marine bacterium in the presence of an oleophilic nutriment
Biotechnology Letters - Tập 15 - Trang 637-640 - 1993
Hexadecane biodegradation by a marine bacterium has been investigated in the presence of an oleophilic nutriment (INIPOL EAP 22). Hydrocarbon attack was only observed after metabolism of the fatty acids present in the fertilizer. The bacterium used up 95 % fatty acids in the first 24 hours. Hexadecane biodegradation took place after 50 h incubation and reached 40 % after 360 h.
Regioselective enzymatic acylation of ribavirin to give potential multifunctional derivatives
Biotechnology Letters - Tập 27 - Trang 717-720 - 2005
Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was regioselectivly acylated at the primary hydroxyl groups and the corresponding viny......
HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis
Biotechnology Letters - Tập 40 - Trang 623-631 - 2018
To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung cancer cells. Importantly, HuR and CDK3 expressions were increased in lung cancer tissues compared with ......
Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion
Biotechnology Letters - Tập 39 - Trang 1747-1755 - 2017
To carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant. The first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a ......
Immobilization of naringinase on glycophase-coated porous glass
Biotechnology Letters - Tập 7 - Trang 477-482 - 1985
Naringinase from Penicillium sp. was covalently linked to Glycophasecoated controlled-pore glass. The parameters of the immobilization process were characterized with respect to both the coupling method as well as to support pore size. The efficient kinetic parameters shown by the most active and stable derivative enables it to be used for debittering of naringin-containing juices.
Linear dynamic range for signal detection in fluorescent differential display
Biotechnology Letters - Tập 32 - Trang 1593-1597 - 2010
We have determined the linear dynamic range in signal detection by Fluorescent Differential Display (FDD) using conditionally induced mRNA expression of the p53 tumor-suppressor gene as a control. By serial spiking of p53-induced RNA into that of non-induced RNA, we were able to quantitatively measure up to 100-fold change in p53 mRNA expression level. The linear dynamic range of signal detection ......
Effect of Trichoplusia ni BTI-Tn 5B1-4 cell density on human secreted alkaline phosphatase production
Biotechnology Letters - Tập 15 - Trang 1007-1012 - 1993
The effect of Tn 5B1-4 cell density at infection on the production of human secreted alkaline phosphatase (SEAP) was investigated using a split-flow air-lift bioreactor. Volumetric productivities of SEAP were highest when Tn 5B1-4 cells were infected at about 3 × 105 cells/cm2. Cell-to-cell contact inhibition is an important factor responsible for the reduced protein production at high cell densit......
Potenytial cost savings for fuel ethanol production by employing a novel hybrid yeast strain
Biotechnology Letters - Tập 7 - Trang 349-354 - 1985
A hybrid yeast (Labatt culture collection strain 1393) was investigated for its ability to ferment a corn mash with reduced concentrations of added glucoamylase. It was found that glucoamylase additions could be decreased by as much as 50 percent. This reduction could represent significant cost savings in the production of fuel ethanol.
Survival of nisin activity in intestinal environment
Biotechnology Letters - Tập 31 Số 8 - Trang 1229-1232 - 2009
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