Applied Biochemistry and Biotechnology
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Purification and Biochemical Characterization of Polyphenol Oxidases from Embryogenic and Nonembryogenic Cotton (Gossypium hirsutum L.) Cells
Applied Biochemistry and Biotechnology - Tập 158 - Trang 285-301 - 2008
Polyphenol oxidases (PPOs) were isolated from cell suspensions of two cultivars of cotton (Gossypium hirsutum L.), and their biochemical characteristics were studied. PPO from Coker 312, an embryogenic cultivar, showed a highest affinity to catechol 20 mM, and PPO from R405-2000, a nonembryogenic cultivar, showed a highest affinity to 4-methylcatechol 20 mM. The optimal pH for PPO activity was 7.0......
Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant
Applied Biochemistry and Biotechnology - Tập 182 - Trang 1630-1641 - 2017
Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of e......
Manipulation of microalgal lipid production using genetic engineering
Applied Biochemistry and Biotechnology - Tập 57 - Trang 223-231 - 1996
Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase) gene from the diatomCyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was produced by t......
Comparative Bioactive Studies Between Wild Plant and Callus Culture of Tephrosia tinctoria Pers.
Applied Biochemistry and Biotechnology - Tập 171 - Trang 2105-2120 - 2013
Tephrosia tinctoria, a perennial under shrub of Fabaceae family, is endemic to Western Ghats. In this study, friable whitish yellow callus was developed after 45 days using Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2.0 mg/l) + 6-benzylaminopurine (0.5 mg/l) in various explants of T. tinctoria. The ethyl acetate extracts of leaf (LE), stem (SE), a......
Evaluation of Anticancer Activities of Novel Facile Synthesized Calix[n]arene Sulfonamide Analogs
Applied Biochemistry and Biotechnology - Tập 190 - Trang 1484-1497 - 2019
Here, new calixarene sulfonamide analogs were synthesized from the reaction of chlorosulfonated calix[n]arene (n: 4, 6, and 8) with N,N′-dimethylethylenediamine or ethylenediamine for the first time and an excellent calixarene sulfonamide analog showing potent and selective cytotoxic activity on some cancer cell lines were discovered. Cytotoxicity of the prepared calix[n]arene sulfonamide analogs ......
Comparative study of methods for extraction and purification of environmental DNA from soil and sludge samples
Applied Biochemistry and Biotechnology - Tập 134 - Trang 97-112 - 2006
An important prerequisite for successful construction of a metagenome library is an efficient procedure for extracting DNA from environmental samples. We compared three indirect and four direct extraction methods, including a commercial kit, in terms of DNA yield, purity, and time requirement. A special focus was on methods that are appropriate for the extraction of environmental DNA (eDNA) from v......
Toxic effects of selected industrial solvents in batch and continuous anaerobic reactors
Applied Biochemistry and Biotechnology - Tập 28 - Trang 297-305 - 1991
Acetone and ethyl acetate were readily degraded in batch reactors containing anaerobic solids from an industrial reactor at concentrations to 1000 mg/L; isoamyl acetate was degraded at concentrations below 200 mg/L. Xylene was noninhibitory in batch cultures at concentrations of 50 mg/L or less. Batch reactors receiving 45 mg/L methylene chloride (MC) experienced a 60% reduction in gas production,......
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant
Applied Biochemistry and Biotechnology - - 1992
Using cellulase/hemicellulase complex ofSclerotium rolfsii UV-8 mutant andSaccharomyces cerevisiae for fermentation, the coupled saccharification/ fermentation (CSF) of 15% AT-rice straw was carried out at 40°C, pH 4.5 for the first 24 h and further incubation was performed at 30°C for 72 h. Increasing the amount of cellulase activity from 3–12 IU FPA/g of substrate resulted in increased yields of......
Prediction of Skin Disease with Three Different Feature Selection Techniques Using Stacking Ensemble Method
Applied Biochemistry and Biotechnology - Tập 191 - Trang 637-656 - 2019
Skin disease is the most common problem between people. Due to pollution and deployment of ozone layer, harmful UV rays of sun burn the skin and develop various types of skin diseases. Nowadays, machine learning and deep learning algorithms are generally used for diagnosis for various kinds of diseases. In this study, we have applied three feature extraction techniques univariate feature selection......
Enantioselective Resolution of γ-Lactam by a Novel Thermostable Type II (+)-γ-Lactamase from the Hyperthermophilic Archaeon Aeropyrum pernix
Applied Biochemistry and Biotechnology - Tập 176 - Trang 170-184 - 2015
A thermostable formamidase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was revealed a novel type II (+)-γ-lactamase. This type II (+)-γ-lactamase is only composed of 377 amino acid residues, in contrast to another thermostable (+)-γ-lactamase from Sulfolobus solfataricus with 504 amino acid residues (type I). It is interesting that there are low identities between these two (+)-γ-......
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