Bioprocess and Biosystems Engineering
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Experimental investigation of the adsorption and desorption of cellulase enzymes on zeolite-β for enzyme recycling applications
Bioprocess and Biosystems Engineering - Tập 44 - Trang 495-505 - 2020
The recyclability of cellulase enzymes using zeolite and polyethylene glycol (PEG) was investigated. The cellulase enzymes from cellulose hydrolysate suspensions were adsorbed onto zeolite-β under typical working conditions (pH 5). PEG having a molecular weight of 200 Da and 20 kDa was used as an eluent to desorb the cellulase enzymes from zeolite-β. Adsorption and desorption profiles of cellulase...... hiện toàn bộ
Emerging trends in environmental and industrial applications of marine carbonic anhydrase: a review
Bioprocess and Biosystems Engineering - - 2022
Physiological mechanism of the overproduction of ε-poly-l-lysine by acidic pH shock in fed-batch fermentation
Bioprocess and Biosystems Engineering - Tập 38 - Trang 2085-2094 - 2015
The introduction of an environmental stress of acidic pH shock had successfully solved the common deficiency existed in ε-PL production, viz. the distinct decline of ε-PL productivity in the feeding phase of the fed-batch fermentation. To unravel the underlying mechanism, we comparatively studied the physiological changes of Streptomyces sp. M-Z18 during fed-batch fermentations with the pH shock s...... hiện toàn bộ
Optimization of the unhairing leather processing with enzymes and the evaluation of inter-fibrillary proteins removal: an environment-friendly alternative
Bioprocess and Biosystems Engineering - - 2012
Preparation of lipase-surfactant complex for the catalysis of triglyceride hydrolysis in heterogeneous reaction systems
Bioprocess and Biosystems Engineering - Tập 15 - Trang 133-137 - 1996
To facilitate lipase (E.C.3.1.1.3) catalyzed hydrolysis reaction of triglycerides in heterogeneous reaction systems, lipase-surfactant complex (LSC) was prepared by mixing an aqueous solution of lipase and surfactant in ethanol. The LSC prepared from lipase MF-30 derived from Pseudomonas sp. and Nonion SP-60R (sorbitan mono-stearate) showed a high LSC yield and activity recovery. About 30% of the ...... hiện toàn bộ
Improvement of foam breaking and oxygen-transfer performance in a stirred-tank fermenter
Bioprocess and Biosystems Engineering - Tập 28 - Trang 235-242 - 2005
This study examined a stirred-tank fermenter (STF) containing low-viscosity foaming liquids with an agitation impeller and foam-breaking impeller mounted on the same shaft. Results showed that the performance of the foam-breaking impeller can be improved by changing a conventional six-blade turbine impeller into a rod impeller as the agitation impeller. The volumetric oxygen-transfer coefficient, ...... hiện toàn bộ
Optimization of culture medium for enhanced production of exopolysaccharide from Aureobasidium pullulans
Bioprocess and Biosystems Engineering - Tập 35 - Trang 167-172 - 2011
Polysaccharides produced by microorganisms are utilized for a variety of purposes, including the use in cosmetics and as food additives. More recently, polysaccharides have been exploited by the medical and pharmaceutical industries, and those originated from many species of mushrooms have been especially useful in industrial applications; however, the production and synthesis of these compounds i...... hiện toàn bộ
The effects of aeration and agitation on the measurement of yeast biomass using a laser turbidity probe
Bioprocess and Biosystems Engineering - Tập 16 - Trang 339-344 - 1997
The paper reports the measurement of biomass concentration using a laser turbidity probe. A suspension of Bakers’ yeast (0.5−50 gl-1) was subjected to various conditions of agitation and aeration in a stirred tank reactor and the turbidity measured using the probe. Both agitation and aeration were found to influence the turbidity. At any constant biomass concentration, the effect on the turbidity...... hiện toàn bộ
Production of monoglycerides by glycerolysis of olive oil with immobilized lipases: effect of the water activity
Bioprocess and Biosystems Engineering - Tập 12 - Trang 327-337 - 1995
The production of monoglycerides by glycerolysis of olive oil catalyzed by lipases from Candida rugosa, Chromobacterium viscosum and Rhizopus sp. immobilized in a hydrophylic polyurethane foam was investigated. The effect of the amount of aqueous phase used for foam polymerization on the competing reactions of glycerolysis and hydrolysis was studied. The highest monoglyceride production was achiev...... hiện toàn bộ
Improving lactate metabolism in an intensified CHO culture process: productivity and product quality considerations
Bioprocess and Biosystems Engineering - Tập 39 - Trang 1689-1702 - 2016
In this study, we discussed the development and optimization of an intensified CHO culture process, highlighting medium and control strategies to improve lactate metabolism. A few strategies, including supplementing glucose with other sugars (fructose, maltose, and galactose), controlling glucose level at <0.2 mM, and supplementing medium with copper sulfate, were found to be effective in reducing...... hiện toàn bộ
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