iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams

Springer Science and Business Media LLC - Tập 18 Số 1 - 2021
Bin Yu1, Hong-Dan Zhu1, Xiaoliang Shi1, Pan‐Pan Chen2, Xiang-Mei Sun2, Gui-Yu Xia1, Min Fang1, Yong-Xing Zhong1, Xiaoli Tang2, Tao Zhang1, Hai‐Tao Pan2
1Shaoxing Maternity and Child Health Care Hospital, Shaoxing, 312000, China
2Obstetrics and Gynecology Hospital of Shaoxing University, Shaoxing, China

Tóm tắt

Abstract Background Preeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia. Methods To gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS. Results By using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, had the strongest effect on cardiovascular function. The expression of G6PD was upregulated in thoracic aorta tissues, as confirmed by Western blotting. The expression of two other vascular function-related proteins, cysteine- and glycine-rich protein 2 (CSRP2) and tubulin alpha-4 A (TUBA4A), was upregulated, as demonstrated by mass spectrometry (MS). Conclusions Although the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers.

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Tập 18 Số 1

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