miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs

Genes and Development - Tập 16 Số 6 - Trang 720-728 - 2002
Zissimos P. Mourelatos1, Josée Dostie2, Sergey Paushkin2, Anup Sharma2, Julien Royet3, Linda Abel2, Juri Rappsilber4, Matthias Mann4, Gideon Dreyfuss2
1Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148, USA
2Howard Hughes Medical Institute, Department of Biochemistry & Biophysics, and
3Laboratoire de Ge ´ne ´tique et Physiologie du De ´veloppement, CNRS-Universite ´de la Mediterrane ´e, Marseille, Cedex 09, France;
4Protein Interaction Laboratory, University of Southern Denmark, DK-5230 Odense M, Denmark

Tóm tắt

Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6. Reduction in SMN protein results in Spinal muscular atrophy (SMA), a common neurodegenerative disease. The SMN complex has critical functions in the assembly/restructuring of diverse ribonucleoprotein (RNP) complexes. Here we report that Gemin3 and Gemin4 are also in a separate complex that contains eIF2C2, a member of the Argonaute protein family. This novel complex is a large ∼15S RNP that contains numerous microRNAs (miRNAs). We describe 40 miRNAs, a few of which are identical to recently described human miRNAs, a class of small endogenous RNAs. The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem–loop structures.

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Genes and Development

Tập 16 Số 6

720-728

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