American Journal of Physiology - Cell Physiology
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Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel
American Journal of Physiology - Cell Physiology - Tập 268 Số 5 - Trang C1157-C1163 - 1995
Amiloride-sensitive Na+ channels are an important component of the Na+
reabsorption pathway in a number of epithelia. Here we report the cloning and
characterization of cDNAs encoding two subunits of the human kidney epithelial
Na+ channel (beta- and gamma-hENaC). Their predicted amino acid sequences were
highly homologous (83-85% identical) to the corresponding subunits reported from
rat colon (b...
Ca(2+)-dependent Cl- channels in undifferentiated human colonic cells (HT-29). II. Regulation and rundown
American Journal of Physiology - Cell Physiology - Tập 264 Số 4 - Trang C977-C985 - 1993
The regulation of 15-pS Cl- channels by Ca(2+)-mobilizing agonists was
investigated by simultaneous cell-attached patch and intracellular Ca2+
concentration ([Ca2+]i) measurements. Cells were loaded with a synthetic peptide
made from the calmodulin binding domain of Ca2+/calmodulin-dependent protein
kinase II. This caused inhibition of Cl- channel activity without any
corresponding effect on eithe...
Turnover of adenosine in plasma of human and dog blood
American Journal of Physiology - Cell Physiology - Tập 256 Số 4 - Trang C799-C806 - 1989
To determine half-life and turnover of plasma adenosine, heparinized blood from
healthy volunteers was incubated with radiolabeled adenosine in the
physiological concentration range of 0.1-1 microM. Plasma levels of adenosine in
vitro were 82 +/- 14 nM and were similar to those determined immediately after
blood collection with a "stopping solution." Dipyridamole (83 microM) and
erythro-9(2-hydrox...
Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and increased mitochondrial Bcl-2
American Journal of Physiology - Cell Physiology - Tập 294 Số 6 - Trang C1509-C1520 - 2008
We have previously reported that glucosamine protected neonatal rat ventricular
myocytes against ischemia-reperfusion (I/R) injury, and this was associated with
an increase in protein O-linked- N-acetylglucosamine ( O-GlcNAc) levels.
However, the protective effect of glucosamine could be mediated via pathways
other that O-GlcNAc formation; thus the initial goal of the present study was to
determin...
15-Lipoxygenase products of arachidonate play a role in proliferation of transformed erythroid cells
American Journal of Physiology - Cell Physiology - Tập 259 Số 6 - Trang C849-C853 - 1990
The role of specific products of the lipoxygenase pathway of arachidonic acid
metabolism has been investigated in the Friend erythroleukemia cell line, a
model system for erythroid cell differentiation. When triggered with agents such
as hexamethylene-bis-acetamide, these cells mature as normal erythroid cells.
15-Hydroxyeicosatetraenoic acid (15-HETE) was identified by reverse-phase
high-performa...
Expression of glucocorticoid receptor α- and β-isoforms in human cells and tissues
American Journal of Physiology - Cell Physiology - Tập 283 Số 4 - Trang C1324-C1331 - 2002
Alternative splicing of the human glucocorticoid receptor (GR) primary
transcript generates two protein isoforms: GR-α and GR-β. We investigated the
expression of both GR isoforms in healthy human cells and tissues. GR-α mRNA
abundance (×106 cDNA copies/μg total RNA) was as follows: brain (3.83 ± 0.80) >
skeletal muscle > macrophages > lung > kidney > liver > heart > eosinophils >
peripheral blood...
IFN-γ + LPS induction of iNOS is modulated by ERK, JNK/SAPK, and p38 mapk in a mouse macrophage cell line
American Journal of Physiology - Cell Physiology - Tập 280 Số 3 - Trang C441-C450 - 2001
Nitric oxide (NO·) produced by inducible nitric oxide synthase (iNOS) mediates a
number of important physiological and pathophysiological processes. The
objective of this investigation was to examine the role of mitogen-activated
protein kinases (MAPKs) in the regulation of iNOS and NO· by interferon-γ
(IFN-γ) + lipopolysaccharide (LPS) in macrophages using specific inhibitors and
dominant inhibit...
Gsα deficiency in skeletal muscle leads to reduced muscle mass, fiber-type switching, and glucose intolerance without insulin resistance or deficiency
American Journal of Physiology - Cell Physiology - Tập 296 Số 4 - Trang C930-C940 - 2009
The ubiquitously expressed G protein α-subunit Gsα is required for
receptor-stimulated intracellular cAMP responses and is an important regulator
of energy and glucose metabolism. We have generated skeletal muscle-specific
Gsα-knockout (KO) mice (MGsKO) by mating Gsα-floxed mice with muscle creatine
kinase-cre transgenic mice. MGsKO mice had normal body weight and composition,
and their serum gluc...
Beta-adrenergic stimulation and cAMP mobilize Ca2+ from an IP3-insensitive pool in rat submandibular granular ducts
American Journal of Physiology - Cell Physiology - Tập 265 Số 5 - Trang C1356-C1362 - 1993
The beta-adrenergic agonist isoproterenol induced an increase in intracellular
calcium concentration ([Ca2+]i) in rat submandibular granular ducts that was
blocked by beta-adrenergic but not by alpha-adrenergic or muscarinic
antagonists. This effect was only partially inhibited by the selective beta 1-
and beta 2-adrenergic antagonists atenolol and ICI-118,551, but was completely
blocked by the co...
Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies
American Journal of Physiology - Cell Physiology - Tập 269 Số 6 - Trang C1496-C1505 - 1995
The Na-K-Cl cotransporter (NKCC) is present in most animal cells where it
functions in cell volume homeostasis and epithelial salt transport. We developed
six monoclonal antibodies (designated T4, T8, T9, T10, T12, and T14) against a
fusion protein fragment encompassing the carboxy-terminal 310 amino acids of the
human colonic NKCC. These T antibodies selectively recognized putative NKCC
proteins ...
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