N. S. Shcherbakova, A. N. Chikaev, L. I. Karpenko, A. A. Il’ichev
The effect of biotinylation of monoclonal antibodies (MAbs) on the yield and repertoire of peptides selected by biopanning is discussed in the present study. A comparative analysis of peptides selected from a phage library using the biotinylated and nonbiotinylated MAb 2F5 was performed. The yield of the peptides homologous to the native epitope was observed to be 1.7 times higher for the biotinyl...... hiện toàn bộ