Expression of glucocorticoid receptor α- and β-isoforms in human cells and tissues

American Journal of Physiology - Cell Physiology - Tập 283 Số 4 - Trang C1324-C1331 - 2002
Laura Pujols1, Joaquim Mullol1,2, Jordi Roca‐Ferrer1, Alfons Torrego1,3, Antoni Xaubet1,3, John A. Cidlowski4, César Picado1,3
1Institut d'Investigacions Biomèdiques August Pi i Sunyer,
2Servei d'Otorinolaringologia, and
3Servei de Pneumologia i Al.lèrgia Respiratòria-Institut Clı́nic de Pneumologia i Cirurgia Toràcica, Hospital Clı́nic, Departament de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain; and
4Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709

Tóm tắt

Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR-α and GR-β. We investigated the expression of both GR isoforms in healthy human cells and tissues. GR-α mRNA abundance (×106 cDNA copies/μg total RNA) was as follows: brain (3.83 ± 0.80) > skeletal muscle > macrophages > lung > kidney > liver > heart > eosinophils > peripheral blood mononuclear cells (PBMCs) > nasal mucosa > neutrophils > colon (0.33 ± 0.04). GR-β mRNA was much less expressed than GR-α mRNA. Its abundance (×103 cDNA copies/μg total RNA) was as follows: eosinophils (1.55 ± 0.58) > PBMCs > liver ≥ skeletal muscle > kidney > macrophages > lung > neutrophils > brain ≥ nasal mucosa > heart (0.15 ± 0.08). GR-β mRNA was not found in colon. While GR-α protein was detected in all cells and tissues, GR-β was not detected in any specimen. Our results suggest that, in physiological conditions, the default splicing pathway is the one leading to GR-α. The alternative splicing event leading to GR-β is minimally activated.

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American Journal of Physiology - Cell Physiology

Tập 283 Số 4

C1324-C1331

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