American Journal of Physiology - Cell Physiology

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Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel
American Journal of Physiology - Cell Physiology - Tập 268 Số 5 - Trang C1157-C1163 - 1995
Fiona J. McDonald, Margaret P. Price, Peter M. Snyder, Michael J. Welsh
Amiloride-sensitive Na+ channels are an important component of the Na+ reabsorption pathway in a number of epithelia. Here we report the cloning and characterization of cDNAs encoding two subunits of the human kidney epithelial Na+ channel (beta- and gamma-hENaC). Their predicted amino acid sequences were highly homologous (83-85% identical) to the corresponding subunits reported from rat...... hiện toàn bộ
Ca(2+)-dependent Cl- channels in undifferentiated human colonic cells (HT-29). II. Regulation and rundown
American Journal of Physiology - Cell Physiology - Tập 264 Số 4 - Trang C977-C985 - 1993
Andrew P. Morris, R. A. Frizzell
The regulation of 15-pS Cl- channels by Ca(2+)-mobilizing agonists was investigated by simultaneous cell-attached patch and intracellular Ca2+ concentration ([Ca2+]i) measurements. Cells were loaded with a synthetic peptide made from the calmodulin binding domain of Ca2+/calmodulin-dependent protein kinase II. This caused inhibition of Cl- channel activity without any corresponding effect...... hiện toàn bộ
Turnover of adenosine in plasma of human and dog blood
American Journal of Physiology - Cell Physiology - Tập 256 Số 4 - Trang C799-C806 - 1989
Georg Möser, Jürgen Schrader, Andreas Deußen
To determine half-life and turnover of plasma adenosine, heparinized blood from healthy volunteers was incubated with radiolabeled adenosine in the physiological concentration range of 0.1-1 microM. Plasma levels of adenosine in vitro were 82 +/- 14 nM and were similar to those determined immediately after blood collection with a "stopping solution." Dipyridamole (83 microM) and erythro-9...... hiện toàn bộ
Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and increased mitochondrial Bcl-2
American Journal of Physiology - Cell Physiology - Tập 294 Số 6 - Trang C1509-C1520 - 2008
Voraratt Champattanachai, Richard B. Marchase, John C. Chatham
We have previously reported that glucosamine protected neonatal rat ventricular myocytes against ischemia-reperfusion (I/R) injury, and this was associated with an increase in protein O-linked- N-acetylglucosamine ( O-GlcNAc) levels. However, the protective effect of glucosamine could be mediated via pathways other that O-GlcNAc formation; thus the initial goal of the present study was to...... hiện toàn bộ
15-Lipoxygenase products of arachidonate play a role in proliferation of transformed erythroid cells
American Journal of Physiology - Cell Physiology - Tập 259 Số 6 - Trang C849-C853 - 1990
D. Postoak, Leslie Nystuen, Lucy Jane King, M. Ueno, Barbara S. Beckman
The role of specific products of the lipoxygenase pathway of arachidonic acid metabolism has been investigated in the Friend erythroleukemia cell line, a model system for erythroid cell differentiation. When triggered with agents such as hexamethylene-bis-acetamide, these cells mature as normal erythroid cells. 15-Hydroxyeicosatetraenoic acid (15-HETE) was identified by reverse-phase high...... hiện toàn bộ
Expression of glucocorticoid receptor α- and β-isoforms in human cells and tissues
American Journal of Physiology - Cell Physiology - Tập 283 Số 4 - Trang C1324-C1331 - 2002
Laura Pujols, Joaquim Mullol, Jordi Roca‐Ferrer, Alfons Torrego, Antoni Xaubet, John A. Cidlowski, César Picado
Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR-α and GR-β. We investigated the expression of both GR isoforms in healthy human cells and tissues. GR-α mRNA abundance (×106 cDNA copies/μg total RNA) was as follows: brain (3.83 ± 0.80) > skeletal muscle > macrophages > lung > kidney > l...... hiện toàn bộ
IFN-γ + LPS induction of iNOS is modulated by ERK, JNK/SAPK, and p38 mapk in a mouse macrophage cell line
American Journal of Physiology - Cell Physiology - Tập 280 Số 3 - Trang C441-C450 - 2001
Edward D. Chan, David W. H. Riches
Nitric oxide (NO·) produced by inducible nitric oxide synthase (iNOS) mediates a number of important physiological and pathophysiological processes. The objective of this investigation was to examine the role of mitogen-activated protein kinases (MAPKs) in the regulation of iNOS and NO· by interferon-γ (IFN-γ) + lipopolysaccharide (LPS) in macrophages using specific inhibitors and dominan...... hiện toàn bộ
Gsα deficiency in skeletal muscle leads to reduced muscle mass, fiber-type switching, and glucose intolerance without insulin resistance or deficiency
American Journal of Physiology - Cell Physiology - Tập 296 Số 4 - Trang C930-C940 - 2009
Min Chen, Han‐Zhong Feng, Divakar Gupta, James Kelleher, Kathryn E. Dickerson, Jie Wang, Desmond Hunt, William Jou, Oksana Gavrilova, Jian‐Ping Jin, Lee S. Weinstein
The ubiquitously expressed G protein α-subunit Gsα is required for receptor-stimulated intracellular cAMP responses and is an important regulator of energy and glucose metabolism. We have generated skeletal muscle-specific Gsα-knockout (KO) mice (MGsKO) by mating Gsα-floxed mice with muscle creatine kinase-cre transgenic mice....... hiện toàn bộ
Beta-adrenergic stimulation and cAMP mobilize Ca2+ from an IP3-insensitive pool in rat submandibular granular ducts
American Journal of Physiology - Cell Physiology - Tập 265 Số 5 - Trang C1356-C1362 - 1993
J.P. Dehaye, Ingrid H. Valdez, Richard Turner
The beta-adrenergic agonist isoproterenol induced an increase in intracellular calcium concentration ([Ca2+]i) in rat submandibular granular ducts that was blocked by beta-adrenergic but not by alpha-adrenergic or muscarinic antagonists. This effect was only partially inhibited by the selective beta 1- and beta 2-adrenergic antagonists atenolol and ICI-118,551, but was completely blocked ...... hiện toàn bộ
Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies
American Journal of Physiology - Cell Physiology - Tập 269 Số 6 - Trang C1496-C1505 - 1995
Christian Lytle, Jun Xu, Daniel Biemesderfer, Bliss Forbush
The Na-K-Cl cotransporter (NKCC) is present in most animal cells where it functions in cell volume homeostasis and epithelial salt transport. We developed six monoclonal antibodies (designated T4, T8, T9, T10, T12, and T14) against a fusion protein fragment encompassing the carboxy-terminal 310 amino acids of the human colonic NKCC. These T antibodies selectively recognized putative NKCC ...... hiện toàn bộ
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