Applied Biochemistry and Biotechnology
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Application of Response Surface Methodology for Maximizing Dextransucrase Production from Leuconostoc mesenteroides NRRL B-640 in a Bioreactor
Applied Biochemistry and Biotechnology - Tập 151 - Trang 182-192 - 2008
The production of dextransucrase from Leuconostoc mesenteroides NRRL B-640 was investigated using statistical approaches. Plackett–Burman design with six variables, viz. sucrose, yeast extract, K2HPO4, peptone, beef extract, and Tween 80, was used to screen the nutrients that significantly affected the dextransucrase production. 24-Central composite design with four selected variables (sucrose, K2...... hiện toàn bộ
Studies on mass production of transformed Panax ginseng hairy roots in bioreactor
Applied Biochemistry and Biotechnology - Tập 98 - Trang 1115-1127 - 2002
The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased to 6.4. After 45 d, hairy roots grew about 16-folds. T...... hiện toàn bộ
Effect of nucleic acid contamination on partitioning of proteins in two-phase PEG-Dextran system
Applied Biochemistry and Biotechnology - Tập 54 - Trang 193-205 - 1995
Downstream processing of bioproducts results in considerable losses of compounds of interest in a large number of cases. For the intracellular enzyme tartrate dehydrogenase, an analysis of the laboratory process for enzyme recovery revealed that maximum losses occur in the initial stages of purification when the enzyme is separated from nucleic acids and other undesirable enzymes. Hence, aqueous t...... hiện toàn bộ
A colorimetric formaldehyde assay
Applied Biochemistry and Biotechnology - Tập 68 - Trang 81-93 - 1997
A room-temperature assay of formaldehyde is described. The assay uses few reagents and is colorimetric, read at a wavelength of 649 nm. Tryptophan and tryptamine were noted as interfering with the assay, probably by binding with the formaldehyde. High levels of sugar show smaller effects on final absorbance. Glyceraldehyde also reacts in the assay, but six other aldehyde compounds do not, although...... hiện toàn bộ
A magnetizable solid phase for enzyme extraction
Applied Biochemistry and Biotechnology - Tập 30 - Trang 155-164 - 1991
A method for the convenient and reliable preparation of magnetizable agarose beads containing iron particles is described. The beads were treated with the triazine dye, Reactive Red 120, and the matrix was examined for the ability to extract proteins from crude preparations using lactate dehydrogenase from porcine muscle as a model. The recovery and specific activity values of enzyme obtained usin...... hiện toàn bộ
Heavy Metal Resistances and Chromium Removal of a Novel Cr(VI)-Reducing Pseudomonad Strain Isolated from Circulating Cooling Water of Iron and Steel Plant
Applied Biochemistry and Biotechnology - Tập 180 - Trang 1328-1344 - 2016
Three bacterial isolates, GT2, GT3, and GT7, were isolated from the sludge and water of a circulating cooling system of iron and steel plant by screening on Cr(VI)-containing plates. Three isolates were characterized as the members of the genus Pseudomonas on the basis of phenotypic characteristics and 16S rRNA sequence analysis. All isolates were capable of resisting multiple antibiotics and heav...... hiện toàn bộ
C–S Targeted Biodegradation of Dibenzothiophene by Stenotrophomonas sp. NISOC-04
Applied Biochemistry and Biotechnology - Tập 165 - Trang 938-948 - 2011
Crude oil-contaminated soil samples were gathered across Khuzestan oilfields (National Iranian South Oil Company, NISOC) consequently experienced a screening procedure for isolating C–S targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. Among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a C–S target...... hiện toàn bộ
Sulfate inhibition of photosystem II oxygen evolving complex
Applied Biochemistry and Biotechnology - - 1987
Anti-Bactericidal Properties of Stingray Dasyatis pastinaca Groups V, IIA, and IB Phospholipases A2: A Comparative Study
Applied Biochemistry and Biotechnology - Tập 174 - Trang 1520-1534 - 2014
Group IIA secreted phospholipase A2 (group IIA sPLA2) is known to display potent Gram-positive bactericidal activity in vitro and in vivo. We have analyzed the bactericidal activity of the full set of native stingray and dromedary groups V, IIA, and IB sPLA2s on several Gram-positive and Gram-negative strains. The rank order potency among both marine and mammal sPLA2s against Gram-positive bacteri...... hiện toàn bộ
Purification and Biochemical Characterization of Polyphenol Oxidases from Embryogenic and Nonembryogenic Cotton (Gossypium hirsutum L.) Cells
Applied Biochemistry and Biotechnology - Tập 158 - Trang 285-301 - 2008
Polyphenol oxidases (PPOs) were isolated from cell suspensions of two cultivars of cotton (Gossypium hirsutum L.), and their biochemical characteristics were studied. PPO from Coker 312, an embryogenic cultivar, showed a highest affinity to catechol 20 mM, and PPO from R405-2000, a nonembryogenic cultivar, showed a highest affinity to 4-methylcatechol 20 mM. The optimal pH for PPO activity was 7.0...... hiện toàn bộ
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