Applied Biochemistry and Biotechnology

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Purification and Biochemical Characterization of Polyphenol Oxidases from Embryogenic and Nonembryogenic Cotton (Gossypium hirsutum L.) Cells
Applied Biochemistry and Biotechnology - Tập 158 - Trang 285-301 - 2008
Tanoh Hilaire Kouakou, Yatty Justin Kouadio, Patrice Kouamé, Pierre Waffo-Téguo, Alain Décendit, Jean-Michel Mérillon
Polyphenol oxidases (PPOs) were isolated from cell suspensions of two cultivars of cotton (Gossypium hirsutum L.), and their biochemical characteristics were studied. PPO from Coker 312, an embryogenic cultivar, showed a highest affinity to catechol 20 mM, and PPO from R405-2000, a nonembryogenic cultivar, showed a highest affinity to 4-methylcatechol 20 mM. The optimal pH for PPO activity was 7.0... hiện toàn bộ
Effector-assisted refolding of recombinant tissue-plasminogen activator produced in escherichia coli
Applied Biochemistry and Biotechnology - Tập 33 - Trang 117-138 - 1992
H. Grcinfeld, A. Patel, A. Shatzman, A. H. Nishikawa
Recombinant tissue-plasminogen activator (r-tPA), expressed inEscherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the functional folding and activity of the native protein. The use of partially purified ... hiện toàn bộ
The Effects of Wheat Bran Composition on the Production of Biomass-Hydrolyzing Enzymes by Penicillium decumbens
Applied Biochemistry and Biotechnology - Tập 146 - Trang 119-128 - 2007
Xianyun Sun, Ziyong Liu, Yinbo Qu, Xuezhi Li
The effects of the starch, protein, and soluble oligosaccharides contents in wheat bran on the extracellular biomass-hydrolyzing enzymes activities released by Penicillium decumbens mycelia grown in batch fermentations have been examined. The results showed increased starch content correlated directly with an increase in released amylase activity but inversely with the levels of secreted cellulase... hiện toàn bộ
Application of a depolymerization model for predicting thermochemical hydrolysis of hemicellulose
Applied Biochemistry and Biotechnology - Tập 105 - Trang 53-67 - 2003
Todd Lloyd, Charles E. Wyman
Literature data were collected and analyzed to guide selection of conditions for pretreatment by dilute acid and water-only hemicellulose hydrolysis, and the severity parameter was used to relate performance of different studies on a consistent basis and define attractive operating conditions. Experiments were then run to confirm performance with corn stover. Although substantially better hemicell... hiện toàn bộ
Lignosulfonate biodegradation by Chrysonilia Sitophila
Applied Biochemistry and Biotechnology - Tập 30 Số 2 - Trang 185-192 - 1991
Jaime Rodrı́guez, Nelsón Durán
Tyrosine Residues 232 and 401 Play a Critical Role in the Binding of the Cofactor FAD of Acyl-coA Oxidase
Applied Biochemistry and Biotechnology - - 2018
Shuai Deng, Ping Li, Yiping Wang, Jia Zeng
Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant
Applied Biochemistry and Biotechnology - Tập 182 - Trang 1630-1641 - 2017
Xue-chao Hu, Wei-ming Liu, Miao-miao Luo, Lu-jing Ren, Xiao-jun Ji, He Huang
Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of extracellular MK-7. MK-7 y... hiện toàn bộ
Lipase-catalyzed Reactions at Interfaces of Two-phase Systems and Microemulsions
Applied Biochemistry and Biotechnology - Tập 158 - Trang 706-721 - 2008
P. Reis, R. Miller, M. Leser, H. Watzke
This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil–water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from ... hiện toàn bộ
Isolation and Phytochemical Screening of Endophytic Fungi Isolated from Medicinal Plant Mappia foetida and Evaluation of Its In Vitro Cytotoxicity in Cancer
Applied Biochemistry and Biotechnology - Tập 194 - Trang 4570-4586 - 2022
Pooja Ravi, Prathap Somu, Diptikanta Acharya, Levin Anbu Gomez, Jesse Joel Thathapudi, Yerappa Lakshmikanth Ramachandra, Sunitha Bommanahalli Rudraiah, Mona Isaq, Chetan Shekhar Karua, Mohammed Arifullah, Chandrappa Chinna Poojari, Yong Rok Lee
Isolated endophyte fungi from Mappia foetida have been explored as a potential source for the mass production of anticancer drug lead compounds in the current study. Since medical plants are not feasible economically for mass production of bioactive pharmaceutical important molecules using plant tissue culture due to factors like media design and fungal contamination, endophyte fungal mass culture... hiện toàn bộ
Exploring the Role of Glycolytic Enzymes PFKFB3 and GAPDH in the Modulation of Aβ and Neurodegeneration and Their Potential of Therapeutic Targets in Alzheimer’s Disease
Applied Biochemistry and Biotechnology - Tập 195 - Trang 4673-4688 - 2023
Imran Ahmad, Ranjana Singh, Saurabh Pal, Soni Prajapati, Nidhi Sachan, Yusra Laiq, Hadiya Husain
Alzheimer’s disease (AD) is presently the 6th major cause of mortality across the globe. However, it is expected to rise rapidly, following cancer and heart disease, as a leading cause of death among the elderly peoples. AD is largely characterized by metabolic changes linked to glucose metabolism and age-induced mitochondrial failure. Recent research suggests that the glycolytic pathway is requir... hiện toàn bộ
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