Acta Veterinaria Scandinavica

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The Fox (Vulpes vulpes) as a Reservoir for Canine Angiostrongylosis in Denmark
Acta Veterinaria Scandinavica - Tập 33 - Trang 357-362 - 1992
G. Bolt, J. Monrad, P. Henriksen, H. H. Dietz, J. Koch, E. Bindseil, A. L. Jensen
Until recently, Angiostrongylus vasorum was not considered to be an endogenous Danish parasite, since demonstration of this worm had been confined to necropsy findings in 2 dogs, both of which had visited France. During the last 2 years, however, clinical cases have been diagnosed among a considerable number of Danish dogs, none of which had ever been outside Denmark. All these cases have occurred north of Copenhagen, where an endemic focus seems to exist. In this field survey A. vasorum was found for the first time in wild Danish red foxes. Furthermore, experimental infections showed that the parasite can be transferred between foxes and dogs. Consequently, the wild fox population must be considered a potential reservoir for transfer of A. vasorum to domestic dogs.
Challenges and standardization of microRNA profiling in serum and cerebrospinal fluid in dogs suffering from non-infectious inflammatory CNS disease
Acta Veterinaria Scandinavica - Tập 61 Số 1 - 2019
Susanna Cirera, Emilie Andersen-Ranberg, Sille Langkilde, Maria Aaquist, Hanne Gredal
Abstract

Non-infectious inflammatory (NII) central nervous system (CNS) conditions are primarily diagnosed by the demonstration of inflammatory changes in the cerebrospinal fluid (CSF). However, less-invasive methods and peripheral biomarkers are desired. Changes in circulating microRNA (miRNA), which are short non-coding regulatory RNAs, may serve as biomarkers of disease. The aim of this pilot study was to investigate selected miRNAs in serum and CSF, hypothesizing that the levels of specific miRNAs in serum correlate with their presence in CSF, and that changes in serum miRNAs levels may reflect CNS disease. We profiled serum and CSF samples using quantitative real-time PCR (qPCR) searching for selected and previously profiled miRNAs in serum (let-7a, let-7c, miR-15b, miR-16, miR-21, miR-23a, miR-24, miR-26a, miR-146a, miR-155, miR-181c and miR-221-3p) and in CSF (let-7c, miR-16, miR-21, miR-24, miR-146a, miR-155, miR-181c and miR-221-3p) from 13 dogs with NII CNS disease and six control dogs. We demonstrated the presence of several miRNAs in CSF (let-7c and miR-21 dominating) and serum (miR-23a and miR-21 dominating). However, we generally failed to reproduce consistent results in CSF samples due to several reasons: unacceptable PCR efficiency, a wide variation between cDNA replicates and/or no-amplification in qPCR suggesting very low levels of the investigated miRNAs in canine CSF. Serum samples performed better, and 10 miRNAs qPCR assays were qualified for analysis. We were nevertheless unable to detect a difference in the expression of miRNA levels between cases and controls. Moreover, we could not confirm the results of recent miRNA investigations of canine CNS diseases. We believe that these disagreements highlight the significant effect of methodological/analytical variation, rather than the incapacity of circulating miRNAs as biomarkers of CNS disease. A secondary aim was therefore to communicate methodological challenges in our study and to suggest recommendations for circulating miRNA profiling, including pre-, post- and analytical methods based on our experience, in order to reach reproducible and comparable results in veterinary miRNA research.

Methods for Determining the Presence of Tranquilizers and Other New Drugs in Urine of Dogs
Acta Veterinaria Scandinavica - Tập 4 - Trang 209-220 - 1963
Knut Sjöberg, Gunnar Tufvesson
An attempt has been made to work out general methods of determining the presence of recent drugs, among others, tranquilizers. Colour reaction with cone. H2SO4, paper chromatography, and absorption in UV and certain special reactions have been tried. Ten of these substances have been given to dogs. After that attempts have been made to determine the presence of them in the urine from the dogs. Seven of these preparations were identified (for sure.
Clinical Chemical Comparative Examination of Ruminal Samples Collected by Means of a Naso-Ruminal Sampler
Acta Veterinaria Scandinavica - Tập 29 - Trang 129-133 - 1988
J. S. D. Poulsen, K. Özkan, J. O. Andersen
Samples of ruminal fluid collected with the COMET naso-ruminal sampler and by two other methods through fistula were subjected to comparative clinical chemical examination. It was found that the mean values of pH and buffer capacity were nearly identical for all three methods. The greatest variation between the methods were found for reduction time of methylene blue owing to different dry matter contents in the samples. The VFA contents were nearly identical in all three samples. In conclusion, it was found that it is possible to obtain ruminal samples that are representative of the biochemical status in the rumen by means of the COMET naso-ruminal sampler in non-fistulated cows.
Nghiên cứu về Protein trong Plasma Tinh Dịch của Lợn Đực bằng Phương Pháp Điện Di Miễn Dịch và Khuếch Tán Miễn Dịch Dịch bởi AI
Acta Veterinaria Scandinavica - Tập 13 - Trang 449-461 - 1972
Kjell Martinsson, Kerstin Thorén
Các protein trong plasma tinh dịch của lợn đực đã được nghiên cứu bằng các bài kiểm tra điện di miễn dịch và khuếch tán miễn dịch. Bốn protein khác nhau đã được xác định thông qua điện di miễn dịch, tất cả đều dường như đặc trưng cho plasma tinh dịch. Một trong những protein này có độ di động điện di tương ứng với gammaglobulin nhưng không giống với IgG hoặc fibrinogen. Các bài kiểm tra khuếch tán miễn dịch cho thấy có sự hiện diện của protein huyết tương (albumin, transferrin và IgG). Sự kết tủa tự phát của plasma tinh dịch lợn đực trong các gel agar không thể hoàn toàn tránh khỏi với sự thay đổi pH và nồng độ ion trong gel agar hoặc với sự hấp thụ plasma tinh dịch bằng tế bào hồng cầu lợn. Một yếu tố trong plasma tinh dịch, có thể quan trọng cho khả năng tụ tập các tế bào hồng cầu, đã được tách và xác định là một protein có độ di động điện di tương ứng với alphaglobulin. Protein này không thể được phát hiện trong huyết tương.
#protein #plasma tinh dịch #lợn đực #miễn dịch #điện di #khuếch tán miễn dịch
Heavy Metal Concentrations in Dairy Products from Sheep Milk Collected in Two Regions of Southern Italy
Acta Veterinaria Scandinavica - Tập 47 - Trang 1-6 - 2006
Aniello Anastasio, Rosa Caggiano, Maria Macchiato, Catellani Paolo, Maria Ragosta, Salvatore Paino, ML Cortesi
Plasma total antioxidant status in horses after 8-hours of road transportation
Acta Veterinaria Scandinavica - Tập 55 - Trang 1-4 - 2013
Artur Niedźwiedź, Krzysztof Kubiak, Józef Nicpoń
The aim of this study was to investigate the effects of 8-hour road transport on plasma total antioxidant status (PTAS) and general clinical appearance in horses. The study was conducted on a group of 60 horses of different breeds aged from 4 to 10 years. Venous blood was collected and a clinical examination was performed immediately before loading horses onto trailers for an 8 hour transport (I), immediately after unloading them from the trailer (II), and after a subsequent 24 hour stall rest (III). The ferric-reducing ability of plasma (FRAP) was used to determine PTAS. The transportation significantly increased respiratory and heart rates. The average PTAS increased during the three subsequent samplings: I: 170 ± 77 (μmol/l) II: 204 ± 70 (μmol/l) III: 221 ± 74 (μmol/l). Long-distance transport increased the PTAS horses, as well as respiratory and heart rates.
Toxoplasmosis in Sheep
Acta Veterinaria Scandinavica - Tập 17 - Trang 426-431 - 1976
Harald Waldeland
Sera were tested in 2 series both by the conventional technique (dilution in tubes) and by the microtiter system. In 1 series of comparative examinations the tests were performed with the same batch of accessory factor serum and antigen preparation, and in another series the tests were performed at 2 different laboratories. There was a high correlation between the 2 methods, but the titres obtained by the microtiter system were about 1 to 2 twofold dilutions lower than those obtained by the conventional dilution technique. The 95 % confidence limits by the microtiter system were plus and minus 1 twofold dilution.
Vaccination and Eradication Programme against Aujeszky’s Disease in Five Swedish Pig Herds with Special Reference to Herd Owner Attitudes
Acta Veterinaria Scandinavica - Tập 40 - Trang 213-219 - 1999
M. Engel, M. Wierup
A vaccination eradication programme against Aujeszky’s disease (AD), based on the use of gE-negative killed vaccine, was carried out between 1987 and 1992 in 5 Swedish weaner pig producing or farrow-to-finish herds, with 63 to 398 breeding animals. All breeding animals were tested at the start and the end of the programme. Seroprevalence to Aujeszky’s disease virus (ADV) ranged between 47% and 100% in the herds at the first test. During the programme, all breeding animals were vaccinated simultaneously every 4 months and ADV-free replacement animals were vaccinated shortly after arrival and boostered within a month. In one herd only, a limited number of fatteners were vaccinated. The herds were declared free (gE-negative) 12 to 53 months after the start of the programme. When all seropositive breeding animals had been culled, the programme ended after 2 negative tests of the breeding animals. Seroconversion was limited in all herds but one, where initially no isolation unit was available for replacement animals. The attitude of the herd owners towards the programme and the special conditions prevailing in the herds are discussed. It is suggested that vaccination may promote risk behaviour of herd managers.
A Modified IF-test to Demonstrate IgM Antibodies to Babesia Divergens of Cattle
Acta Veterinaria Scandinavica - Tập 28 - Trang 361-371 - 2021
Dan A. Christensson
An IF–test modified to increase sensitivity was used to detect the presence of Babesia divergens specific IgM antibodies. It consisted of three steps: (1) the incubation of Babesia antigen with test serum, followed by; (2) its incubation with protein A; and (3) the addition of rabbit anti-bovine IgM bound to protein A, which was bound with FITC. Compared to the conventional IF–technique, this modified IF-technique detected bovine anti-Babesia-IgM in test serum at a two–fold lower dilution and with a diminished background staining. This modified IF-technique improves the possibilities for studying class Μ antibody in the serum of cattle which have been infected with B. divergens. It is possible to determine whether a calf s antibody response is due to maternally transmitted antibodies or actively acquired antibodies by determining the presence of each of the classes of immunoglobulin against B. divergens present in the calf s serum.
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