Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion

Biotechnology Letters - Tập 39 - Trang 1747-1755 - 2017
Morgan E. Shires1, Sergio L. Florez1, Tina S. Lai1, Wayne R. Curtis1
1Department of Chemical Engineering, The Pennsylvania State University, University Park, USA

Tóm tắt

To carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant. The first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a dexamethasone activatable chimeric transcription factor. This four-fold enhancement in embryo production rate utilized a glucocorticoid receptor fused to an embryogenic transcription factor LEAFY COTYLEDON 2. Where previous T. cacao somatic embryogenesis has been restricted to dissected flower parts, this construct confers an unprecedented embryogenic potential to leaves. Activatable chimeric transcription factors provide a means for elucidating the regulatory cascade associated with plant somatic embryogenesis towards improving its use for somatic regeneration of transgenics and plant propagation.

Tài liệu tham khảo

Argout X, Salse J, Aury J-M, Guiltinan MJ et al (2011) The genome of Theobroma cacao. Nat Genet 43:101–108

Asplund PT, Curtis WR (2001) Intrinsic oxygen use kinetics of transformed plant root culture. Biotechnol Prog 17:481–489

Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic (Amsterdam) 122:507–520

Report: MarketsandMarkets.com (2011) Global chocolate, cocoa beans, lecithin, sugar and vanilla market by market share, trade, prices, geography trend and forecast (2011–2016). Dallas, TX. http://www.marketsandmarkets.com/PressReleases/global-chocolate-market.asp

Ducos J-P, Lambot C, Pétiard V (2007) Bioreactors for coffee mass propagation by somatic embryogenesis. Int J Plant Dev Biol 1:1–12

Ducos J-P, Terrier B, Courtois D (2010) Disposable bioreactors for plant micropropagation and mass plant cell culture. Adv Biochem Eng/Biotechnol 115:89–115

Elhiti M, Stasolla C, Wang A (2013) Molecular regulation of plant somatic embryogenesis. Vitr Cell Dev Biol 49:631–642

Fehér A (2015) Somatic embryogenesis—stress-induced remodeling of plant cell fate. Biochem Biophys Acta 1849:385–402

Florez SL (2015) Improving plant propagation through the manipulation of the genetic and physical environment. Ph.D. Dissertation, Pennsylvania State University, USA

Florez SL, Curtis MS, Shaw SE, Hamaker NK, Larsen JS, Curtis WR (2015a) A temporary immersion plant propagation bioreactor with decoupled gas and liquid flows for enhanced control of gas phase. Biotechnol Prog 32:337–345

Florez SL, Erwin RL, Maximova SN, Guiltinan MJ, Curtis WR (2015b) Enhanced somatic embryogenesis in Theobroma cacao using the homologous BABY BOOM transcription factor. BMC Plant Biol 15:121. doi:10.1186/s12870-015-0479-4

Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool. Microbiol Mol Biol Rev 67:16–37

Gliwicka M, Nowak K, Balazadeh S, Mueller-Roeber B, Gaj MD (2013) Extensive modulation of the transcription factor transcriptome during somatic embryogenesis in Arabidopsis thaliana. PLoS ONE. doi:10.1371/journal.pone.0069261

Guiltinan MJ, Maximova SN (2010) Integrated system for vegetative propagation of cacao. The Pennsylvania State University. 26 pp

Gupta PK, Durzan DJ (1987) Biotechnology of somatic polyembryogenesis and plantlet regeneration in loblolly pine. Nat Biotechnol 5:147–151

Gupta P, Hartle J (2015) Progress on scale-up somatic embryogenesis and manufactued seed technology on conifer species at Weyerhauser. Vitr Cell Dev Biol 51:S13–S14

Kamle M, Bajpai A, Chandra R, Kalim S, Kumar R (2011) Somatic embryogenesis for crop improvement. GERF Bull BioscI 2:54–59

Khanday I, Das S, Chongloi GL, Bansal M, Grossniklaus U, Vijayraghavan U (2016) Genome-wide targets regulated by the OsMADS1 transcription factor reveals its DNA recognition properties. Plant Physiol 172:372–388

Kumar N, Reddy MP (2011) In vitro plant propagation: a review. For Sci 27:61–72

Li Z, Traore A, Maximova S, Guiltinan MJ (1998) Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron. Vitr Cell Dev Biol 34:293–299

Maximova S, Miller C, Antúnez de Mayolo G, Pishak S, Young A, Guiltinan MJ (2003) Stable transformation of Theobroma cacao L. and influence of matrix attachment regions on GFP expression. Plant Cell Rep 21:872–883

Schena M, Lloyd AM, Davis RW (1991) A steroid-inducible gene expression system for plant cells. Proc Natl Acad Sci USA 88:10421–10425

Stone SL, Braybrook SA, Paula SL, Kwong LW, Meuser J, Pelletier J, Hsieh T-F, Fischer RL, Goldberg RB, Harada JJ (2008) Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: implications for somatic embryogenesis. Proc Natl Acad Sci USA 105:3151–3156

Tripathi JN, Muwonge A, Tripathi L (2012) Efficient regeneration and transformation of plantain cv. “Gonja manjaya” (Musa spp. AAB) using embryogenic cell suspensions. Vitr Cell Dev Biol 48:216–224

van der Linde K (1999) Improved bacteriological surveillance of haemodialysis fluids: a comparison between Tryptic soy agar and Reasoner’s 2A media. Nephrol Dial Transpl 14:2433–2437

Zhang Y, Clemens A, Maximova S, Guiltinan M (2014) The Theobroma cacao B3 domain transcription factor TcLEC2 plays a duel role in control of embryo development and maturation. BMC Plant Biol 14:106. doi:10.1186/1471-2229-14-106

Lutz KA, Martin C, Khairzada S, Maliga P (2015) Steriod-inducible BABY BOOM system for development of fertile Arabidopsis thaliana plants after prolonged tissue culture. Plant Cell Rep. 34:1849-1856

Passarinho P, Ketelaar T, Xing M, van Arkel J, Maliepaard C, Hendriks MW, Joosen R, Lammers M, Herdies L, den Boer B, van der Geest L, Boutelier K (2008) BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways. Plant Mol Biol. 68:225-237

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Biotechnology Letters

Tập 39

1747-1755

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