Biotechnology Letters
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N-alkane biodegradation by a marine bacterium in the presence of an oleophilic nutriment
Biotechnology Letters - Tập 15 - Trang 637-640 - 1993
Hexadecane biodegradation by a marine bacterium has been investigated in the presence of an oleophilic nutriment (INIPOL EAP 22). Hydrocarbon attack was only observed after metabolism of the fatty acids present in the fertilizer. The bacterium used up 95 % fatty acids in the first 24 hours. Hexadecane biodegradation took place after 50 h incubation and reached 40 % after 360 h.
HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis
Biotechnology Letters - Tập 40 - Trang 623-631 - 2018
To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung cancer cells. Importantly, HuR and CDK3 expressions were increased in lung cancer tissues compared with ......
Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion
Biotechnology Letters - Tập 39 - Trang 1747-1755 - 2017
To carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant. The first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a ......
Linear dynamic range for signal detection in fluorescent differential display
Biotechnology Letters - Tập 32 - Trang 1593-1597 - 2010
We have determined the linear dynamic range in signal detection by Fluorescent Differential Display (FDD) using conditionally induced mRNA expression of the p53 tumor-suppressor gene as a control. By serial spiking of p53-induced RNA into that of non-induced RNA, we were able to quantitatively measure up to 100-fold change in p53 mRNA expression level. The linear dynamic range of signal detection ......
Survival of nisin activity in intestinal environment
Biotechnology Letters - Tập 31 Số 8 - Trang 1229-1232 - 2009
Protease-resistant core form of Bacillus thuringiensis Cry1Ie: monomeric and oligomeric forms in solution
Biotechnology Letters - Tập 31 - Trang 1769-1774 - 2009
Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantit......
A parallel bubble column system for the cultivation of phototrophic microorganisms
Biotechnology Letters - Tập 30 - Trang 1197-1200 - 2008
An incubator with up to 16 parallel bubble columns was equipped with artificial light sources assuring a light supply with a homogenous light spectrum directly above the bioreactors. Cylindrical light reflecting tubes were positioned around every single bubble column to avoid light scattering effects and to redirect the light from the top onto the cylindrical outer glass surface of each bubble col......
Plant growth-promoting bacterium Pseudomonas fluorescens FR1 secrets a novel type of extracellular polyhydroxybutyrate polymerase involved in abiotic stress response in plants
Biotechnology Letters - Tập 40 - Trang 1419-1423 - 2018
Identification of novel microbial factors contributing to plant protection against abiotic stress. The genome of plant growth-promoting bacterium Pseudomonas fluorescens FR1 contains a short mobile element encoding a novel type of extracellular polyhydroxybutyrate (PHB) polymerase (PhbC) associated with a type I secretion system. Genetic analysis using a phbC mutant strain and plants showed that t......
Enhancement of the thermostability of a recombinant β-agarase, AgaB, from Zobellia galactanivorans by random mutagenesis
Biotechnology Letters - Tập 32 - Trang 943-949 - 2010
Random mutagenesis was performed on β-agarase, AgaB, from Zobellia galactanivorans to improve its catalytic activity and thermostability. The activities of three mutants E99K, T307I and E99K–T307I were approx. 140, 190 and 200%, respectively, of wild type β-agarase (661 U/mg) at 40°C. All three mutant enzymes were stable up to 50°C and E99K–T307I had the highest thermostability. The melting temper......
Construction and characterization of a linked-dimeric pyrroloquinoline quinone glucose dehydrogenase
Biotechnology Letters - - 1999
Using the structural gene of a homo dimeric enzyme, the water-soluble pyrroloquinoline quinone glucose dehydrogenase (PQQGDH-B), a gene consisting of two identical subunits linked together by a DNA segment coding linker peptide region was constructed. Using the constructed gene, a linked-dimeric PQQGDH-B was produced in Escherichia coli as the active soluble enzyme. Linked-dimeric PQQGDH-B showed ......
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