American Journal of Physiology - Cell Physiology
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Ca(2+)-dependent Cl- channels in undifferentiated human colonic cells (HT-29). II. Regulation and rundown The regulation of 15-pS Cl- channels by Ca(2+)-mobilizing agonists was investigated by simultaneous cell-attached patch and intracellular Ca2+ concentration ([Ca2+]i) measurements. Cells were loaded with a synthetic peptide made from the calmodulin binding domain of Ca2+/calmodulin-dependent protein kinase II. This caused inhibition of Cl- channel activity without any corresponding effect... ...
American Journal of Physiology - Cell Physiology - Tập 264 Số 4 - Trang C977-C985 - 1993
Turnover of adenosine in plasma of human and dog blood To determine half-life and turnover of plasma adenosine, heparinized blood from healthy volunteers was incubated with radiolabeled adenosine in the physiological concentration range of 0.1-1 microM. Plasma levels of adenosine in vitro were 82 +/- 14 nM and were similar to those determined immediately after blood collection with a "stopping solution." Dipyridamole (83 microM) and erythro-9... ...
American Journal of Physiology - Cell Physiology - Tập 256 Số 4 - Trang C799-C806 - 1989
Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and increased mitochondrial Bcl-2 We have previously reported that glucosamine protected neonatal rat ventricular myocytes against ischemia-reperfusion (I/R) injury, and this was associated with an increase in protein O-linked- N-acetylglucosamine ( O-GlcNAc) levels. However, the protective effect of glucosamine could be mediated via pathways other that O-GlcNAc formation; thus the initial goal of the present study was to... ...
American Journal of Physiology - Cell Physiology - Tập 294 Số 6 - Trang C1509-C1520 - 2008
15-Lipoxygenase products of arachidonate play a role in proliferation of transformed erythroid cells The role of specific products of the lipoxygenase pathway of arachidonic acid metabolism has been investigated in the Friend erythroleukemia cell line, a model system for erythroid cell differentiation. When triggered with agents such as hexamethylene-bis-acetamide, these cells mature as normal erythroid cells. 15-Hydroxyeicosatetraenoic acid (15-HETE) was identified by reverse-phase high... ...
American Journal of Physiology - Cell Physiology - Tập 259 Số 6 - Trang C849-C853 - 1990
Expression of glucocorticoid receptor α- and β-isoforms in human cells and tissues Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR-α and GR-β. We investigated the expression of both GR isoforms in healthy human cells and tissues. GR-α mRNA abundance (×106 cDNA copies/μg total RNA) was as follows: brain (3.83 ± 0.80) > skeletal muscle > macrophages > lung > kidney > l... ...
American Journal of Physiology - Cell Physiology - Tập 283 Số 4 - Trang C1324-C1331 - 2002
IFN-γ + LPS induction of iNOS is modulated by ERK, JNK/SAPK, and p38 mapk in a mouse macrophage cell line Nitric oxide (NO·) produced by inducible nitric oxide synthase (iNOS) mediates a number of important physiological and pathophysiological processes. The objective of this investigation was to examine the role of mitogen-activated protein kinases (MAPKs) in the regulation of iNOS and NO· by interferon-γ (IFN-γ) + lipopolysaccharide (LPS) in macrophages using specific inhibitors and dominan... ...
American Journal of Physiology - Cell Physiology - Tập 280 Số 3 - Trang C441-C450 - 2001
Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies The Na-K-Cl cotransporter (NKCC) is present in most animal cells where it functions in cell volume homeostasis and epithelial salt transport. We developed six monoclonal antibodies (designated T4, T8, T9, T10, T12, and T14) against a fusion protein fragment encompassing the carboxy-terminal 310 amino acids of the human colonic NKCC. These T antibodies selectively recognized putative NKCC ... ...
American Journal of Physiology - Cell Physiology - Tập 269 Số 6 - Trang C1496-C1505 - 1995
Plasticity and stressor specificity of osmotic and heat shock responses of Gillichthys mirabilis gill cells Short-term effects of osmotic and heat shock on proteins of Gillichthys mirabilis gill cells were analyzed. The protein synthesis rate (PSR) of gill cells was influenced by hyperosmotic shock (335-->635 mosmol/kgH2O) and heat shock (25-->37 degrees C), but not by hyposmotic shock (335-->190 mosmol/kgH2O). Between 4 and 6 h after hyperosmotic shock, gill cell protein synthesis was... ...
American Journal of Physiology - Cell Physiology - Tập 271 Số 4 - Trang C1181-C1193 - 1996
Effect of physiological ADP concentrations on contraction of single skinned fibers from rabbit fast and slow muscles To directly assess the possible role of ADP in muscle fatigue, we have studied the effect of physiological MgADP levels on maximum Ca(2+)-activated isometric force and unloaded shortening velocity (Vus) of single skinned fiber segments from rabbit fast-twitch (psoas) and slow-twitch (soleus) muscles. MgADP concentration was changed in a controlled and well-buffered manner by varying creat... ...
American Journal of Physiology - Cell Physiology - Tập 268 Số 2 - Trang C480-C489 - 1995
Intracellular Ca regulation during secretagogue stimulation of the parietal cell Intracellular [Ca] ([Ca]i) was measured following secretagogue stimulation of rabbit gastric glands loaded with the Ca-sensitive dye fura-2. Glands were mounted on cover slips and placed either in a perfused cuvette (for spectrofluorimetric measurements on whole glands) or in a chamber on the stage of a microscope (for microspectrofluorimetric measurements on single parietal cells within ... ...
American Journal of Physiology - Cell Physiology - Tập 254 Số 1 - Trang C130-C140 - 1988
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